Microbial degradation and decolourisation of Reactive Black by an application of Pseudomonas stutzeri ETL-79

Abstract

IntroductionThe textile industry in India is one of the oldest industries. It provides direct employment to nearly thirty million people. Wastewater from the textile industries are a complex mixture of many polluting substances such as organo chlorine-based pesticides, heavy metals, pigments and dyes. The dye that has been chosen for this study is Reactive Black. This study focused on microbial degradation and decolourisation of Reactive Black by an application of Pseudomonas stutzeri ETL-79. Materials and methodsThe decolourising bacterium was identified as P. stutzeri ETL-79 based on physiological and biochemical characteristics as well as the 16S rRNA sequence. In this study, the effects of P. stutzeri ETL-79's decolourising ability, for example, glucose concentration, temperature, pH and dye concentration, were investigated. ResultsThe results show that under 40°C, pH 7.0, glucose concentration 0.2% and concentration of Reactive Black of 30 mg/L the decolourisation rate in 24 and 48 hours was 86.3% and 93.2%, respectively. ConclusionThe study concluded that pH, temperature and various carbon sources have a significant influence on the dye removal efficiency by P. stutzeri ETL-79. This shows that the isolated becomes uneconomical and causes further environmental damage. Hence, economical and eco-friendly techniques using bacteria can be used for fine-tuning of wastewater treatment. Bio-treatment offers an easy, cheap and effective alternative for colour removal of textile dyes.

Isolation of Phenol-Degrading Bacteria from Decapods Mangrove Crabs

“…Microbes found in natural water and soil has broad ability to utilize all naturally and some synthetically occurring compounds. Their sole carbon and energy sources recycling the fixed organic carbon back into harmless biomass and carbon dioxide and resulting in clean up of environment [16]. There were reports on many microorganisms capable of degrading phenol through the action of variety of enzymes [17].…”

Microbial Degradation of Reactive Red by Pseudomonas spp. MPS-2

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