Microbial Communities in Long-Term, Water-Flooded Petroleum Reservoirs with Different in situ Temperatures in the Huabei Oilfield, China

Abstract

The distribution of microbial communities in the Menggulin (MGL) and Ba19 blocks in the Huabei Oilfield, China, were studied based on 16S rRNA gene analysis. The dominant microbes showed obvious block-specific characteristics, and the two blocks had substantially different bacterial and archaeal communities. In the moderate-temperature MGL block, the bacteria were mainly Epsilonproteobacteria and Alphaproteobacteria , and the archaea were methanogens belonging to Methanolinea , Methanothermobacter , Methanosaeta , and Methanocella . However, in the high-temperature Ba19 block, the predominant bacteria were Gammaproteobacteria , and the predominant archaea were Methanothermobacter and Methanosaeta . In spite of shared taxa in the blocks, differences among wells in the same block were obvious, especially for bacterial communities in the MGL block. Compared to the bacterial communities, the archaeal communities were much more conserved within blocks and were not affected by the variation in the bacterial communities.

Bacterial Communities Associated with Subsurface Geochemical Processes in Continental Serpentinite Springs

“…The most common Erysipelothrix tag sequence was 98 to 99% similar over its length (450 bp) to environmental sequences from a terrestrial mud volcano (42) and a terrestrial oil well (43) in China. Interestingly, both of these studies of subsurface habitats also detected Hydrogenophaga.…”

Use of a Hierarchical Oligonucleotide Primer Extension Approach for Multiplexed Relative Abundance Analysis of Methanogens in Anaerobic Digestion Systems

“…were relatively abundant (31.6% to 45.0% of the amplified Archaea 16S rRNA genes) in anaerobic digesters (cluster III sludge samples), and their abundances could be distinctively discriminated from other sources of sludge samples (0.7% to 6.5% of the amplified Archaea 16S rRNA genes, clusters I and II). In addition to reactor ecosystems, this methanogenic archaeon was present in natural environments (47)(48)(49). Its relatively extensive distribution suggests that this novel archaeon plays an important role in various methanogenic ecosystems.…”

Spatial distributions of methoxylated and hydroxylated polybrominated diphenyl ethers in the East China Sea—A seaward increasing trend

“…DNA from the soil samples were subjected to both bacterial and archaeal T-RFLP analyses as previously described (Yu et al, 2010;Tang et al, 2012). Archaeal and bacterial small subunit ribosomal RNA (SSU rRNA) genes were amplified for T-RFLP analysis using the universal archaeal primers Arch109F (5 -ACK GCT CAG TAA CAC GT-3 ) and 5 6-carboxyfluorescein (FAM)-labeled Arch915R primer (5 -GTG CTC CCC CGC CAA TTC CT-3 ) (Lueders and Friedrich, 2003), and the universal bacterial primers 5 FAMlabeled 8F (5 -AGA GTT TGA TCC TGG CTC AG-3 ) and 1492R (5 -CGG TTA CCT TGT TAC GAC TT-3 ) (Dojka et al, 1998), respectively.…”