Microbial consortia for saccharification of woody biomass and ethanol fermentation

Kalyani, Dayanand; Lee, Kyung Min; Kim, Tae Su; Li, Jinglin; Dhiman, Saurabh Sudha; Kang, Yun Chan; Lee, Jung-Kul

doi:10.1016/j.fuel.2013.01.037

Cited by 93 publications

(40 citation statements)

References 34 publications

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“…RCK2010 (214.044 mg/g) and T. reesei RUT-C30 (288 mg/g) [3,29]. The cellulolytic enzymes produced by P. chrysosporium showed higher saccharification efficiency (89.28%) compared to cellulase produced in previous studies by combinations of Agaricus arvensis and Sistotrema brinkmannii (RS, 507 mg/g; 76.7% saccharification yield) and commercial enzymes (Celluclast 1.5 L and Novozyme 188; RS, 429 mg/g; 60% saccharification yield) [23,30].…”

Section: Enzymatic Saccharification Of Lignocellulosic Substrate (Sh)mentioning

confidence: 83%

“…One unit of enzyme activity was defined as the amount of enzyme required to release 1 µmol of reducing sugars (RS) per minute. Cellobiase activity was determined by assaying the release of p-nitrophenol (pNP) using a previously reported procedure [23]. One unit of cellobiase activity was defined as the amount of enzyme required to release 1 µmol of pNP per minute under the assay conditions.…”

Section: Enzyme Assaysmentioning

confidence: 99%

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Fermentative hydrogen production using sorghum husk as a biomass feedstock and process optimization

Saratale

Kshirsagar

Saratale

et al. 2015

Biotechnol Bioproc E

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The potential of isolated actinomycetes and fungi were evaluated for the cellulase and xylanase production under solid state fermentation conditions. Maximal secretion of enzymes was observed with Phanerochaete chrysosporium using soybean straw. The potential of the produced crude enzyme complex was demonstrated by two-step enzymatic hydrolysis of untreated and mild acidpretreated sorghum husk (SH). A cellulase dose of 10 filter paper units (FPU) released 563.21 mg of reducing sugar (RS) per gram of SH with 84.45% hydrolysis and 53.64% glucose yields, respectively. Finally, enzymatic hydrolysates of SH were utilized for hydrogen production by Clostridium beijerinckii. Effects of temperature, pH of media, and substrate concentration on the biohydrogen production from SH hydrolysates were investigated. The optimal conditions for maximal hydrogen production using SH hydrolysate were determined to be a loading of 5.0 g RS/L, at 35°C, and controlled pH at 5.5. Under these optimal conditions, the cumulative H 2 production, H 2 production rate, and H 2 yield were 1,117 mL/L, 46.54 mL/L/h, and 1.051 mol/mol RS, respectively. These results demonstrated a cost-effective hydrogen production is possible with sorghum husk as a lignocellulosic feedstock.

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Section: Enzymatic Saccharification Of Lignocellulosic Substrate (Sh)mentioning

confidence: 83%

Section: Enzyme Assaysmentioning

confidence: 99%

Fermentative hydrogen production using sorghum husk as a biomass feedstock and process optimization

Saratale

Kshirsagar

Saratale

et al. 2015

Biotechnol Bioproc E

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“…Of particular interest is the application of cellulases for the industrial production of bio-ethanol. 24 The realisation of glycosidases' dominant role in many biological and industrial processes has boosted research into the development of techniques to accurately detect and profile these enzymes so as to improve understanding of their function. This review aims to survey the chemical tools developed to detect this important class of enzymes.…”

Section: Thorfinnur Gunnlaugssonmentioning

confidence: 99%

Recent advances in the development of synthetic chemical probes for glycosidase enzymes

2015

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The emergence of synthetic glycoconjugates as chemical probes for the detection of glycosidase enzymes has resulted in the development of a range of useful chemical tools with applications in glycobiology, biotechnology, medical and industrial research. Critical to the function of these probes is the preparation of substrates containing a glycosidic linkage that when activated by a specific enzyme or group of enzymes, irreversibly releases a reporter molecule that can be detected. Starting from the earliest examples of colourimetric probes, increasingly sensitive and sophisticated substrates have been reported. In this review we present an overview of the recent advances in this field, covering an array of strategies including chromogenic and fluorogenic substrates, lanthanide complexes, gels and nanoparticles. The applications of these substrates for the detection of various glycosidases and the scope and limitations for each approach are discussed.

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“…Addition of a mediator has been shown to generate free radicals and it requires additional washing steps, otherwise it will lead to the deactivation of the hydrolytic consortium enzyme (Fillat and Roncero, 2009). 18 Addition of a surfactant not only increased the SY (%) but also provided resistance against the thermal shock and protection against the remaining IPLM in the substrates. Among all of the tested surfactants, only Tween-20 and Tween-80 showed promising results; therefore, further experiments were conducted with only these two surfactants.…”

Section: Simultaneous Pretreatment and Saccharification (Sps)mentioning

confidence: 99%

Simultaneous pretreatment and saccharification: Green technology for enhanced sugar yields from biomass using a fungal consortium

Dhiman

Haw

Kalyani

et al. 2015

Bioresource Technology

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Microbial consortia for saccharification of woody biomass and ethanol fermentation

Cited by 93 publications

References 34 publications

Fermentative hydrogen production using sorghum husk as a biomass feedstock and process optimization

Fermentative hydrogen production using sorghum husk as a biomass feedstock and process optimization

Recent advances in the development of synthetic chemical probes for glycosidase enzymes

Simultaneous pretreatment and saccharification: Green technology for enhanced sugar yields from biomass using a fungal consortium

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