Microbial Communities of Stored Product Mites: Variation by Species and Population

Hubert, Jan; Nešvorná, Marta; Green, Stefan J.; Klimov, Pavel B.

doi:10.1007/s00248-020-01581-y

Cited by 20 publications

(18 citation statements)

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“…The genus Staphylococcu s is ubiquitous and is frequently detected in mites, e.g., Psoroptes ovis ( Hogg and Lehane, 1999 ; Oliveira et al, 2006 ) and Demodex folliculorum ( Clifford and Fulk, 1990 ). Staphylococcus were present in most fecal microbiomes of various A. siro , Carpoglyphus lactis and T. putrescentiae cultures ( Hubert et al, 2021 ). Bartonella -like (BOtu3) have been previously considered as being derived from the gut and feces of mites (e.g., Valerio et al, 2005 ; Hubert et al, 2021 ), and we confirm these prior findings here.…”

Section: Discussionmentioning

confidence: 99%

“…Staphylococcus were present in most fecal microbiomes of various A. siro , Carpoglyphus lactis and T. putrescentiae cultures ( Hubert et al, 2021 ). Bartonella -like (BOtu3) have been previously considered as being derived from the gut and feces of mites (e.g., Valerio et al, 2005 ; Hubert et al, 2021 ), and we confirm these prior findings here. For the fungal communities, the genera Yamadazyma and Candida were associated with the fecal treatments.…”

Section: Discussionmentioning

confidence: 99%

“…Prior studies using bleach surface cleaning of mite eggs showed that the eggs still contained viable surface associated microflora, due to surface structures ( Kucerova and Stejskal, 2009 ; Hubert et al, 2021 ). For example, the washing of eggs of the striped shield bug ( Graphosoma lineatum ) in the ethanol did not eliminate the surface microbiota.…”

Section: Discussionmentioning

confidence: 99%

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The Negative Effects of Feces-Associated Microorganisms on the Fitness of the Stored Product Mite Tyrophagus putrescentiae

2022

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Feces have been suggested as a major source of microorganisms for recolonization of the gut of stored product mites via coprophagy. The mites can host microorganisms that decrease their fitness, but their transmission is not known. To address the role of fecal microbiota on mite fitness, we performed an experimental study in which the surfaces of mite (Tyrophagus putrescentiae) eggs were sterilized. Mites eggs (15 per experimental box) were then hatched and grown on feedstock with and without feces. These experiments were conducted with four distinct T. putrescentiae populations (5L, 5K, 5N, and 5P), and mite population density after 21 day of cultivation was used to assess mite fitness and the impact of fecal microbiota on fitness. Population density was not affected by the presence of feces in two of the cultures (5L and 5K), while significant effects of feces were observed in the other cultures (5N and 5P). Mite culture microbial communities were analyzed using cultivation-independent next-generation amplicon sequencing of microbial 16S and 18S ribosomal RNA (rRNA) genes in the fitness influenced populations (5N and 5P). Several microbial taxa were associated with fecal treatments and reduced mite fitness, including Staphylococcus and Bartonella-like bacteria, and the fungal genera Yamadazyma, Candida, and Aspergillus. Although coprophagy is the transmission route mites used to obtain beneficial gut bacteria such as Bartonella-like organisms, the results of this study demonstrate that fecal-associated microorganisms can have negative effects on some populations of T. putrescentiae fitness, and this may counteract the positive effects of gut symbiont acquisition.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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The Negative Effects of Feces-Associated Microorganisms on the Fitness of the Stored Product Mite Tyrophagus putrescentiae

2022

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“…The resulting amplicons were then prepared for sequencing with second-stage PCR. During this second stage, Illumina sequencing adapters and sample-specific barcodes were incorporated into amplicons by PCR amplification with a Fluidigm Access Array for Illumina primers as described previously [ 26 ]. The barcoding PCR step and amplicon sequencing were performed at the Genome Research Core, Research Resources Center, University of Illinois (Chicago, IL, USA) on a MiniSeq platform (Illumina, San Diego, CA, USA) employing paired-end 2 × 153 bp reads.…”

