Microbial degradation and detoxification of high molecular weight polycyclic aromatic hydrocarbons by Stenotrophomonas maltophilia strain VUN 10,003

Juhasz, Albert L.; Stanley, Grant A.; Britz, ML

doi:10.1046/j.1472-765x.2000.00733.x

Cited by 146 publications

(82 citation statements)

References 15 publications

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“…Many microorganisms with the capability of degrading different PAHs have been isolated based on culturedependent techniques Juhasz et al 2000;Chang et al 2011). However, it is well known that culture-independent approaches allow for a broader recognition of microbial populations responsible for PAH degradation in environments (Baldwin et al 2003;Jurelevicius et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Response of bacterial pdo1, nah, and C12O genes to aged soil PAH pollution in a coke factory area

Han

Liu

Zheng

et al. 2014

Environ Sci Pollut Res

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Soil pollution caused by polycyclic aromatic hydrocarbons (PAHs) is threatening human health and environmental safety. Investigating the relative prevalence of different PAH-degrading genes in PAH-polluted soils and searching for potential bioindicators reflecting the impact of PAH pollution on microbial communities are useful for microbial monitoring, risk evaluation, and potential bioremediation of soils polluted by PAHs. In this study, three functional genes, pdo1, nah, and C12O, which might be involved in the degradation of PAHs from a coke factory, were investigated by realtime quantitative PCR (qPCR) and clone library approaches. The results showed that the pdo1 and C12O genes were more abundant than the nah gene in the soils. There was a significantly positive relationship between the nah or pdo1 gene abundances and PAH content, while there was no correlation between C12O gene abundance and PAH content. Analyses of clone libraries showed that all the pdo1 sequences were grouped into Mycobacterium, while all the nah sequences were classified into three groups: Pseudomonas, Comamonas, and Polaromonas. These results indicated that the abundances of nah and pdo1 genes were positively influenced by levels of PAHs in soil and could be potential microbial indicators reflecting the impact of soil PAH pollution and that Mycobacteria were one of the most prevalent PAHs degraders in these PAH-polluted soils. Principal component analysis (PCA) and correlation analyses between microbial parameters and environmental factors revealed that total carbon (TC), total nitrogen (TN), and dissolved organic carbon (DOC) had positive effects on the abundances of all PAH-degrading genes. It suggests that increasing TC, TN, and DOC inputs could be a useful way to remediate PAH-polluted soils.

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Section: Introductionmentioning

confidence: 99%

Response of bacterial pdo1, nah, and C12O genes to aged soil PAH pollution in a coke factory area

Han

Liu

Zheng

et al. 2014

Environ Sci Pollut Res

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“…strain CHY-1 (26)(27)(28) were also documented. Biodegradation of benz [a]anthracene without documentation of metabolites has been reported to have occurred by members of the genera Alcaligenes (29), Stenotrophomonas (30)(31)(32), Sphingomonas (33), and Pseudomonas (34,35).…”

mentioning

confidence: 99%

Benz[ a ]anthracene Biotransformation and Production of Ring Fission Products by Sphingobium sp. Strain KK22

