Microbial community succession in the intestine of mice with deep partial-thickness burns

Abstract

IntroductionBurn injury has been shown to lead to changes in the composition of the gut microbiome and cause other damage in patients. However, little is known about how the gut microbial community evolves in individuals who have recovered from burn injury.MethodsIn this study, we established a model of deep partial-thickness burn in mice and collected fecal samples at eight time points (pre-burn, 1, 3, 5, 7, 14, 21, and 28 days post-burn) for 16S rRNA amplification and high-throughput sequencing.ResultsThe results of the sequencing were analyzed using measures of alpha diversity, and beta diversity and taxonomy. We observed that the richness of the gut microbiome declined from day 7 post-burn and that the principal component and microbial community structure varied over time. On day 28 after the burn, the microbiome composition largely returned to the pre-burn level, although day 5 was a turning point for change. Some probiotics, such as the Lachnospiraceae_NK4A136_group, decreased in composition after the burn but were restored in the later recovery period. In contrast, Proteobacteria showed an opposite trend, which is known to include potential pathogenic bacteria.ConclusionThese findings demonstrate gut microbial dysbiosis after burn injury and provide new insights into the burn-related dysbiosis of the gut microbiome and strategies for improving the treatment of burn injury from the perspective of the microbiota.

In silico analysis of intestinal microbial instability and symptomatic markers in mice during the acute phase of severe burns

“…Notably, analysis of sequencing data from gut microbiota samples at different time points after burn injury revealed that the abundance of the gut microbiota began to decrease from day 7 after burn injury, and its major components and microbial community structure also changed over time. A study from mice showed that by the day 28 after burn injury, the composition of the microbiome had essentially returned to the pre-burn levels [ 9 ]. Similar bacterial diversity was also found between the pre- and post-burn groups in the rat model, but the composition of the gastrointestinal microbiota changed after burns [ 10 ].…”