Microbial Communities and Metabolites of Whole Crop Corn Silage Inoculated with Lentilactobacillus plantarum and Lentilactobacillus buchneri

Guo, Qian; Xia, Hao; Li, Yuerui; Zhang, Qing; Wang, Chao; Han, Hairong

doi:10.3390/pr10112369

Cited by 5 publications

(2 citation statements)

References 30 publications

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“…The pH value is an important indicator for assessing the quality of silage, with lower values indicating better ensiling effects. Well-fermented silage should have a pH value of 4.2 or lower [19]. In this study, the CE and PHCE groups had the lowest pH values at 30 days after ensiling, which may be attributed to the favorable environment created by cellulase enzymes for the proliferation of lactic acid bacteria, inhibiting the growth of harmful bacteria and promoting microbial activity and growth in the silage, ensuring successful ensiling.…”

Section: Fermentation Characteristics and Nutrient Composition Of Sor...mentioning

confidence: 67%

WITHDRAWN: Effects of enzyme preparation and straw expansion treatment on sorghum micro-storage performance and microbial community

Sun,

Liu,

Bai

et al. 2024

Preprint

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Background Sorghum is commonly used as feed in livestock farming. However, due to its high lignin and cellulose content, sorghum straw is not highly digestible for animals. To improve the feeding value of sorghum straw, researchers have explored micro-storage techniques. Enzyme preparation and straw expansion treatment are two commonly used methods, but their effects on sorghum micro-storage performance require further investigation.Results Sorghum exhibits relatively low nutritional value, but the addition of enzyme preparation and puffing treatment can enhance its value. These methods facilitate the breakdown of plant cell walls, releasing more nutrients and fermentable sugars, thus improving the digestibility and nutritional value of ensiled feed. Cellulase and xylanase can degrade cellulose and hemicellulose, respectively, which serve as important carbon sources for alkaligenic bacteria growth. Straw expansion treatment alters the proportions of lignin, cellulose, and hemicellulose in sorghum straw, leading to partial lignin degradation and an increase in organic matter content. The presence of lactic acid bacteria and alkaligenic bacteria enhances carbohydrate metabolism, amino acid, coenzyme, and vitamin metabolism, as well as signal transduction pathways, thereby improving microbial community structure, metabolism, and fermentation quality of ensiled sorghum feed. This study found that combined xylanase addition after expansion treatment significantly improves the micro-storage performance of sorghum straw. These findings provide a theoretical basis for optimizing sorghum straw micro-storage techniques to enhance its feeding value and animal production performance.Conclusions This study demonstrated that the addition of enzyme preparation significantly improved the quality of ensiled sorghum straw, with the best results achieved through the addition of xylanase. The sequential application of expansion treatment and enzyme addition yielded the best micro-storage performance for sorghum straw. These findings provide a theoretical basis for optimizing sorghum straw micro-storage techniques, contributing to increased feeding value and animal production performance. This study revealed, for the first time, the underlying principles of the synergistic effect between enzyme preparation and straw expansion treatment in the sorghum ensiling system, which provided valuable insights for the development and utilization of sorghum feed resources, and promotion of sustainable livestock production.

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Section: Fermentation Characteristics and Nutrient Composition Of Sor...mentioning

confidence: 67%

WITHDRAWN: Effects of enzyme preparation and straw expansion treatment on sorghum micro-storage performance and microbial community

Sun,

Liu,

Bai

et al. 2024

Preprint

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“…Metabolites of mulberry silage-inoculated LAB strains were at a distance from the control group ( Figure 5 ), suggesting that inoculated L. plantarum or P. pentosaceus influenced metabolite compositions of mulberry silage by the microbial community. This finding was consistent with the findings of a previous study (Guo et al, 2022 ). LAB could convert and degrade phenolic compounds by various enzymes such as tannase, β-glucosidase, and phenolic acid decarboxylase (De Montijo-Prieto et al, 2023 ).…”

Section: Discussionsupporting

confidence: 94%

Chemical characteristics, antioxidant capacity, bacterial community, and metabolite composition of mulberry silage ensiling with lactic acid bacteria

Guo,

Huang,

Niu

et al. 2024

Front. Microbiol.

