Microbial colonization of different microplastic types and biotransformation of sorbed PCBs by a marine anaerobic bacterial community

Rosato, Antonella; Barone, Monica; Negroni, Andrea; Brigidi, Patrizia; Fava, Fabio; Xu, Ping; Candela, Marco; Zanaroli, Giulio

doi:10.1016/j.scitotenv.2019.135790

Cited by 100 publications

(32 citation statements)

References 63 publications

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“…2 and 3). Studies that were clearly less similar to other studies could be explained by them being laboratory-based [32,71], focussed on anaerobic rather than aerobic communities [71], collecting samples from the deep sea [76] or sequencing amplicons that did not include the V4 16S rRNA gene region [52,71]. Those that were particularly similar within [26] or between [72][73][74] studies could presumably be explained by very long incubation times (above 1 year), leading to community convergence, or having similar experimental setups and inoculums, respectively (Fig.…”

Section: Diversity Within and Between Studiesmentioning

confidence: 76%

Food or just a free ride? A meta-analysis reveals the global diversity of the Plastisphere

2020

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It is now indisputable that plastics are ubiquitous and problematic in ecosystems globally. Many suggestions have been made about the role that biofilms colonizing plastics in the environment—termed the “Plastisphere”—may play in the transportation and ecological impact of these plastics. By collecting and re-analyzing all raw 16S rRNA gene sequencing and metadata from 2,229 samples within 35 studies, we have performed the first meta-analysis of the Plastisphere in marine, freshwater, other aquatic (e.g., brackish or aquaculture) and terrestrial environments. We show that random forest models can be trained to differentiate between groupings of environmental factors as well as aspects of study design, but—crucially—also between plastics when compared with control biofilms and between different plastic types and community successional stages. Our meta-analysis confirms that potentially biodegrading Plastisphere members, the hydrocarbonoclastic Oceanospirillales and Alteromonadales are consistently more abundant in plastic than control biofilm samples across multiple studies and environments. This indicates the predilection of these organisms for plastics and confirms the urgent need for their ability to biodegrade plastics to be comprehensively tested. We also identified key knowledge gaps that should be addressed by future studies.

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Section: Diversity Within and Between Studiesmentioning

confidence: 76%

Food or just a free ride? A meta-analysis reveals the global diversity of the Plastisphere

2020

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“…8). We also detected enrichment of Lentisphaerae and Dehalococcoidia lineages, which are associated with polychlorinated biphenyl (PCB) contamination (Matturro et al 2017, Rosato et al 2020. In coastal sediments (sediment surface only), we found enrichment of photosynthetic lineages, including the oxygenic Cyanobacteriales and anoxygenic Chromatiales, which may co-exist due to a rapid depletion of oxygen at the sediment surface ( Supplementary Fig.…”

Section: Figurementioning

confidence: 83%

Diversity, activity and abundance of benthic microbes in the southeastern Mediterranean Sea: A baseline for monitoring

Rubin‐Blum

Sisma-Ventura

Yudkovski

et al. 2021

Preprint

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Microbes are key players in marine sediments, yet they are not accessed routinely by monitoring programs. Here, we investigate the spatial and vertical trends in the abundance, activity and diversity of benthic archaea, bacteria and fungi of the southeastern Mediterranean Sea (SEMS), based on ∼150 samples collected by the National Monitoring Program in 2018-2020 in coastal, as well as deep-sea transects across the Israeli exclusive economic zone, using vertical profiles of short sediment cores (0-1, 1-2, 4-5, 9-10 and 19-20 cm below surface). Microbial abundance was usually low (0.01 ×108 to 0.21×108 cells gr-1 sediment), while heterotrophic productivity was the highest in the nearshore stations (12±4 ng C gr-1 sediment h-1), as opposed to 0.5±0.9 ng C gr-1 sediment h-1 at the offshore sites. Using amplicon sequencing of marker genes, we identified the changes in the diversity of microbes along environmental gradients, in the four dimensions (geographic location, seabed depth, distance from the sediment surface and time). We show high taxonomic diversity of bacteria and archaea (Shannon’s H’ 5.0-6.9) and lesser diversity of fungi (Shannon’s H’ 0.2-4.8). We use DESeq2 analyses to highlight the role of ammonia-oxidizing Nitrososphaeria in the aerated sediments of the continental slope and deep bathyal plain stations and organotrophic lineages in coastal, shelf, slope, and abyssal plain sediments. Based on taxonomic diversity, we infer the metabolic potential of these communities. Analyses of fungi diversity and guilds suggest the prevalence of the saprotrophic and pathotrophic microfungi Ascomycota (70±23%) and Basidiomycota (16±18%) in the SEMS sediments. We provide a comprehensive baseline of benthic microbial populations in the SEMS and pledge for the use of microbial indices in biomonitoring of the marine environment.

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“…Microbes have potentials to degrade plastics with ester bond via enzymatic hydrolysis through colonization onto the surfaces of materials 18, 19, 21 . Because some marine bacteria have the ability to degrade hydrocarbons which possessing similar chemical structure with plastics, it has been suggested that a certain fraction of the microbial community colonizing plastics might have capability of degrading plastics 31, 32 and thereby using as a carbon matrix. However, it remains unclear whether and to what extent marine prokaryotic communities are capable of degrading plastic in the ocean.…”

Section: Discussionmentioning

confidence: 99%

A marine bacterial community that degrades poly(ethylene terephthalate) and polyethylene

Gao

Sun

2020

Preprint

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Plastic wastes have become the most common form of marine debris and present a growing global pollution problem. Recently, microorganisms-mediated degradation has become a most promising way to accomplish the eventual bioremediation of plastic wastes due to their prominent degradation potentials. Here, a marine bacterial community which could efficiently colonize and degrade both poly (ethylene terephthalate) (PET) and polyethylene (PE) was discovered through a screening with hundreds of plastic waste associated samples. Using absolute quantitative 16S rRNA sequencing and cultivation methods, we obtained the abundances and pure cultures of three bacteria mediating plastic degradation. We further reconstituted a tailored bacterial community containing above three bacteria and demonstrated its efficient degradation of PET and PE through various techniques. The released products from PET and PE degraded by the reconstituted bacterial community were determined by the liquid chromatography-mass spectrometry. Finally, the plastic degradation process and potential mechanisms mediated by the reconstituted bacterial community were elucidated through transcriptomic methods. Overall, this study establishes a stable and effective marine bacterial community for PET and PE degradation and sheds light on the degradation pathways and associated mechanistic processes, which paves a way to develop a microbial inoculant against plastic wastes.

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Microbial colonization of different microplastic types and biotransformation of sorbed PCBs by a marine anaerobic bacterial community

Cited by 100 publications

References 63 publications

Food or just a free ride? A meta-analysis reveals the global diversity of the Plastisphere

Food or just a free ride? A meta-analysis reveals the global diversity of the Plastisphere

Diversity, activity and abundance of benthic microbes in the southeastern Mediterranean Sea: A baseline for monitoring

A marine bacterial community that degrades poly(ethylene terephthalate) and polyethylene

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