Microbial community in persistent apical periodontitis: a 16S r<scp>RNA</scp> gene clone library analysis

Zakaria, Myrna Nurlatifah; Takeshita, Toru; Shibata, Yukie; Maeda, Hidefumi; Wada, Naohisa; Akamine, Akifumi; Yamashita, Yoshihisa

doi:10.1111/iej.12361

Cited by 39 publications

(42 citation statements)

References 47 publications

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“…Enterococcus which has been found to be the most frequently isolated microorganism in root-filled teeth with periapical lesions was detected in a notably low abundance. This finding is in agreement with the results of previous studies using pyrosequencing and gene clone library analysis (14,15,32,33) suggesting probably a previous overestimation of its role in treatment failure.…”

Section: Discussionsupporting

confidence: 93%

“…Our findings, especially from GG database, among the examined group of Greek patients coupled with previously reported evidence from pyrosequencing (7,9–15) and molecular broad-range studies (19,32,33) suggest a high bacterial diversity of endodontic infections. Also, our finding of higher bacterial diversity among persistent versus primary endodontic infections is a novel one, demonstrating that persistent endodontic infections are polymicrobial infections and not caused by a single or few pathogens.…”

Section: Discussionsupporting

confidence: 84%

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Comparison of Bacterial Community Composition of Primary and Persistent Endodontic Infections Using Pyrosequencing

Tzanetakis

Azcarate‐Peril

Zachaki

et al. 2015

Journal of Endodontics

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Introduction Elucidating the microbial ecology of endodontic infections (EI) is a necessary step in developing effective intra-canal antimicrobials. The aim of the present study was to investigate the bacterial composition of symptomatic and asymptomatic primary and persistent infections in a Greek population, using high throughput sequencing methods. Methods 16S amplicon pyrosequencing of 48 root canal bacterial samples was conducted and sequencing data were analyzed using an oral microbiome-specific (HOMD) and a generic (Greengenes; GG) database. Bacterial abundance and diversity were examined by EI type (primary or persistent) and statistical analysis was performed by using non-parametric and parametric tests accounting for clustered data. Results Bacteroidetes was the most abundant phylum in both infection groups. Significant, albeit weak associations of bacterial diversity were found, as measured by UniFrac distances with infection type (ANOSIM R=0.087, P=0.005) and symptoms (ANOSIM R=0.055, P=0.047). Persistent infections were significantly enriched for Proteobacteria and Tenericutes as compared to primary ones; at the genus level, significant differences were noted for 14 taxa, including increased enrichment of persistent infections for Lactobacillus, Streptococcus, and Sphingomonas. More but less-abundant phyla were identified using the GG database; among those, Cyanobacteria (0.018%) and Acidobacteria (0.007%) were significantly enriched among persistent infections. Persistent infections showed higher Phylogenetic Diversity (asymptomatic: PD=9.2, [standard error (se)=1.3]; symptomatic: PD=8.2, se=0.7) compared to primary infections (asymptomatic: PD=5.9, se=0.8; symptomatic: PD=7.4 se=1.0). Conclusions The present study revealed a high bacterial diversity of EI and suggests that persistent infections may have more diverse bacterial communities than primary infections.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 84%

Comparison of Bacterial Community Composition of Primary and Persistent Endodontic Infections Using Pyrosequencing

Tzanetakis

Azcarate‐Peril

Zachaki

et al. 2015

Journal of Endodontics

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“…, Zakaria et al . ). These pathogens have been considered opportunistic or sampling artefacts due to contaminations during laboratory procedures; however, mounting evidence of their presence and role in endodontic disease is arising (Rôças et al .…”

Section: Discussionmentioning

confidence: 97%

Synergistic effect of 2% chlorhexidine combined with proteolytic enzymes on biofilm disruption and killing

et al. 2015

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“…Such complexity in membership makes it difficult to distinguish the roles of specific bacterial species during health and disease. Nevertheless, certain bacteria are overrepresented at specific sites during various pathologies of the oral cavity (8–10). For example, the bacterial genera Prevotella , Porphyromonas and Fusobacterium tend to be overrepresented in infections of the pulp (7, 10).…”

mentioning

confidence: 99%

“…Nevertheless, certain bacteria are overrepresented at specific sites during various pathologies of the oral cavity (8–10). For example, the bacterial genera Prevotella , Porphyromonas and Fusobacterium tend to be overrepresented in infections of the pulp (7, 10). Slight increases of Prevotella on failed implants with respect to healthy teeth have been detected (9).…”

mentioning

confidence: 99%

Bacterial communities associated with apical periodontitis and dental implant failure

Dingsdag

Nelson

Coleman

2016

Microbial Ecology in Health & Disease

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BackgroundPreviously, we demonstrated that bacteria reside in apparently healed alveolar bone, using culture and Sanger sequencing techniques. Bacteria in apparently healed alveolar bone may have a role in peri-implantitis and dental implant failure.ObjectiveTo compare bacterial communities associated with apical periodontitis, those colonising a failed implant and alveolar bone with reference biofilm samples from healthy teeth.Methods and resultsThe study consisted of 196 samples collected from 40 patients undergoing routine dental implant insertion or rehabilitation. The bacterial 16S ribosomal DNA sequences were amplified. Samples yielding sufficient polymerase chain reaction product for further molecular analyses were subjected to terminal restriction fragment length polymorphism (T-RFLP; 31 samples) and next generation DNA sequencing (454 GS FLX Titanium; 8 samples). T-RFLP analysis revealed that the bacterial communities in diseased tissues were more similar to each other (p<0.049) than those from the healthy reference samples. Next generation sequencing detected 13 bacterial phyla and 373 putative bacterial species, revealing an increased abundance of Gram-negative [Prevotella, Fusobacterium (p<0.004), Treponema, Veillonellaceae, TG5 (Synergistetes)] bacteria and a decreased abundance of Gram-positive [(Actinomyces, Corynebacterium (p<0.008)] bacteria in the diseased tissue samples (n=5) relative to reference supragingival healthy samples (n=3).ConclusionIncreased abundances of Prevotella, Fusobacterium and TG5 (Synergistetes) were associated with apical periodontitis and a failed implant. A larger sample set is needed to confirm these trends and to better define the processes of bacterial pathogenesis in implant failure and apical periodontitis. The application of combined culture-based, microscopic and molecular technique-based approaches is suggested for future studies.

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Microbial community in persistent apical periodontitis: a 16S rRNA gene clone library analysis

Cited by 39 publications

References 47 publications

Comparison of Bacterial Community Composition of Primary and Persistent Endodontic Infections Using Pyrosequencing

Comparison of Bacterial Community Composition of Primary and Persistent Endodontic Infections Using Pyrosequencing

Synergistic effect of 2% chlorhexidine combined with proteolytic enzymes on biofilm disruption and killing

Bacterial communities associated with apical periodontitis and dental implant failure

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