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Mulberry has high crude protein and biologically active compounds but is difficult to be ensiled due to the lack of adequate epiphytic LAB. This study aimed to investigate the effects of inoculation with Lactiplantibacillus plantarum and Pediococcus pentosaceus isolated from mulberry with higher antioxidant capacity alone or in combination with Streptococcus bovis on chemical characteristics, antioxidant capacity, bacterial community, and metabolite composition of mulberry silage. The results showed that all inoculation groups had higher dry matter and lower pH than the control group, particularly in LP (dry matter, DM, 32.03% and pH = 4.44) and LP_PP_SB (DM, 31.68% and pH = 4.26) after 60 days of ensiling. Ammonia nitrogen (AN) content was the lowest in both LP_SB and LP_PP_SB groups, which were 1.86 g/kg FM and 1.05 g/kg FM, respectively, (P < 0.05). Only the LP_PP_SB group showed increased polyunsaturated fatty acids (PUFA, 1.2851 g/kg DM, P < 0.05) than the control group. Ferric-reducing antioxidant power (FRAP) values were increased in all inoculation-treated groups compared with the control group (P < 0.05). 2,2-Diphenyl-1-picrylhydrazyl (DDPH), 3-ethylbenzothiazoline-6-sulfonic acid (ABTS), and FRAP exhibited the highest levels in the LP_PP- and LP_PP_SB-treated groups. Enterobacter was dominant in both the control and SB-treated groups, and the relative abundance was 41.18% and 32.35%, respectively (P < 0.05). The relative abundance of Lactiplantibacillus was higher in the LP-, LP_PP-, and LP_SB-treated groups (81.84%−82.69%). Relative abundance of Pediococcus was higher in the PP-, PP_SB-, and LP_PP_SB-treated groups (74.27%−85.27%). Untargeted metabolomics analysis results showed that five flavonoids (apigenin, eriodictyol, quercetin-3-glucoside, rutin, and kaempferol-3-O-rutinoside)were upregulated in all inoculation groups (except for the SB-treated groups). Among them, eriodictyol was both positively correlated with ABTS and FRAP and also showed the highest relative abundance in the LP_PP- and LP_PP_SB-treated groups. To the best of our knowledge, this study was the first to investigate the relationship between inoculants of epiphytic lactic acid bacteria and antioxidant capacity by 16s rRNA Illumina sequencing technology and untargeted metabolomics analysis, respectively. Consequently, inoculated L. plantarum, P. pentosaceus alone, respectively, or in combination with S. bovis increased the relative abundance of Lactiplantibacillus and Pediococcus and decreased the relative abundance of Enterobacter, particularly in the LP_PP_SB-treated group. In addition, inoculants could increase the relative abundance of five flavonoids (apigenin, eriodictyol, quercetin-3-glucoside, rutin, and kaempferol-3-O-rutinoside), especially eriodictyol to improve the antioxidant capacity of mulberry silage.

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Effects of lactic acid bacteria and cellulase additives on the fermentation quality, antioxidant activity, and metabolic profile of oat silage

Wang,

Liu,

Wang

et al. 2024

Bioresour. Bioprocess.

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Oats (Avena sativa L.) are rich in nutrients and bioactive compounds, serving as a roughage source for ruminants. This study investigated the impact of lactic acid bacteria (LAB), cellulase (M), and their combinations (LM) on the fermentation quality and metabolic compounds of oat silage. Results demonstrated that all additive treatments significantly increased lactic acid content compared to the control group (P < 0.05), with the lactic acid bacteria treatment group exhibiting the lowest pH value (P < 0.05). Analysis of antioxidant activity and metabolites in oat silage over 60 days revealed 374 differential metabolites with 113 up-regulated and 261 down-regulated, and all treatment groups showing higher antioxidant activity than raw oat materials (P < 0.05). Although no significant differences in antioxidant activity were observed among the various treatment groups in this experiment, notable changes in metabolic pathways were identified. Furthermore, two metabolites (carboxylic acids and derivatives and benzene and substituted derivatives) were identified through non-targeted metabolomics technology, both of which are strongly associated with the antioxidant activity of oat silage. This finding provides a theoretical basis for the efficient use of oat silage in animal husbandry. Graphical abstract

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Microbial Communities and Metabolites of Whole Crop Corn Silage Inoculated with Lentilactobacillus plantarum and Lentilactobacillus buchneri

Cited by 5 publications

References 30 publications

WITHDRAWN: Effects of enzyme preparation and straw expansion treatment on sorghum micro-storage performance and microbial community

WITHDRAWN: Effects of enzyme preparation and straw expansion treatment on sorghum micro-storage performance and microbial community

Chemical characteristics, antioxidant capacity, bacterial community, and metabolite composition of mulberry silage ensiling with lactic acid bacteria

Effects of lactic acid bacteria and cellulase additives on the fermentation quality, antioxidant activity, and metabolic profile of oat silage

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