Section: Methodsmentioning

confidence: 99%

“…Based on barcode sequencing, the microbiome of A. siro was found to include Bartonella -like, Solitalea -like, Bacillus sp., and Kocuria sp. [ 26 , 27 ]. Previous studies on the related species Rhizoglyphus robini showed that their microbiomes differ among the different cultures of the same species [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

Pesticide residue exposure provides different responses of the microbiomes of distinct cultures of the stored product pest mite Acarus siro

et al. 2022

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Background The contribution of the microbiome to pesticide breakdown in agricultural pests remains unclear. We analyzed the effect of pirimiphos-methyl (PM) on four geographically different cultures of the stored product pest mite Acarus siro (6 L, 6Tu, 6Tk and 6Z) under laboratory experiments. The effect of PM on mite mortality in the impregnated filter paper test was compared. Results The mite sensitivity to PM decreased in the order of 6 L, 6Tu, 6Tk, and 6Z. Then, the mites were cultured on PM residues (0.0125 and 1.25 µg·g−1), and population growth was compared to the control after 21 days of exposure. The comparison showed two situations: (i) increasing population growth for the most sensitive cultures (6 L and 6Tu), and (ii) no effect on mite population growth for tolerant cultures (6Z and 6Tk). The microbiome of mites was analyzed by quantification of 16S DNA copies based on quantitative polymerase chain reaction (qPCR) and by barcode sequencing of the V4 fragment of 16S DNA on samples of 30 individuals from the control and PM residues. The microbiome comprised primarily Solitalea-like organisms in all cultures, except for 6Z, followed by Bacillus, Staphylococcus, and Lactobacillus. The microbiomes of mite cultures did not change with increasing population density. The microbiome of cultures without any differences in population density showed differences in the microbiome composition. A Sodalis-like symbiont replaced Solitalea in the 1.25 µg·g−1 PM in the 6Tk culture. Sodalis and Bacillus prevailed in the microbiomes of PM-treated mites of 6Z culture, while Solitalea was almost absent. Conclusion The results showed that the microbiome of A. siro differs in composition and in response to PM residues in the diet. The results indicate that Sodalis-like symbionts can help recover mites from pesticide-induced stress.

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Microbial vectoring capacity by internal‐ and external‐infesting stored product insects after varying dispersal periods between novel food patches: An underestimated risk

Ponce,

Maille,

Stoll

et al. 2024

Ecology and Evolution

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Understanding the ability of internal‐ and external‐infesting stored product insects to vector microbes is important for estimating the relative risk that insects pose to postharvest commodities as they move between habitat patches and in the landscape. Thus, the aim of the current study was to evaluate and compare the microbial growth in novel food patches at different dispersal periods by different populations of Sitophilus oryzae (e.g., internal‐infesting) and Lasioderma serricorne (e.g., external‐infesting). Adults of both species collected from laboratory colonies or field‐captured populations were either placed immediately in a novel food patch, or given a dispersal period of 24 or 72 h in a sterilized environment before entering a surrogate food patch. Vectored microbes in new food patches were imaged after 3 or 5 days of foraging, and microbial growth was processed using ImageJ while fungal species were identified through sequencing the ITS4/5 ribosomal subunit. We found that increasing dispersal time resulted in multiple‐fold reductions in microbial growth surrogate food patches by L. serricorne but not S. oryzae. This was likely attributable to higher mobility by S. oryzae than L. serricorne. A total of 20 morphospecies were identified from 13 genera among the 59 sequences, with a total of 23% and 16% classified as Aspergillus and Penicillium spp. Our data suggest that there is a persistent risk of microbial contamination by both species, which has important food safety implications at food facilities.

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Microbial Communities of Stored Product Mites: Variation by Species and Population

Cited by 20 publications

References 63 publications

The Negative Effects of Feces-Associated Microorganisms on the Fitness of the Stored Product Mite Tyrophagus putrescentiae

The Negative Effects of Feces-Associated Microorganisms on the Fitness of the Stored Product Mite Tyrophagus putrescentiae

Pesticide residue exposure provides different responses of the microbiomes of distinct cultures of the stored product pest mite Acarus siro

Microbial vectoring capacity by internal‐ and external‐infesting stored product insects after varying dispersal periods between novel food patches: An underestimated risk

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