Microbial communities profiling in intensive care units expose limitations in current sanitary standards

Ribeiro, Lucas F.; Lopes, Érica Mendes; Kishi, Luciano Takeshi; Ribeiro, Laryssa Freitas; Menegüeti, Mayra Gonçalves; Gaspar, Gilberto Gambero; Silva‐Rocha, Rafael; Guazzaroni, María‐Eugenia

doi:10.1101/633404

Cited by 1 publication

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References 81 publications

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“…We elucidated possible effects on plant-associated microbes, such as Pantoea and Methylobacterium, suggesting a potential overlap in resource utilisation for growth (Figure 5B). The prevalence of Methylobacterium across the ISS, known for its resilience in challenging environments, underscores its adaptability to extreme conditions from NASA spacecraft assembly clean rooms [51] to hospital intensive care units (ICUs) [52]. Additionally, our study highlighted the reciprocal influence between E. bugandensis and Klebsiella species, suggesting a potential reduction in the growth rate for Klebsiella (Figure 5B).…”

Section: Discussionmentioning

confidence: 77%

Genomic, Functional, and Metabolic Enhancements in Multidrug-ResistantEnterobacter bugandensisFacilitating its Persistence and Succession in the International Space Station

Sengupta,

Sivabalan,

Singh

et al. 2023

Preprint

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Background: The International Space Station (ISS) stands as a testament to human achievement in space exploration. Within its highly controlled environment, characterized by microgravity, increased CO2 levels, and elevated solar radiation, microorganisms occupy a unique niche. These microbial inhabitants play a significant role in influencing the health and well-being of astronauts on board. One microorganism of particular interest in our study is Enterobacter bugandensis, primarily found in clinical specimens including human gastrointestinal tract, and also reported to exhibit pathogenic traits, leading to a plethora of infections. Results: Distinct from their Earth counterparts, ISS E. bugandensis strains have exhibited resistance mechanisms that categorize them within the ESKAPE pathogen group, a collection of pathogens recognized for their formidable resistance to antimicrobial treatments. During the two-year Microbial Tracking 1 mission, 12 strains of multidrug-resistant E. bugandensis were isolated from various locations within the ISS. We have carried out a comprehensive study to understand the genomic intricacies of ISS-derived E. bugandensis in comparison to terrestrial strains, with a keen focus on those associated with clinical infections. We aimed to unravel the evolutionary trajectories of pivotal genes, especially those contributing to functional adaptations and potential antimicrobial resistance. A hypothesis central to our study was that the singular stresses of the space environment, distinct from any on Earth, could be driving these genomic adaptations. In extending our investigation, we meticulously mapped the prevalence and distribution of E. bugandensis across the ISS over time. This temporal analysis provided insights into the persistence, succession, and potential patterns of colonization of E. bugandensis in space. Furthermore, by leveraging advanced analytical techniques, including metabolic modeling, we delved into the coexisting microbial communities alongside E. bugandensis in the ISS across multiple missions and spatial locations. This exploration revealed intricate microbial interactions, offering a window into the microbial ecosystem dynamics within the ISS. Conclusions: Our comprehensive analysis illuminated not only the ways these interactions sculpt microbial diversity but also the factors that might contribute to the potential dominance and succession of E. bugandensis within the ISS environment. The implications of these findings are twofold. Firstly, they shed light on microbial behavior, adaptation, and evolution in extreme, isolated environments. Secondly, they underscore the need for robust preventive measures, ensuring the health and safety of astronauts by mitigating risks associated with potential pathogenic threats.

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Section: Discussionmentioning

confidence: 77%

Genomic, Functional, and Metabolic Enhancements in Multidrug-ResistantEnterobacter bugandensisFacilitating its Persistence and Succession in the International Space Station

Sengupta,

Sivabalan,

Singh

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

Microbial communities profiling in intensive care units expose limitations in current sanitary standards

Abstract: 23Hospital-associated infections (HAIs) are the leading cause of morbidity and 24 mortality in intensive care units (ICUs) and neonatal intensive care units (NICUs). 25

Cited by 1 publication

References 81 publications

Genomic, Functional, and Metabolic Enhancements in Multidrug-ResistantEnterobacter bugandensisFacilitating its Persistence and Succession in the International Space Station

Genomic, Functional, and Metabolic Enhancements in Multidrug-ResistantEnterobacter bugandensisFacilitating its Persistence and Succession in the International Space Station

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