Microbial community dynamics in phyto-thermotherapy baths viewed through next generation sequencing and metabolomics approach

Abstract

Phyto-thermotherapy is a treatment consisting in immersing oneself in baths of self-heating alpine grass, to benefit of the heat and rich aromatic components released by the process. The aim of this study was to characterize the bacterial and fungal diversity of three phyto-thermal baths (PTB) performed in three different months, and to compare the data with the profile of the volatile organic compounds (VOCs) of the process. All the data collected showed that PTBs were structured in two stages: the first three days were characterised by an exponential rise of the temperature, a fast bacterial development, higher microbial diversity and higher concentrations of plant aliphatic hydrocarbons. The second stage was characterised by a stable high temperature, shrinkage of the microbial diversity with a predominance of few bacterial and fungi species and higher concentrations of volatiles of microbial origin. Erwinia was the dominant microbial species during the first stage and probably responsible of the self-heating process. In conclusion, PTBs has shown both similarities with common self-heating processes and important peculiarities such as the absence of pathogenic bacteria and the dominance of plant terpenoids with health characteristics among the VOCs confirming the evidence of beneficial effects in particular in the first three days.

Maximization of red pigment production from Streptomyces sp. LS1 structure elucidation and application as antimicrobial/antifouling against human pathogens and marine microbes

“…Also, the osmotic shock was used for liberating the pigmented products, especially just in the case of halophilic archaea bacterial strains as reported by Hagazy et al [ 21 ]. Additionally, many protocols for pigment extraction are usually followed using different solvents like hexane, benzene, chloroform, methanol, acetone, ethanol, or mixed solvent [ 35 ]. However, during this study, successful extraction for LS1 pigment was observed by using the organic solvent ethanol with a purity of 99.9% (at a volume ratio 1:1).…”

Bacterial Complexity of Traditional Mountain Butter Is Affected by the Malga-Farm of Production

“…Its relative abundance was always <1% with the exception of Mfarm N, in which it was the dominating taxa and driver characterizing the TMB samples (relative abundance of 23.1%). Erwinia are herb-associated bacteria found in high relative abundances in some Alpine herbs in the Trentino highland in the summer season [43] and can, thus, be introduced into raw milk by a cross-contamination of pasture herbs microbiota. Leuconostoc (driver at Mfarm C) and Streptococcus (driver at both Mfarms B and O) are the most common LAB genera found in milk and dairy products besides Lactococcus [2].…”

Engineered extracellular vesicles as intelligent nanosystems for next-generation nanomedicine

“…269 Studies have found that the intervention of external stimulation in the regulation of body temperature can change the physiological process of the body and even realize the treatment of diseases. 119,270–272 Local hyperthermia has become a powerful tool for treating solid tumors. 273–277 During treatment, tumor tissue is heated to over 50 °C, which initiates a strong cytotoxic effect and causes tumor cell necrosis.…”