Microbial chitin extraction and characterization from green tiger shrimp waste: A comparative study of culture mediums along with bioprocess optimization

Gharibzadeh, Mahsa; Osfouri, Shahriar; Jamekhorshid, Ahmad; Jafari, Seyed Ali

doi:10.1016/j.ijbiomac.2023.125213

Cited by 6 publications

(1 citation statement)

References 49 publications

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“…These results exceed those reported by Ta et al [37] who achieved an 80.9% deproteinization after two days of incubation using Yarrowia lipolytica and achieved a demineralization range of 38.2-49.4% with shrimp head waste. In contrast, Gharibzadeh et al [38] reached a maximum value of 91.22% deproteinization using Bacillus licheniformis at 45 • C and 96.55% demineralization using Lactobacillus plantarum at 37 • C, both during a single-step process. Zare et al [39] reported that with 10% glucose, they achieved 81% demineralization and 62% deproteinization from shrimp shell waste using co-fermentation with Lactiplantibacillus plantarum PTCC 1745 and Bacillus subtilis PTCC 1720.…”

Section: Resultsmentioning

confidence: 88%

Efficient Chitin Extraction from Shrimp Exoskeletons through Single-Step Fermentation by Pseudomonas aeruginosa QF50 and Serratia sp. QCS23

Quiñones-Cerna,

Rodríguez-Soto,

Hurtado-Butrón

et al. 2024

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Chitin, an abundant biopolymer with potential applications in agriculture, medicine, and bioremediation, is conventionally extracted using chemical methods that have environmental disadvantages. This study investigates the extraction of chitin from Litopenaeus vannamei shrimp waste by one-step fermentation using the bacterial strains Pseudomonas aeruginosa QF50 and Serratia sp. QCS23. A total of 4 kg of shrimp waste was treated by fermentation with culture media enriched with different concentrations of glucose (1, 5, and 10%) for 7 days at 25 °C, followed by purification and characterization processes using infrared spectroscopy and X-ray diffraction. The results demonstrated an increase in the yield of crude chitin proportional to the glucose concentration, reaching a maximum of 76.81 ± 7.64% for Pseudomonas aeruginosa QF50 and 71.30 ± 1.16% for Serratia sp. QCS23. Both strains showed high efficiencies in deproteinization (80–87%) and demineralization, with significant improvements especially shown at high glucose concentrations. Structural characterization confirmed the presence of the spectral characteristics of α-chitin, with crystallinity indices of 81% and 71% for chitins obtained with Pseudomonas aeruginosa QF50 and Serratia sp. QCS23, respectively. This study concludes that single-step fermentation with Pseudomonas aeruginosa QF50 and Serratia sp. QCS23 is an effective and sustainable method for the extraction of high-quality chitin from shrimp exoskeleton waste, offering a promising alternative to traditional chemical methods.

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Section: Resultsmentioning

confidence: 88%