Microbial Colonization and their Relation with Potential Cofactors in Patients with Denture Stomatitis

Abstract

Aim To determine microbial prevalence in the mucous membrane and prosthesis of denture wearers with and without denture stomatitis and to study its relationship with potential clinical cofactors. Materials and methods Saliva was collected from 200 patients (100 female and 100 male) wearing dental prosthesis for measurement of pH. Oral samples of the mucous membrane and of dentures were taken with sterile swab for microbiological analysis. Medical and dietary history of the subjects was recorded. Chi-square test and analysis of varience were used for statistical analysis. Results Diabetes, hypertension and high carbohydrate ingestion was observed in majority of patients. The pH average in saliva was of 5.1. The presence of C. albicans, S. aureus and S. mutans in the mucous membrane was of 55.4, 56.4 and 63.6% respectively. C. albicans was isolated in 69.7% from the prosthesis, whereas S. aureus and S. mutans were isolated in 45.3%. The isolation of C.albicans was more frequent in patients with deture stomatitis. Conclusion C. albicans, S. aureus and S. mutans frequently colonize the oral mucous of denture wearers. This is more frequently observed in patients with denture stomatitis. Clinical significance Denture stomatitis is associated to Candida albicans, different bacteria and other cofactors, such as salivary pH, carbohydrate ingestion, systemic illnesses and medication. How to cite this article Chopde N, Jawale B, Pharande A, Chaudhari L, Hiremath V, Redasani R. Microbial Colonization and their Relation with Potential Cofactors in Patients with Denture Stomatitis. J Contemp Dent Pract 2012;13(4):456-459.

Immune Response and Candidal Colonisation in Denture Associated Stomatitis

“…In such studies, C. albicans is reported as being the most prevalent Candida species on the dentures of stomatitis patients, with an incidence of 54-75%, and a lower incidence evident on the dentures on non DS patients (39%) [28][29][30]. In the present study, similar findings were reported, with a 57% incidence of oral colonisation by C. albicans in DS patients and a 22% incidence in non-DS patients.…”

“…A number of previous investigations have examined the microbiology of DS, particularly with respect to the Candida species colonisation of the oral cavity [28][29][30]. In such studies, C. albicans is reported as being the most prevalent Candida species on the dentures of stomatitis patients, with an incidence of 54-75%, and a lower incidence evident on the dentures on non DS patients (39%) [28][29][30].…”

Host’s Immunity and Candida Species Associated with Denture Stomatitis: A Narrative Review

“…Thus, several pathologies and their treatments can increase a patient’s exposure to C. albicans infection [ 156 ]. Frequently the atrophic aspect of denture stomatitis appears to be related to a general pathology such as diabetes and hypertension [ 157 ].…”

An organotypic oral mucosal infection model to study host-pathogen interactions

“…S.aureus is now an accepted member of the normal oral flora and has been detected in conjunction with C.albicans in the oral cavity, and is thought to be associated with DS . [10][11][12] More recently, small colony variants of S.aureus were found to associate with C.albicans amongst denture wearers. 13 Elsewhere, S.aureus infections range from superficial, localised wound infections to systemic sepsis, all of which are dependent on a portal of entry through the epithelial barrier.…”