Microbial community structure associated with treatment of azo dye in a start-up anaerobic sequenced batch reactor

Yu, Lei; Zhang, Xiaoyu; Shi, Wang; Tang, Qingwen; Xie, Tian; Lei, Ngai-Yu; Chen, Yiliang; Qiao, Weichuan; Li, Wen-Wei; Lam, Michael

doi:10.1016/j.jtice.2015.03.012

Cited by 37 publications

(12 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Because of aeration and SMFC operation, the environmental samples are repaired to a certain extent ( Anawar and Chowdhury, 2020 ; Khan et al, 2021 ). Specialization of the community occurs through the emergence and selection of the most adapted phylotypes, which can be dye-tolerant or decolorizing species ( Yu et al, 2015 ). In polluted environmental samples, bacteria with respiratory versatility are significantly enriched ( Yang et al, 2018 ).…”

Section: Resultsmentioning

confidence: 99%

Function-Oriented Graphene Quantum Dots Probe for Single Cell in situ Sorting of Active Microorganisms in Environmental Samples

et al. 2021

View full text Add to dashboard Cite

Functional microorganisms play a vital role in removing environmental pollutants because of their diverse metabolic capability. Herein, a function-oriented fluorescence resonance energy transfer (FRET)-based graphene quantum dots (GQDs-M) probe was developed for the specific identification and accurate sorting of azo-degrading functional bacteria in the original location of environmental samples for large-scale culturing. First, nitrogen-doped GQDs (GQDs-N) were synthesized using a bottom-up strategy. Then, a GQDs-M probe was synthesized based on bonding FRET-based GQDs-N to an azo dye, methyl red, and the quenched fluorescence was recovered upon cleavage of the azo bond. Bioimaging confirmed the specific recognition capability of GQDs-M upon incubation with the target bacteria or environmental samples. It is suggested that the estimation of environmental functional microbial populations based on bioimaging will be a new method for rapid preliminary assessment of environmental pollution levels. In combination with a visual single-cell sorter, the target bacteria in the environmental samples could be intuitively screened at the single-cell level in 17 bacterial strains, including the positive control Shewanella decolorationis S12, and were isolated from environmental samples. All of these showed an azo degradation function, indicating the high accuracy of the single-cell sorting strategy using the GQDs-M. Furthermore, among the bacteria isolated, two strains of Bacillus pacificus and Bacillus wiedmannii showed double and triple degradation efficiency for methyl red compared to the positive control (strain S12). This strategy will have good application prospects for finding new species or high-activity species of specific functional bacteria.

show abstract

Section: Resultsmentioning

confidence: 99%

Function-Oriented Graphene Quantum Dots Probe for Single Cell in situ Sorting of Active Microorganisms in Environmental Samples

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Recently, more attention has been paid to the anaerobic decolorization of azo dyes for treating industrial wastewater [34]. Compared to the pure culture, the use of mixed microbial consortium in the anaerobic reactor shows efficient decolorization, as it is more capable of handling the heterogeneity of micropollutants in industrial effluents [24,35]. Though the bioreduction of dye and subsequent color removal was quite significant, the process is relatively slow.…”

Section: Introductionmentioning

confidence: 99%

Comparative Anaerobic Decolorization of Azo Dyes by Carbon-Based Membrane Bioreactor

Amin

Giralt

Fortuny

et al. 2021

Water

View full text Add to dashboard Cite

This study used a novel integrated technology of ceramic supported carbon membrane (CSCM) to degrade azo dyes through an anaerobic mixed culture. The CSCM worked simultaneously as biofilm support, redox mediator, and nano-filter to enhance the dye decolorization efficiency. The decolorization of Acid Orange 7 (AO7) was initially investigated with and without microorganisms in both ceramic support (CS) and CSCM reactors. The CSCM bioreactor (B-CSCM), operated with microorganisms, gave a maximum decolorization of 98% using a CSCM evolved from 10% weight (wt.) of Matrimid 5218 solution. To know the influence of permeate flow, feed concentration, and dye structure on the decolorization process, different B-CSCMs for dye removal experiments were studied over monoazo AO7, diazo Reactive Black 5 (RB5), and triazo Direct Blue 71 (DB71). The highest color removal, operated with 50 mg·L−1 feed solution and 0.05 L·m−2·h−1 of permeate flux, was 98%, 82%, and 72%, respectively, for AO7, RB5, and DB71. By increasing these parameters to 100 mg·L−1 and 0.1 L·m−2·h−1, the decolorization rate of dye solution still achieved 37% for AO7, 30% for RB5, and 26% for DB71. In addition, the system was run for weeks without apparent loss of activity. These findings make evident that the combined phenomena taking place in CSCM bioreactor result in an efficient, cost-effective, and ecofriendly azo dye decolorization method.

