Microbial Biofertilizer Decreases Nicotine Content by Improving Soil Nitrogen Supply

Abstract

Biofertilizers have been widely used in many countries for their benefit to soil biological and physicochemical properties. A new microbial biofertilizer containing Phanerochaete chrysosporium and Bacillus thuringiensis was prepared to decrease nicotine content in tobacco leaves by regulating soil nitrogen supply. Soil NO-N, NH-N, nitrogen supply-related enzyme activities, and nitrogen accumulation in plant leaves throughout the growing period were investigated to explore the mechanism of nicotine reduction. The experimental results indicated that biofertilizer can reduce the nicotine content in tobacco leaves, with a maximum decrement of 16-18 % in mature upper leaves. In the meantime, the total nitrogen in mature lower and middle leaves increased with the application of biofertilizer, while an opposite result was observed in upper leaves. Protein concentration in leaves had similar fluctuation to that of total nitrogen in response to biofertilizer. NO-N content and nitrate reductase activity in biofertilizer-amended soil increased by 92.3 and 42.2 %, respectively, compared to those in the control, whereas the NH-N and urease activity decreased by 37.8 and 29.3 %, respectively. Nitrogen uptake was improved in the early growing stage, but this phenomenon was not observed during the late growth period. Nicotine decrease is attributing to the adjustment of biofertilizer in soil nitrogen supply and its uptake in tobacco, which result in changes of nitrogen content as well as its distribution in tobacco leaves. The application of biofertilizer containing P. chrysosporium and B. thuringiensis can reduce the nicotine content and improve tobacco quality, which may provide some useful information for tobacco cultivation.

Influences of Drought and Microbial Water‐Retention Fertilizer on Leaf Area Index and Photosynthetic Characteristics of Flue‐Cured Tobacco

“…Shang et al . () found that the application of biofertilizer containing Phanerochaete chrysosporium and Bacillus thuringiensis could reduce the nicotine content and improve tobacco quality, which might provide some useful information for tobacco. All of those studies showed that the microbial fertilizer could promote tobacco growth and increase leaf yield.…”

“…Mao et al (2016) found that the conventional fertilization with the Engran microbial agent could promote the growth and dry matter accumulation of flue-cured tobacco during the growth period in the field, enhance the disease resistance of tobacco, and improve yield and output value of flue-cured tobacco and income of tobacco growers. Shang et al (2017) found that the application of biofertilizer containing Phanerochaete chrysosporium and Bacillus thuringiensis could reduce the nicotine content and improve tobacco quality, which might provide some useful information for tobacco. All of those studies showed that the microbial fertilizer could promote tobacco growth and increase leaf yield.…”

Trypsin Binding with Copper Ions Scavenges Superoxide: Molecular Dynamics-Based Mechanism Investigation

“…Its excessive activity has been strongly implicated in acute pancreatitis, inflammation and tumors [ 8 , 9 , 10 ]. Trypsin has recently been proved to be a novel superoxide scavenger in intracorporal and extracorporal systems [ 11 , 12 ]. As an endogenous human enzyme, the superoxide scavenging activities of trypsin make it possible to act as a novel agent to eliminate the damage to cells induced by polychlorinated biphenyls (PCBs).…”

A Seed Mucilage-Degrading Fungus From the Rhizosphere Strengthens the Plant-Soil-Microbe Continuum and Potentially Regulates Root Nutrients of a Cold Desert Shrub

“…Inoculation of tobacco (Nicotiana tabacum) with P. chrysosporium increases nitrate content in soil by promoting microbial nitrification and increasing nitrate reductase activity (Shang et al 2017). Therefore, the increase in nitrate content promotes plant nitrogen uptake and increases root nitrogen content in a nitrogen-limited soil such as that in Mu Us Sandland.…”