Microbial community composition of a down-flow hanging sponge (DHS) reactor combined with an up-flow anaerobic sludge blanket (UASB) reactor for the treatment of municipal sewage

Application of Metagenomics in Environmental Anaerobic Technology

“…HTS-based metagenomic approaches have provided deeper and more thorough understandings of the structure of microbial communities involved in bioenergy (e.g., methane, hydrogen) production from large varieties of organic wastes (e.g., sewage sludge, manure, food residues, cellulose, vinasses, agricultural wastes) or high-strength wastewater (e.g., municipal, brewery, leachate, molasses, food and distillery wastewater) in anaerobic digesters of various designs, including Upflow Anaerobic Sludge Bed (UASB) (Kubota et al 2014;Liu et al 2012;Kim et al 2012), Expanded Granule Sludge Bed (Werner et al 2011), Anaerobic Dynamics Membrane Rioreactor (AnDMBR) Ma et al 2013;Smith et al 2013;Xie et al 2014;Yu et al 2014 HTS-based metagenomic approaches support that while the microbial community is mainly composed of Bacteria and Archaea, Bacteria generally dominate over Archaea in terms of both sequence abundance and diversity in full-scale methanogenic bioreactors. Sundberg et al (2013) investigated microbial richness in 21 full-scale mesophilic (35-40 o C) or thermophilic (51-55 o C) biogas digesters treating sewage sludge or co-digested wastes from slaughter houses, restaurants, households, etc.…”

Efficient regulation of elemental sulfur recovery through optimizing working height of upflow anaerobic sludge blanket reactor during denitrifying sulfide removal process

“…Besides loading ratios, reactor configuration or working area would be other factors that largely impact the recovery efficiency. Previously, Kubota et al (2014) and Lu et al (2015) reported that the UASB height would affect both the inner microbial community and biochemical reaction efficiency. However, so far, reaction mechanism involved functional bacteria and migration and transformation roles of S functional bacteria distribution were investigated along with the height of T-UASB and M-UASB reactors to gain a deeper insight of the interplays between bacteria and substance transformation.…”

Spatial changes in carbon and nitrogen stable isotope ratios of sludge and associated organisms in a biological sewage treatment system

“…The DHS reactor is a novel trickling filter using a sponge media as the sludge carrier in which bacteria and macrofauna are present (Kubota et al, 2014;Onodera et al, 2013). The DHS reactor in combination with the UASB has been verified as a reliable and efficient system for industrial and municipal wastewater treatment for over a decade (Machdar et al, 2000;Onodera et al, 2014;Tandukar et al, 2007).…”

“…The DHS reactor in combination with the UASB has been verified as a reliable and efficient system for industrial and municipal wastewater treatment for over a decade (Machdar et al, 2000;Onodera et al, 2014;Tandukar et al, 2007). Because of the plug flow regime, the characteristics of the retained sludge and microbial community structure vary throughout the reactor height (Kubota et al, 2014;Onodera et al, 2013), making the DHS reactor a suitable model for application of stable isotope analysis. The study aimed to reveal the spatial changes in stable isotope ratios within municipal sewage treatment systems.…”