Microbial Consortium of PGPR, Rhizobia and Arbuscular Mycorrhizal Fungus Makes Pea Mutant SGECdt Comparable with Indian Mustard in Cadmium Tolerance and Accumulation

Self Cite

Aluminium being one of the most abundant elements is very toxic for plants causing inhibition of nutrient uptake and productivity. The aim of this study was to evaluate the potential of microbial consortium consisting of arbuscular mycorrhizal fungus (AMF), rhizobia and PGPR for counteracting negative effects of Al toxicity on four pea genotypes differing in Al tolerance. Pea plants were grown in acid soil supplemented with AlCl3 (pHKCl = 4.5) or neutralized with CaCO3 (pHKCl = 6.2). Inoculation increased shoot and/or seed biomass of plants grown in Al-supplemented soil. Nodule number and biomass were about twice on roots of Al-treated genotypes after inoculation. Inoculation decreased concentrations of water-soluble Al in the rhizosphere of all genotypes grown in Al-supplemented soil by about 30%, improved N2 fixation and uptake of fertilizer 15N and nutrients from soil, and increased concentrations of water-soluble nutrients in the rhizosphere. The structure of rhizospheric microbial communities varied to a greater extent depending on the plant genotype, as compared to soil conditions and inoculation. Thus, this study highlights the important role of symbiotic microorganisms and the plant genotype in complex interactions between the components of the soil-microorganism-plant continuum subjected to Al toxicity.

Section: Discussionmentioning

confidence: 99%

“…Portion of roots about 100 mg fresh weight (FW) from each pot was taken to determine root colonization efficiency of Ps. fluorescens SPB2137 using its rifampicin resistance and the root homogenate dilution technique that has been recently described [ 72 ]. The number of Ps.…”

Section: Methodsmentioning

confidence: 99%

The Role of Symbiotic Microorganisms, Nutrient Uptake and Rhizosphere Bacterial Community in Response of Pea (Pisum sativum L.) Genotypes to Elevated Al Concentrations in Soil

Belimov

Шапошников

Syrova

et al. 2020

Self Cite

“…The results of this shift remain to be seen, but the feasibility of such scenarios has already been pledged by the explosive development of advanced genome editing tools [ 393 ]. The next step will likely be the optimization of supraorganism level systems and communities of organisms, the adaptive potential of which has not yet been properly assessed by us; however, it is this potential that can provide not only a further increase in productivity but also a decrease in the need for the use of chemicals [ 394 , 395 ].…”

Section: Discussionmentioning

confidence: 99%

OMICs, Epigenetics, and Genome Editing Techniques for Food and Nutritional Security

Gogolev

Ahmar

Akpınar³

et al. 2021

The incredible success of crop breeding and agricultural innovation in the last century greatly contributed to the Green Revolution, which significantly increased yields and ensures food security, despite the population explosion. However, new challenges such as rapid climate change, deteriorating soil, and the accumulation of pollutants require much faster responses and more effective solutions that cannot be achieved through traditional breeding. Further prospects for increasing the efficiency of agriculture are undoubtedly associated with the inclusion in the breeding strategy of new knowledge obtained using high-throughput technologies and new tools in the future to ensure the design of new plant genomes and predict the desired phenotype. This article provides an overview of the current state of research in these areas, as well as the study of soil and plant microbiomes, and the prospective use of their potential in a new field of microbiome engineering. In terms of genomic and phenomic predictions, we also propose an integrated approach that combines high-density genotyping and high-throughput phenotyping techniques, which can improve the prediction accuracy of quantitative traits in crop species.

“…The effect of PGPR and PGPE on enhanced uptake of heavy metals by Brassica species was reported in most of the recently published studies [99][100][101][102][103][104][105][106], but the efficiency of uptake varied depending on the metal. A positive effect of PGPR inoculation on the uptake and translocation of Cd and Zn and no effect on the uptake of Cu and Pb in B. juncea was observed in an experiment with bacterial strains belonging to the genera Burkholderia containing the enzyme ACC deaminase which controls the production of ethylene in plants and might cause an enhanced uptake of metals through increasing tolerance of stress by reducing ethylene concentration in plants and through modification of root architecture [104].…”

Section: The Use Of Pgprmentioning

confidence: 92%

“…Various studies report increases in plant total biomass from 20 to 60% after PGPR application [106,107]. However, this effect was not observed in some recent studies on PGPR-enhanced phytoextractions with Brassica species [98,100,104,105]. Jinal et al [101] found that inoculation of Brassica juncea seeds with iron-tolerant PGP bacteria enhanced the root length of plants grown in iron contaminated soil from 47.1 to 106.4% and shoot length from 49.40 to 71.71% compared to controls.…”

Section: The Use Of Pgprmentioning

confidence: 98%

Brassica Species in Phytoextractions: Real Potentials and Challenges

Zeremski

Ranđelović

Jakovljević

et al. 2021

The genus Brassica is recognized for including species with phytoaccumulation potential and a large amount of research has been carried out in this area under a variety of conditions, from laboratory experiments to field trials, with spiked or naturally contaminated soils, using one- or multi-element contaminated soil, generating various and sometimes contradictory results with limited practical applications. To date, the actual field potential of Brassica species and the feasibility of a complete phytoextraction process have not been fully evaluated. Therefore, the aim of this study was to summarize the results of the experiments that have been performed with a view to analyzing real potentials and limitations. The reduced biomass and low metal mobility in the soil have been addressed by the development of chemically or biologically assisted phytoremediation technologies, the use of soil amendments, and the application of crop management strategies. Certain issues, such as the fate of harvested biomass or the performance of species in multi-metal-contaminated soils, remain to be solved by future research. Potential improvements to current experimental settings include testing species grown to full maturity, using a greater amount of soil in experiments, conducting more trials under real field conditions, developing improved crop management systems, and optimizing solutions for harvested biomass disposal.