Microbial Community Structure of Paddy Soil Under Long-term Fertilizer Treatment Using Phospholipid Fatty Acid (PLFA) Analysis

Abstract

Understanding the microbial community structure of agricultural soils is important for better soil management in order to improve soil quality. Phospholipid fatty acid analysis has been popularly used in determining the microbial community structure in different ecosystems. The microbial community structure of paddy soil under long-term fertilizer treatments was investigated after 45 years using PLFA analysis. Treatments were control (no fertilization, Con), compost (COM), NPK, NPK+compost (NPKC), PK, NK, and NP. Soil chemical properties were mainly affected by the addition of compost and inorganic P fertilizer. Total nitrogen and organic matter contents were significantly higher in treatments with compost while available P 2 O 5 and exchangeable calcium were significantly higher in treatments with added inorganic P fertilizer. It was found that microbial communities were responsive to the different fertilizer treatments. PLFA results showed that the soils were dominated by gram-negative bacteria, followed by the actinomycetes, then gram-positive bacteria, and fungi. Principal component analysis of the soil chemical properties and PLFA composition proved to be a more reliable tool because it was more responsive to the changes in soil chemical properties.

Effects of Long-Term Fertilization on Light and Heavy Fractions of Soil Organic Matter in Single Cropping Paddy Soils in Korea

“…Carbon and nitrogen contents in HF and LF Total carbon and nitrogen contents in plow layer (~15 cm) were significantly affected by fertilization type as shown in Tables 2 and 3 ). These results are in good agreement with the previous results (Lee et al, 2004;Zhang and He, 2004;Gong et al, 2009;Daquiado et al, 2013;Yang et al, 2015). Carbon content in NPK-Com was somewhat higher compared to the mean equilibrium value (about 20 g kg -1 ) reported by Zhang and He (2004).…”

“…The experimental field with different types of fertilization was classified into Pyeongtaeg series (somewhat poorly drained fine silty mixed mesic, Typic Haplaquepts). The detailed experiment design and cultivation background were described by Lee et al (2004) and Daquiado et al (2013). Four plots were selected in this study (see a Table Separation of heavy and light fractions Soil samples were collected from the plow layer (0 -15 cm) in November 2013 after harvest.…”

“…Furthermore, Korean paddy soils undergo alternate turn of summer (wet period) and winter (dry period). In order to evaluate the long-term effects of fertilization patterns on crop yield and soil physicochemical properties in Korea paddy soils, an experimental field has been operating since 1967 at Institute of Crop Science, Milyang (Lee at el., 2004;Daquiado et al, 2013;Lee at el., 2013). The objectives of this study were to 1) investigate the effects of long-term fertilization on both LF and HF of soil organic matter, 2) to elucidate the relationships among total C, C/N ratio, clay content, and fertilization type in paddy soils.…”

Effects of Continuous Application of Green Manures on Microbial Community in Paddy Soil

“…이러한 방법을 통해 무기질 및 유기질비료의 성분별‧시비수준별 토양 미생물군 집분포의 변화에 관한 연구들이 진행되었으며, 국내에서도 과원 및 농경지의 미생물군집분포에 관한 연구결과가 발표 되었다 (Bossio et al, 1998;Peacock et al, 2001;Zhang et al, 2007;Eo et al, 2010;Kim et al, 2015;. 국내 논 토양을 대상으로 한 미생물군집분포에 관한 연구는 무기질비료 장기연용에 의한 영향 및 풋거름보 리, 자운영의 환원에 의한 초기 미생물군집변화에 대하여 주로 이루어졌다 (Daquiado et al, 2013;Kim et al, 2011 (Bossio and Scow, 1998;Daquiado et al, 2013;Eo et al, 2015;Frostegard and Baath, 1996;Kaur et al, 2005;Li et al, 2006;Park and Kim, 2010;Zelles, 1999). Bold-italic factors correspond to the indicators which are considered highly influential on soil microbial community.…”