Kunihiro

Ozeki

Nogi

et al. 2013

Appl Environ Microbiol

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A soil bacterium, designated strain KK22, was isolated from a phenanthrene enrichment culture of a bacterial consortium that grew on diesel fuel, and it was found to biotransform the persistent environmental pollutant and high-molecular-weight polycyclic aromatic hydrocarbon (PAH) benz[a]anthracene. Nearly complete sequencing of the 16S rRNA gene of strain KK22 and phylogenetic analysis revealed that this organism is a new member of the genus Sphingobium. An 8-day time course study that consisted of whole-culture extractions followed by high-performance liquid chromatography (HPLC) analyses with fluorescence detection showed that 80 to 90% biodegradation of 2.5 mg liter H igh-molecular-weight polycyclic aromatic hydrocarbons (HMW PAHs) are commonly occurring environmental pollutants that are generally considered to be more resistant to biodegradation than their lower-molecular-weight aromatic counterparts (1-4). Many are suspected carcinogens and display genotoxic and immunotoxic properties in addition to causing oxidative cell damage (5, 6). The HMW PAH benz [a]anthracene is considered to be environmentally recalcitrant, is classified as a group 2A carcinogen by the International Agency for Research on Cancer, and is included in the U.S. Environmental Protection Agency's Priority Pollutant List. As such, there is much interest in understanding the environmental fate of benz[a]anthracene and the mechanisms by which it may be transformed.Few studies have documented the bacterial biotransformation of benz [a]anthracene even though many studies have documented the biotransformation of the structurally similar threering angular kata-annelated PAH phenanthrene (7-16) and, although less so, also the structurally similar three-ring linear kata-annelated PAH anthracene (7,13,(17)(18)(19). The benz [a]anthracene molecule itself is comprised of four aromatic rings that are bonded via both linear and angular kata annelation, and it may be thought of as a benzannelated derivative of either phenanthrene or anthracene. Initial enzymatic oxidation of the aromatic ring system of benz [a]anthracene may occur at various locations on the molecule, including via the 1,2-or 3,4-carbon positions, an angular kata-type initial dioxygenation, via the 8,9-or 10,11-carbon positions, a linear kata-type initial dioxygenation, or via the K-region at the 5,6-carbon positions. If metabolites that represent the initial oxidation steps are not directly recovered in metabolism studies, identification of downstream metabolites may allow for predicting whether an angular kata-, linear kata-, or K region-type initial dioxygenation originally occurred. For example, 2-hydroxy-3-naphthoic acid may occur as a downstream metabolite of benz [a]anthracene biotransformation through an angular katatype initial dioxygenation event.To date, metabolites from the biotransformation of benz[a]anthracene by bacteria have been identified from only four organisms. In chronological order, they are (i) Sphingobium yanoikuyae mutant strain B8/36 (20-22), (ii) S. yanoiku...

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“…PAH degradation by Pseudomonas species is well reported. [50][51][52][53][54] Degradation of PAHs using Stenotrophomonas 55 , in particular Stenotrophomonas maltophilia [56][57][58] , has been investigated. Hydrocarbon degradation capabilities for some of these species have also been demonstrated with aliphatic 59 and aromatic hydrocarbons 60 .…”

Section: Degradation Of Nonylphenol By Bacterial Speciesmentioning

confidence: 99%

Isolation and characterisation of endocrine disruptor nonylphenol-using bacteria from South Africa

Qhanya¹,

Mthakathi²,

Boucher³

et al. 2017

S. Afr. J. Sci.

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Endocrine disrupting chemicals (EDCs) are synthetic chemicals that alter the function of endocrine systems in animals including humans. EDCs are considered priority pollutants and worldwide research is ongoing to develop bioremediation strategies to remove EDCs from the environment. An understanding of indigenous microorganisms is important to design efficient bioremediation strategies. However, much of the information available on EDCs has been generated from developed regions. Recent studies have revealed the presence of different EDCs in South African natural resources, but, to date, studies analysing the capabilities of microorganisms to utilise/degrade EDCs have not been reported from South Africa. Here, we report for the first time on the isolation and enrichment of six bacterial strains from six different soil samples collected from the Mpumalanga Province, which are capable of utilising EDC nonylphenol as a carbon source. Furthermore, we performed a preliminary characterisation of isolates concerning their phylogenetic identification and capabilities to degrade nonylphenol. Phylogenetic analysis using 16S rRNA gene sequencing revealed that four isolates belonged to Pseudomonas and the remaining two belonged to Enterobacteria and Stenotrophomonas. All six bacterial species showed degradation of nonylphenol in broth cultures, as HPLC analysis revealed 41–46% degradation of nonylphenol 12 h after addition. The results of this study represent the beginning of identification of microorganisms capable of degrading nonylphenol, and pave the way for further exploration of EDC-degrading microorganisms from South Africa.

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Microbial degradation and detoxification of high molecular weight polycyclic aromatic hydrocarbons by Stenotrophomonas maltophilia strain VUN 10,003

Cited by 146 publications

References 15 publications

Response of bacterial pdo1, nah, and C12O genes to aged soil PAH pollution in a coke factory area

Response of bacterial pdo1, nah, and C12O genes to aged soil PAH pollution in a coke factory area

Benz[ a ]anthracene Biotransformation and Production of Ring Fission Products by Sphingobium sp. Strain KK22

Isolation and characterisation of endocrine disruptor nonylphenol-using bacteria from South Africa

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