Microbial colonization of metal sulfide minerals at a diffuse‐flow deep‐sea hydrothermal vent at 9°50′N on the East Pacific Rise

Wang, Chloe H.; Gulmann, Lara K.; Zhang, Tong; Farfán, Gabriela; Hansel, Colleen M.; Sievert, Stefan M.

doi:10.1111/gbi.12396

Cited by 3 publications

(3 citation statements)

References 55 publications

(56 reference statements)

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“…The clones in the library of the primary culture were affiliated with the following taxonomic groups ( Figure 2 ): the active bacterial community was mainly composed of Gammaproteobacteria (92.66–98.95%), Alphaproteobacteria (0.004–5.27%), Campylobacteria (0–0.8%), Actinobacteria (0–0.39%), Bacteroidia (0–2.04%), and unclassified proteobacteria . Many of the phylotypes in the primary cultures are similar to the in situ enrichment clones recovered from the sulfide mineral of the active vents ( Wang C. H. et al, 2020 ) and the uncultured environmental clones retrieved from the plumes of the active vents ( Sylvan et al, 2012a ). Simultaneously, these phylotypes were also detected in secondary enrichment cultures at the end of 1 month of long-term incubation ( Figure 2 ).…”

Section: Resultsmentioning

confidence: 70%

Potential autotrophic carbon-fixer and Fe(II)-oxidizer Alcanivorax sp. MM125-6 isolated from Wocan hydrothermal field

et al. 2022

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The genus Alcanivorax is common in various marine environments, including in hydrothermal fields. They were previously recognized as obligate hydrocarbonoclastic bacteria, but their potential for autotrophic carbon fixation and Fe(II)-oxidation remains largely elusive. In this study, an in situ enrichment experiment was performed using a hydrothermal massive sulfide slab deployed 300 m away from the Wocan hydrothermal vent. Furthermore, the biofilms on the surface of the slab were used as an inoculum, with hydrothermal massive sulfide powder from the same vent as an energy source, to enrich the potential iron oxidizer in the laboratory. Three dominant bacterial families, Alcanivoraceae, Pseudomonadaceae, and Rhizobiaceae, were enriched in the medium with hydrothermal massive sulfides. Subsequently, strain Alcanivorax sp. MM125-6 was isolated from the enrichment culture. It belongs to the genus Alcanivorax and is closely related to Alcanivorax profundimaris ST75FaO-1T (98.9% sequence similarity) indicated by a phylogenetic analysis based on 16S rRNA gene sequences. Autotrophic growth experiments on strain MM125-6 revealed that the cell concentrations were increased from an initial 7.5 × 105 cells/ml to 3.13 × 108 cells/ml after 10 days, and that the δ13CVPDB in the cell biomass was also increased from 234.25‰ on day 2 to gradually 345.66 ‰ on day 10. The gradient tube incubation showed that bands of iron oxides and cells formed approximately 1 and 1.5 cm, respectively, below the air-agarose medium interface. In addition, the SEM-EDS data demonstrated that it can also secrete acidic exopolysaccharides and adhere to the surface of sulfide minerals to oxidize Fe(II) with NaHCO3 as the sole carbon source, which accelerates hydrothermal massive sulfide dissolution. These results support the conclusion that strain MM125-6 is capable of autotrophic carbon fixation and Fe(II) oxidization chemoautotrophically. This study expands our understanding of the metabolic versatility of the Alcanivorax genus as well as their important role(s) in coupling hydrothermal massive sulfide weathering and iron and carbon cycles in hydrothermal fields.

