Microbial Biodiesel Production - Oil Feedstocks Produced from Microbial Cell Cultivations

Zhang, Jianguo; Hu, Bo

doi:10.5772/25285

Cited by 3 publications

(1 citation statement)

References 74 publications

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“…However, for low-value biodiesel application, low cost substrates, such as food rest materials, waste glycerol and lignocellulosic materials are being tested for their economical sustainability. Interestingly, fungi (yeast and molds) are able to grow and accumulate lipids on such substrates [ 8 – 11 ].…”

Section: Introductionmentioning

confidence: 99%

High-throughput screening of Mucoromycota fungi for production of low- and high-value lipids

Kósa

Zimmermann

Köhler

et al. 2018

Biotechnol Biofuels

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BackgroundMucoromycota fungi are important producers of low- and high-value lipids. Mortierella alpina is used for arachidonic acid production at industrial scale. In addition, oleaginous Mucoromycota fungi are promising candidates for biodiesel production. A critical step in the development of such biotechnological applications is the selection of suitable strains for lipid production. The aim of the present study was to use the Duetz-microtiter plate system combined with Fourier transform infrared (FTIR) spectroscopy for high-throughput screening of the potential of 100 Mucoromycota strains to produce low- and high-value lipids.ResultsWith this reproducible, high-throughput method, we found several promising strains for high-value omega-6 polyunsaturated fatty acid (PUFA) and biodiesel production purposes. Gamma-linolenic acid content was the highest in Mucor fragilis UBOCC-A-109196 (24.5% of total fatty acids), and Cunninghamella echinulata VKM F-470 (24.0%). For the first time, we observed concomitant gamma-linolenic acid and alpha-linolenic acid (up to 13.0%) production in psychrophilic Mucor flavus strains. Arachidonic acid was present the highest amount in M. alpina ATCC 32222 (41.1% of total fatty acids). Low cultivation temperature (15 °C) activated the temperature sensitive ∆17 desaturase enzyme in Mortierella spp., resulting in eicosapentaenoic acid production with up to 11.0% of total fatty acids in M. humilis VKM F-1494. Cunninghamella blakesleeana CCM-705, Umbelopsis vinacea CCM F-539 and UBOCC-A-101347 showed very good growth (23–26 g/L) and lipid production (7.0–8.3 g/L) with high palmitic and oleic acid, and low PUFA content, which makes them attractive candidates for biodiesel production. Absidia glauca CCM 451 had the highest total lipid content (47.2% of biomass) of all tested strains. We also demonstrated the potential of FTIR spectroscopy for high-throughput screening of total lipid content of oleaginous fungi.ConclusionsThe use of Duetz-microtiter plate system combined with FTIR spectroscopy and multivariate analysis, is a feasible approach for high-throughput screening of lipid production in Mucoromycota fungi. Several promising strains have been identified by this method for the production of high-value PUFA and biodiesel.Electronic supplementary materialThe online version of this article (10.1186/s13068-018-1070-7) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

High-throughput screening of Mucoromycota fungi for production of low- and high-value lipids

Kósa

Zimmermann

Köhler

et al. 2018

Biotechnol Biofuels

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Search for Future Fuels—Pathway Points to a ‘Boring’ Process

Santhakumaran

2017

Wood Is Good

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Immobilization ofPseudomonas fluorescenslipase on hydrophobic supports and application in biodiesel synthesis by transesterification of vegetable oils in solvent-free systems

Lima

Oliveira

Rojas

et al. 2015

Journal of Industrial Microbiology and Biotechnology

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This work describes the preparation of biocatalysts for ethanolysis of soybean and babassu oils in solvent-free systems. Polystyrene, Amberlite (XAD-7HP), and octyl-silica were tested as supports for the immobilization of Pseudomonas fluorescens lipase (PFL). The use of octyl-silica resulted in a biocatalyst with high values of hydrolytic activity (650.0 ± 15.5 IU/g), immobilization yield (91.3 ± 0.3 %), and recovered activity (82.1 ± 1.5 %). PFL immobilized on octyl-silica was around 12-fold more stable than soluble PFL, at 45 °C and pH 8.0, in the presence of ethanol at 36 % (v/v). The biocatalyst provided high vegetable oil transesterification yields of around 97.5 % after 24 h of reaction using babassu oil and around 80 % after 48 h of reaction using soybean oil. The PFL-octyl-silica biocatalyst retained around 90 % of its initial activity after five cycles of transesterification of soybean oil. Octyl-silica is a promising support that can be used to immobilize PFL for subsequent application in biodiesel synthesis.

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Microbial Biodiesel Production - Oil Feedstocks Produced from Microbial Cell Cultivations

Cited by 3 publications

References 74 publications

High-throughput screening of Mucoromycota fungi for production of low- and high-value lipids

High-throughput screening of Mucoromycota fungi for production of low- and high-value lipids

Search for Future Fuels—Pathway Points to a ‘Boring’ Process

Immobilization ofPseudomonas fluorescenslipase on hydrophobic supports and application in biodiesel synthesis by transesterification of vegetable oils in solvent-free systems

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