Microbial Contamination Potential and Antibiotic Resistance Profile of Staphylococcus aureus and Escherichia coli Isolated from Ipomoea batatas Leaf

Abstract

Traditional leafy vegetables are recommended because there are important sources of nutrients and they protect against certain non-communicable diseases. This study aimed at assessing the microbiological quality of Ipomoea batatas leaves and evaluating the susceptibility to antibiotics of isolated S. aureus and E. coli strains.
 Heighten samples of I. batatas leaves collected were analyzed for research / enumeration of mesophilic total aerobic flora, enterobacteriaceae, yeasts, molds, fecal coliforms, fecal streptococci, Staphylococcus aureus, Escherichia coli and Salmonella strains and Escherichia coli, Staphylococcus aureus. The microorganisms were identified using appropriate medium and classical biochemical tests. The susceptibility to eleven conventional antibiotics was investigated by the disk diffusion method.
 The I. batatas leaves showed high contamination by various flora, in particular flora indicative of general pollution (aerobic mesophilic germs, yeasts and molds, enterobacteria) and flora of fecal origin (fecal coliforms and fecal streptococcus). In addition, the presence of pathogenic species such as Staphylococcus aureus, Escherichia coli and Salmonella sp. was noted in the samples analyzed. Globally, the highest resistance proportion has been observed with penicillin (~65%) and none of the S. aureus strains was resistant to vancomycin.
 From a microbiological point of view, Ipomoea batatas leaf intended for consumption is not suitable for human consumption. It is therefore important to educate producers to improve their production route in order to have better microbiological quality I. batatas leaves.

Hygienic quality of raw and fermented cow milk in the local milk sector of the Liptako-Gourma area in Niger

“…The mesophilic aerobic germ load was very high in all milk samples from the three regions being over the 10 6 CFU/mL acceptable threshold. The total aerobic mesophilic flora provides information on milk's hygienic quality [31], due to its relationship with non-compliance with good milk production and storage practices [32]. Non-compliance with the standard may render the product unfit for human consumption even in the absence of pathogenic flora [33].…”

ASSESSMENT OF Staphylococcus aureus PREVALENCE IN VEGETABLES SOLD AT LOCAL MARKETS IN BENIN CITY: IMPLICATIONS FOR FOOD SAFETY.

“…In most developing countries, vegetable farmers employ the use of wastewaters for irrigation purposes and animal wastes in soil fertilization to subdue the limited access to clean water and synthetic fertilizers (Kouassi et al, 2019). These practices make fresh vegetables potential vehicles of foodborne microbial pathogens which can be detrimental to the health of consumers (Mir et al, 2018;Attien et al, 2020). Foodborne pathogens could cause disease manifestations resulting from the activities of enterotoxins (Bennett et al, 2013).…”

“…Foodborne pathogens could cause disease manifestations resulting from the activities of enterotoxins (Bennett et al, 2013). Globally, an estimate of about tens of million cases of vegetable-borne diseases often with deaths has been reported, with most cases emerging from Africa (Attien et al, 2020). Several investigations have reported contamination of leafy vegetables by microbes including S. aureus (Seo et al, 2010;Hong et al, 2015).…”

“…Globally, the activities of enterotoxigenic S. aureus are a foremost cause of foodborne diseases of staphylococcal origin (Hennekinne et al, 2012). S. aureus's capability of acquiring determinants of antibiotic resistance to diverse antimicrobial agents has also been reported (Argudin et al, 2012;Jamali et al, 2015;Attien et al, 2020). The alarming surge of antimicrobial-resistance in S. aureus has been detected in several developed and emergent nations over the years (Wang et al, 2017;Kashani et al, 2018).…”