Microbial control of the invasive spiraling whitefly on cassava with entomopathogenic fungi

Boopathi, T.; Karuppuchamy, P.; Singh, Soibam Khogen; Kalyanasundaram, M.; Mohankumar, S.; Ravi, M. V.

doi:10.1590/s1517-838246420141067

Cited by 25 publications

(12 citation statements)

References 13 publications

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“…Lecanicillium species are a group of fungi which may exhibit pathogenic properties both to insects (entomopathogens) and to other fungi and could be isolated from a wide range of substrates. Some species of Lecanicillium have also been used as biocontrol agents .…”

Section: Introductionmentioning

confidence: 99%

Multilocus genotyping based species identification of entomopathogenic fungi of the genus Lecanicillium (=Verticillium lecanii s.l.)

et al. 2017

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Mitochondrial gene NADH dehydrogenase subunit 1 (nad1), β-tubulin gene, and elongation factor 1-alpha (tef) were used to characterize and to identify 42 Lecanicillum spp. isolates (former complex species Verticillium lecanii Zimm. Viegas) and to study the phylogenetic relationships in this group. Within the isolates under investigation, Lecanicillium muscarium was the most common species (about 70% of all isolates, collected on the different hosts, predominantly on the insects from the order Hemiptera). Based on nad1 sequencing four main molecular haplotypes were revealed. All four haplotypes have Holarctic origin. Most of them were isolated in the Central part of Russia. One haplotype showed a specific association with the certain geographical area, limited to southwest Georgia and the Krasnodar Territory. For most strains their affiliation to species L. muscarium, L. longisporum, L. psalliotae, L. pissodes were confirmed by the phylogenetic tree, based on the combined sequences of nad1, β-tub, and tef genes. Only five strains of haplotype C and strain F-2643 could not be identified to any present Lecanicillium species and their position remains ambiguous. Thus, the use of multilocus molecular approach based on these genes was useful to identify the Lecanicillium species. Inter-simple sequence repeat (ISSR) study evaluated a high diversity among the L. muscarium strains. The topology of the NJ-tree based on the ISSR-PCR markers has shown the genetic relationships with the support values 62-91% between L. muscarium isolates.

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Section: Introductionmentioning

confidence: 99%

Multilocus genotyping based species identification of entomopathogenic fungi of the genus Lecanicillium (=Verticillium lecanii s.l.)

et al. 2017

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“…The results of Naglaa (2017) matches what we have found as she tested the same three entomopathogenic fungi against whiteflies on tomato crop and proved V. lecanii gave the greatest effect and highest mortality percentage. Boopathi et al (2015) found similar results when they tested V. lecanii against A. disperses (Hemiptera, Aleyrodidae) and highlighted that V. lecanii exhibited promising levels of control (> 70% mortality) among the other tested fungi which were B. bassiana and M. anisopliae. However, our results were not in accordance with Javed et al (2019) who reported B. bassiana was more effective than V. lecanii against whiteflies under laboratory conditions.…”

Section: Discussionmentioning

confidence: 56%

Comparative Effect of Three Entomopathogenic Fungi against Whitefly Bemisia tabaci (Gennadius) Infesting Eggplant under Field Conditions at Kafr El-Sheik Gov., Egypt

Nada

Hala

Gaffar

et al. 2021

Journal of Plant Protection and Pathology

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Using entomopathogenic fungi, as a powerful bio-control agent, is considered one of the promising methodologies in IPM programs. The current investigation aimed to test the efficiency of Metarhizium anisopliae, Verticillium lecanii, and Beauveria bassiana against whitefly on Eggplant (variety Anann) in Kafr El Sheikh Governorate, Egypt. The experiment was laid in a randomized complete block design, in an area of one feddan, divided into 4 plots (5 replicates each). The study extended from July till September, during two successive seasons (2019 and 2020) and each fungal product was sprayed four times (one spray/week). Temperature fluctuated from 29 to 35 C and RH ranged from 49 to 52%. Results indicated that using entomopathogenic fungi significantly reduced B. tabaci population. V. lecanii was the most influential fungus, as it caused the highest control percentage compared to the other two fungi and control treatment. Repeating application during 4 successive weeks raised fungi effectiveness and caused ascending mortality levels in B. tabaci population. Production rates were in line with percentage reductions of whiteflies. The largest yield of eggplant's fruits was produced in the plots treated with V. lecanii. Accordingly, the study recommends using V. lecanii in whiteflies control strategies in Egypt. Moreover, further investigations are needed to study compatibility of V. lecanii with other bio-agents to avoid inconsistency when applied together in pest management programs.