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Section: Resultsmentioning

confidence: 70%

Potential autotrophic carbon-fixer and Fe(II)-oxidizer Alcanivorax sp. MM125-6 isolated from Wocan hydrothermal field

et al. 2022

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“…Some minerals containing variable-valence elements can also affect microbial communities by generating energy through terminal electron acceptor or donor redox [ 26 ], especially iron-binding or sulfur-binding minerals [ 27 , 28 ]. Wang et al interpreted the bioavailability of metal sulfide minerals (mercury sulfides, pyrite, and marcasite) as electron donors that may be the key control on deep-sea chemosynthetic community activity and proliferation [ 29 ]. In addition, the influence of minerals on the community assemblage processes was also controlled by external factors (conditions of the bulk environments), such as land-use intensities [ 22 ], hot springs sedimentary composition [ 15 ], and forest plant cover [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

“…In the extreme environment, minerals act as a particular interface and additional selective pressure on microbes, due to their special physical properties and nutrient content [ 14 , 15 ]. Such microbes’ selective colonization of different minerals has been observed in a subglacial environment and a submarine hydrothermal system [ 29 , 33 ]. However, relatively few mineral colonization studies have been done in hot springs [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Mineral on Taxonomic and Functional Structures of Microbial Community in Tengchong Hot Springs via in-situ cultivation

Hou

Wang

et al. 2023

Environmental Microbiome

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Diverse mineralogical compositions occur in hot spring sediments, but the impact of minerals on the diversity and structure of microbial communities remains poorly elucidated. In this study, different mineral particles with various chemistries (i.e., hematite, biotite, K-feldspar, quartz, muscovite, aragonite, serpentine, olivine, barite, apatite, and pyrite) were incubated for ten days in two Tengchong hot springs, one alkaline (pH ~ 8.34) with a high temperature (~ 82.8 °C) (Gumingquan, short as GMQ) and one acidic (pH ~ 3.63) with a relatively low temperature (~ 43.3 °C) (Wenguangting, short as WGT), to determine the impacts of minerals on the microbial communities taxonomic and functional diversities. Results showed that the mineral-associated bacterial taxa differed from those of the bulk sediment samples in the two hot springs. The relative abundance of Proteobacteria, Euryarchaeota, and Acidobacteria increased in all minerals, indicating that these microorganisms are apt to colonize on solid surfaces. The α-diversity indices of the microbial communities on the mineral surfaces in the WGT were higher than those from the bulk sediment samples (p < 0.05), which may be caused by the stochastically adhering process on the mineral surface during 10-day incubation, different from the microbial community in sediment which has experienced long-term environmental and ecological screening. Chemoheterotrophy increased with minerals incubation, which was high in most cultured minerals (the relative contents were 5.8 − 21.4%). Most notably, the sulfate respiration bacteria (mainly related to Desulfobulbaceae and Syntrophaceae) associated with aragonite in the acidic hot spring significantly differed from other minerals, possibly due to the pH buffering effect of aragonite providing more favorable conditions for their survival and proliferation. By comparison, aragonite cultured in the alkaline hot spring highly enriched denitrifying bacteria and may have promoted the nitrogen cycle within the system. Collectively, we speculated that diverse microbes stochastically adhered on the surface of minerals in the water flows, and the physicochemical properties of minerals drove the enrichment of certain microbial communities and functional groups during the short-term incubation. Taken together, these findings thereby provide novel insights into mechanisms of community assembly and element cycling in the terrestrial hydrothermal system associated with hot springs.

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The interaction of acidophiles driving community functional responses to the re-inoculated chalcopyrite bioleaching process

Huang

et al. 2021

Science of The Total Environment

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Microbial colonization of metal sulfide minerals at a diffuse‐flow deep‐sea hydrothermal vent at 9°50′N on the East Pacific Rise

Abstract: This is the author manuscript accepted for publication and has undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as

Cited by 3 publications

References 55 publications

Potential autotrophic carbon-fixer and Fe(II)-oxidizer Alcanivorax sp. MM125-6 isolated from Wocan hydrothermal field

Potential autotrophic carbon-fixer and Fe(II)-oxidizer Alcanivorax sp. MM125-6 isolated from Wocan hydrothermal field

Effects of Mineral on Taxonomic and Functional Structures of Microbial Community in Tengchong Hot Springs via in-situ cultivation

The interaction of acidophiles driving community functional responses to the re-inoculated chalcopyrite bioleaching process

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