Microbial communities associated with the root system of wild olives (Olea europaea L. subsp. europaea var. sylvestris) are good reservoirs of bacteria with antagonistic potential against Verticillium dahliae

Soil factors involved in the diversity and structure of soil bacterial communities in commercial organic olive orchards in Southern Spain

“…T‐RFLP analysis of amplified 16S rDNA was performed as described in Aranda and colleagues (). Briefly, the bulk community of DNA was extracted from 500 mg of soil samples (three replicates per farm or NV area) using the MoBio Ultraclean soil DNA isolation kit (MoBio Laboratories; Carlsbad, CA, USA).…”

“…Briefly, the bulk community of DNA was extracted from 500 mg of soil samples (three replicates per farm or NV area) using the MoBio Ultraclean soil DNA isolation kit (MoBio Laboratories; Carlsbad, CA, USA). Then, 16S rDNA sequences were amplified using primers 8f (5′ end‐labelled with the fluorescent dye 6‐carboxy‐ fluorescein FAM) and 1492r as described before, and 5 μl of polymerase chain reaction products were digested using Msp I or Rsa I enzymes (Fast Digest, Fermentas, St. Leon‐Rot, Germany) in a final volume of 10 μl according to Aranda and colleagues (). TRFs were loaded and separated on a 3130XL genetic analyzer (Applied Biosystems, Foster City, CA, USA) at the SCAI‐University of Córdoba sequencing facilities.…”

“…Size of fragments and matrices containing incidence as well as peak area data of individual TRFs were generated for all soil samples using GeneMapper software (Applied Biosystems). TRFs profiles were selected, standardized and combined for the three replications based on methods described previously in Aranda and colleagues ().…”

“…1). d. Diversity statistics were calculated from standardized profiles of soil samples by using the number and area of peaks in each profile as representative of the number and relative abundance of phylotypes, as described before (Aranda et al, 2011). Phylotype richness (S) was calculated as the total number of distinct TRF sizes (between 50 and 500 bp) in a profile.…”

Agronomic evaluation of seedlings from crosses between the main Spanish olive cultivar ‘Picual’ and two wild olive trees

“…sylvestris) may contain even greater allelic richness (Lumaret et al, 2004) and genetic variability (Baldoni et al, 2006;Belaj et al, 2007;. In fact, wild olive might be an interesting gene pool for use in mainstream olive breeding programmes with objectives such as increasing biotic (Ciccarese et al, 2002;Mkize et al, 2008;Sesli et al, 2010) and/or abiotic (Mulas, 1999;Baldoni et al, 2006;Meddad-Hamza et al, 2010;Aranda et al, 2011) stress resistance, higher productivity and increased fruit setting (Hannachi and Marzouk, 2012), as well as improved oil quality (Sedgley, 2000;Baccaouri et al, 2008;Hannachi et al, 2008). The combined use of cultivated and wild genotypes in olive breeding programmes may increase heterosis in the resulting progeny (Biton et al, 2012).…”