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“…The present study observed increased mortality of different developmental stages of RSW with an increase in the conidial concentration and time of exposure. Likewise, Boopathi et al [59] also observed that the application of I. fumosorosea was highly pathogenic to A. dispersus on cassava and exhibited differences in efficacy between 3 and 15 days after treatment. In the present study, the LC 50 values and LT 50 values of S. lanosoniveum against eggs, first instar, second to third instar, and pupae of RSW were 4.72 × 10 4 , 4.94 × 10 4 , 5.11 × 10 5 , 5.92 × 10 5 conidia/mL and 4.27, 4.86, 4.56, 5.89 days, respectively.…”

Section: Discussionmentioning

confidence: 94%

Entomopathogenic Potential of Simplicillium lanosoniveum Native Strain in Suppressing Invasive Whitefly, Aleurodicus rugioperculatus Martin (Hemiptera: Aleyrodidae), Infesting Coconut

Sujithra

Prathibha

Rajkumar

et al. 2021

JoF

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In 2016, infestation of an exotic polyphagous pest, the rugose spiraling whitefly (RSW), Aleurodicus rugioperculatus Martin (Hemiptera: Aleyrodidae), was documented on coconut for the first time in India. Instantaneously, RSW has garnered wide attention owing to its damage severity and rapid spread across the coconut-growing regions of the country. Hence, an attempt was made to devise a sustainable integrated pest management (IPM) module using biological control agents as a mainstay component. The present study documented the identification and characterization of a potential entomopathogenic fungal isolate for the management of RSW. An entomopathogenic fungus isolated from nymphal cadavers of RSW was identified as Simplicillium lanosoniveum based on morphological and phylogenetic analyses. A gradient of five conidial concentrations (1 × 104, 1 × 105, 1 × 106, 1 × 107 and 1 × 108 conidia/mL) of the S.lanosoniveum were tested against eggs, first instars, second to third instars and pupae of RSW. Results revealed that S.lanosoniveum is highly virulent to all developmental stages of RSW by causing mortality rates of 95.20%, 87.33%, 85.38% and 72.85%, in eggs, initial, middle and later instar nymphs of RSW, respectively, at the highest tested concentration (1 × 108 conidia/mL) at seven days after exposure. The LC50 and LT50 values of S.lanosoniveum were 4.72 × 104, 4.94 × 104, 5.11 × 105, 5.92 × 105 conidia/mL and 4.27, 4.86, 4.56, 5.89 days against eggs, initial, middle and later instar nymphs of RSW, respectively. Further, preliminary field trials with S.lanosoniveum strain at 1 × 108 conidia/mL exhibited a significant reduction in the egg and nymphal population by 57.8% and 56.3%, respectively. This report thus demonstrated that the newly isolated S.lanosoniveum is an effective pathogen at suppressing all the developmental stages of RSW. This is the first record of S.lanosoniveum infecting RSW, and it has a great potential to be developed as a mycoinsecticide.

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Microbial control of the invasive spiraling whitefly on cassava with entomopathogenic fungi

Cited by 25 publications

References 13 publications

Multilocus genotyping based species identification of entomopathogenic fungi of the genus Lecanicillium (=Verticillium lecanii s.l.)

Multilocus genotyping based species identification of entomopathogenic fungi of the genus Lecanicillium (=Verticillium lecanii s.l.)

Comparative Effect of Three Entomopathogenic Fungi against Whitefly Bemisia tabaci (Gennadius) Infesting Eggplant under Field Conditions at Kafr El-Sheik Gov., Egypt

Entomopathogenic Potential of Simplicillium lanosoniveum Native Strain in Suppressing Invasive Whitefly, Aleurodicus rugioperculatus Martin (Hemiptera: Aleyrodidae), Infesting Coconut

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