Microbial community of freshwater sponges in Lake Baikal

Парфенова, В. В.; Terkina, I. A.; Kostornova, T. Ya.; Nikulina, I. G.; Черных, В. И.; Maksimova, E. A.

doi:10.1134/s1062359008040079

Cited by 22 publications

(15 citation statements)

References 8 publications

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“…This phylum is notable with respect to its abundant and widespread occurrence in various ecosystems and the ability to produce diverse natural products, especially antibiotics, cytotoxic compounds and therapeutics [19]. Besides its recent registration in freshwater sponges [4,25,45], the phylum Actinobacteria is well known for its occurrence [8,15] and potential for secondary metabolite production in marine sponges [18,21]. A recent study on actinobacterial species isolated from the marine sponge Halichondria panicea, for instance, clearly demonstrated the vast repertoire of bioactive metabolites that these organisms produce [20].…”

Section: Discussionmentioning

confidence: 99%

“…phylogeny [49,50], and alternative protocols for the analysis of their diversity and function have been suggested [51][52][53]. Because of their well-reported secondary metabolite production capacity in terrestrial ecosystems [24,54,55], typical symbiotic lifestyle, amenability to cultivation and documented retrieval from freshwater sponges [45], further studies combining alternative molecular tools and culturing might help in gaining novel insights into the occurrence, degree of specificity and potential roles of Pseudomonas species in Spongiliidae.…”

Section: Discussionmentioning

confidence: 99%

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Evidence for Selective Bacterial Community Structuring in the Freshwater Sponge Ephydatia fluviatilis

et al. 2012

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To understand the functioning of sponges, knowledge of the structure of their associated microbial communities is necessary. However, our perception of sponge-associated microbiomes remains mainly restricted to marine ecosystems. Here, we report on the molecular diversity and composition of bacteria in the freshwater sponge Ephydatia fluviatilis inhabiting the artificial lake Vinkeveense Plassen, Utrecht, The Netherlands. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) fingerprints revealed that the apparent diversities within the domain Bacteria and the phylum Actinobacteria were lower in E. fluviatilis than in bulk water. Enrichment of specific PCR-DGGE bands in E. fluviatilis was detected. Furthermore, sponge- and bulk water-derived bacterial clone libraries differed with respect to bacterial community composition at the phylum level. E. fluviatilis-derived sequences were affiliated with six recognized phyla, i.e., Proteobacteria, Planctomycetes, Actinobacteria, Bacteroidetes, Chlamydiae and Verrucomicrobia, in order of relative abundance; next to the uncultured candidate phylum TM7 and one deeply rooted bacterial lineage of undefined taxonomy (BLUT). Actinobacteria, Proteobacteria, and Bacteroidetes were the dominant bacterial phyla in the freshwater clone library whereas sequences affiliated with Planctomycetes, Verrucomicrobia, Acidobacteria and Armatimonadetes were found at lower frequencies. Fine-tuned phylogenetic inference showed no or negligible overlaps between the E. fluviatilis and water-derived phylotypes within bacterial taxa such as Alphaproteobacteria, Bacteroidetes and Actinobacteria. We also ascertained the status of two alphaproteobacterial lineages as freshwater sponge-specific phylogenetic clusters, and report on high distinctiveness of other E. fluviatilis specific phylotypes, especially within the Bacteroidetes, Planctomycetes and Chlamydia taxa. This study supports the contention that the composition and diversity of bacteria in E. fluviatilis is partially driven by the host organism.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Evidence for Selective Bacterial Community Structuring in the Freshwater Sponge Ephydatia fluviatilis

et al. 2012

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“…Taylor et al 2007;Zhu et al 2008;Webster and Blackall 2009;Isaacs et al 2009;Schneemann et al 2010). In contrast there are few studies concerning the diversity of bacteria associated with freshwater sponges (Gernert et al 2005;Parfenova et al 2008). This group of sponges consists of 150 species, which occupy a wide range of freshwater habitats (lakes, rives, streams, fjords, garden ponds) (Manconi and Pronzato 2002).…”

Section: Introductionmentioning

confidence: 94%

Phylogenetic diversity of bacteria associated with the endemic freshwater sponge Lubomirskia baicalensis

Kaluzhnaya

Ицкович

McCormack

2011

World J Microbiol Biotechnol

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In this study, for the first time the diversity of bacteria associated with the endemic freshwater sponge Lubomirskia baicalensis collected from the Sousern Basin of Lake Baikal was investigated employing cultivationindependent approaches. In total, 102 bacterial 16S rRNA clones were screened using restriction fragment length polymorphism (RFLP) and 30 were selected for sequencing. BLASTN and phylogenetic analysis based on near full length 16S rDNA sequences showed that 22 operational taxonomic units (OTUs) were clustered in six known phyla: Actinobacteria (8 OTUs), alpha-Proteobacteria (4 OTUs), beta-Proteobacteria (4 OTUs), Verrucomicrobia (4 OTUs), Nitrospiracea (1 OTU) and Bacteroidetes (1 OTU). Remarkably all phylotypes were affiliated to uncultured microorganisms, however, all alpha-Proteobacteria sequences were closely related to bacteria derived from the freshwater sponge Spongilla lacustris. Our results reveal a high diversity in the L. baicalensis bacterial community and provide an insight into microbial ecology and diversity within freshwater sponges inhabiting the ancient Lake Baikal ecosystem.

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“…The phylum Bacteroidetes, especially the members of Flavobacteria, is highly abundant in the zooplankton-associated community which may play a crucial role in the degradation of high molecular weight organic matter (e.g., cellulose and chitin), proteins, and diatom debris (Qi et al, 2009;Buchan et al, 2014;Yang et al, 2015). The members of Flavobacteria are widely distributed in different host-associated samples, e.g., human gut (Bäckhed et al, 2005), fish gut (Banerjee and Ray, 2017;Egerton et al, 2018), and sponges (Parfenova et al, 2008;Hardoim and Costa, 2014). Another important role of Flavobacteria is that they can degrade cyanotoxins which eventually contribute to release of nutrients in freshwater ecosystems (Macke et al, 2017).…”

Section: Composition Of Microbiomes Of Zooplankton Pa and Bacteriopmentioning

confidence: 99%

Niche Differentiation of Host-Associated Pelagic Microbes and Their Potential Contribution to Biogeochemical Cycling in Artificially Warmed Lakes

et al. 2020

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Microbial community of freshwater sponges in Lake Baikal

Cited by 22 publications

References 8 publications

Evidence for Selective Bacterial Community Structuring in the Freshwater Sponge Ephydatia fluviatilis

Evidence for Selective Bacterial Community Structuring in the Freshwater Sponge Ephydatia fluviatilis

Phylogenetic diversity of bacteria associated with the endemic freshwater sponge Lubomirskia baicalensis

Niche Differentiation of Host-Associated Pelagic Microbes and Their Potential Contribution to Biogeochemical Cycling in Artificially Warmed Lakes

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