show abstract

“…But the reduced products of MO, i.e., sulfanilic acid and N,N-dimethyl-p-phenylenediamine (DMPD), could not be further degraded during xylose fermentation. These compounds have been proved recalcitrant to both the pure strain and mixed microorganisms under anaerobic conditions (27)(28)(29)(30). The formation of ethanol, acetate, and hydrogen gas during xylose fermentation suggests that the strain was able to ferment xylose to 3-phosphate-glyceraldehyde via the pentose phosphate pathway (PPP), glycolysis, and biohydrogen pathway in turn, which further proves that the strain belongs to the ethanol/acetate formation type (22).…”

Section: Discussionmentioning

confidence: 89%

Simultaneous Decolorization and Biohydrogen Production from Xylose by Klebsiella oxytoca GS-4-08 in the Presence of Azo Dyes with Sulfonate and Carboxyl Groups

Ming-yue

Wang

et al. 2017

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

Biohydrogen production from the pulp and paper effluent containing rich lignocellulosic material could be achieved by the fermentation process. Xylose, an important hemicellulose hydrolysis product, is used less efficiently as a substrate for biohydrogen production. Moreover, azo dyes are usually added to fabricate anticounterfeiting paper, which further increases the complexity of wastewater. This study reports that xylose could serve as the sole carbon source for a pure culture of Klebsiella oxytoca GS-4-08 to achieve simultaneous decolorization and biohydrogen production. With 2 g liter Ϫ1 of xylose as the substrate, a maximum xylose utilization rate (UR xyl ) and a hydrogen molar yield (HMY) of 93.99% and 0.259 mol of H 2 mol of xylose Ϫ1 , respectively, were obtained. Biohydrogen kinetics and electron equivalent (e Ϫ equiv) balance calculations indicated that methyl red (MR) penetrates and intracellularly inhibits both the pentose phosphate pathway and pyruvate fermentation pathway, while methyl orange (MO) acted independently of the glycolysis and biohydrogen pathway. The data demonstrate that biohydrogen pathways in the presence of azo dyes with sulfonate and carboxyl groups were different, but the azo dyes could be completely reduced during the biohydrogen production period in the presence of MO or MR. The feasibility of hydrogen production from industrial pulp and paper effluent by the strain if the xylose is sufficient was also proved and was not affected by toxic substances which usually exist in such wastewater, except for chlorophenol. This study offers a promising energy-recycling strategy for treating pulp and paper wastewaters, especially for those containing azo dyes.IMPORTANCE The pulp and paper industry is a major industry in many developing countries, and the global market of pulp and paper wastewater treatment is expected to increase by 60% between 2012 and 2020. Such wastewater contains large amounts of refractory contaminants, such as lignin, whose reclamation is considered economically crucial and environmentally friendly. Furthermore, azo dyes are usually added in order to fabricate anticounterfeiting paper, which further increases the complexity of the pulp and paper wastewater. This work may offer a better understanding of biohydrogen production from xylose in the presence of azo dyes and provide a promising energy-recycling method for treating pulp and paper wastewater, especially for those containing azo dyes.KEYWORDS xylose, Klebsiella oxytoca, decolorization, biohydrogen production

show abstract

Microbial community structure associated with treatment of azo dye in a start-up anaerobic sequenced batch reactor

Cited by 37 publications

References 54 publications

Function-Oriented Graphene Quantum Dots Probe for Single Cell in situ Sorting of Active Microorganisms in Environmental Samples

Function-Oriented Graphene Quantum Dots Probe for Single Cell in situ Sorting of Active Microorganisms in Environmental Samples

Comparative Anaerobic Decolorization of Azo Dyes by Carbon-Based Membrane Bioreactor

Simultaneous Decolorization and Biohydrogen Production from Xylose by Klebsiella oxytoca GS-4-08 in the Presence of Azo Dyes with Sulfonate and Carboxyl Groups

Contact Info

Product

Resources

About