Microbial Degradation and Preliminary Chemical Characterization of <i>Microcystis</i> Exopolysaccharides from a Cyanobacterial Water Bloom of Lake Taihu

Li, Pengfu; Cai, Yuanfeng; Shi, Limei; Lanfang, GENG; Xing, Peng; Yang, Yu; Kong, Fanxiang; Wang, Yujiong

doi:10.1002/iroh.200911149

Cited by 23 publications

(9 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was previously observed that polysaccharides were secreted during frequent and extensive bloom events in lakes dominated by colony‐forming Microcystis (Li et al. ). A recent study reported that colonial Microcystis could secret more soluble polysaccharides into the medium than the unicellular form (Zhang et al.…”

Section: Discussionmentioning

confidence: 97%

“…We have shown in this study that colonial Microcystis possesses the advantage of physiological acclimation over the unicellular form when growing under iron-deficient conditions. It was previously observed that polysaccharides were secreted during frequent and extensive bloom events in lakes dominated by colony-forming Microcystis (Li et al 2009). A recent study reported that colonial Microcystis could secret more soluble polysaccharides into the medium than the unicellular form ).…”

mentioning

confidence: 97%

See 1 more Smart Citation

Capsular polysaccharides facilitate enhanced iron acquisition by the colonial cyanobacterium Microcystis sp. isolated from a freshwater lake

Dai

Juneau

et al. 2016

Journal of Phycology

View full text Add to dashboard Cite

Microcystis sp., especially in its colonial form, is a common dominant species during cyanobacterial blooms in many iron-deficient water bodies. It is still not entirely clear, however, how the colonial forms of Microcystis acclimate to iron-deficient habitats, and the responses of unicellular and colonial forms to iron-replete and iron-deficient conditions were examined here. Growth rates and levels of photosynthetic pigments declined to a greater extent in cultures of unicellular Microcystis than in cultures of the colonial form in response to decreasing iron concentrations, resulting in the impaired photosynthetic performance of unicellular Microcystis as compared to colonial forms as measured by variable fluorescence and photosynthetic oxygen evolution. These results indicate that the light-harvesting ability and photosynthetic capacity of colonial Microcystis was less affected by iron deficiency than the unicellular form. The carotenoid contents and nonphotochemical quenching of colonial Microcystis were less reduced than those of the unicellular form under decreasing iron concentrations, indicating that the colonial morphology enhanced photoprotection and acclimation to iron-deficient conditions. Furthermore, large amounts of iron were detected in the capsular polysaccharides (CPS) of the colonies, and more iron was found to be attached to the colonial Microcystis CPS under decreasing iron conditions as compared to unicellular cultures. These results demonstrated that colonial Microcystis can acclimate to iron deficiencies better than the unicellular form, and that CPS plays an important role in their acclimation advantage in iron-deficient waters.

show abstract

Section: Discussionmentioning

confidence: 97%

mentioning

confidence: 97%

Capsular polysaccharides facilitate enhanced iron acquisition by the colonial cyanobacterium Microcystis sp. isolated from a freshwater lake

Dai

Juneau

et al. 2016

Journal of Phycology

View full text Add to dashboard Cite

show abstract

“…On the contrary, the presence of deoxy sugars rhamnose and fucose in side chains, which are known for their hydrophobic properties due to the methyl group at carbon 6, increases the hydrophobicity and stickness of the extracellular polysaccharide of A. granulate (VIEIRA et al, 2008). The predominance of rhamnose may increase the hydrophobic feature of the extracellular polysaccharide of Microcystis (LI et al, 2009). Further studies on chemical structure of extracellular polysaccharide in Microcystis are urgent to be performed in order to elucidate the function of extracellular polysaccharide in cell hydrophobicity.…”

Section: Discussionmentioning

confidence: 99%

Role of Cell Hydrophobicity on Colony Formation in Microcystis (Cyanobacteria)

Yang

Cai

Xia

et al. 2011

International Review of Hydrobiology

Self Cite

View full text Add to dashboard Cite

Colony formation is highly import ant for the competitive advantage of the cyanobacterium Microcystis over other phytoplankton species. The laboratory-grown colonial Microcystis strains isolated from Lake Taihu (China) maintained colonial forms under the low light condition (10 μE m ) leaded to the significant decrease in the cell hydrophobicities of the colonial Microcystis and the transition from colonial forms to unicellular forms. These findings indicated that the cell hydrophobicity of Microcystis may play a role in cell-cell adherence and colony formation. Phosphate-limitation, nitrate-limitation and pH did not affect cell hydrophobicities of colonial Microcystis. Treatment with proteolytic enzymes had no effect on the cell hydrophobicity, indicating that cell surface proteins did not contribute to high cell hydrophobicity. IntroductionAs a result of anthropogenic eutrophication, environmental problems caused by cyanobacterial blooms in lakes, ponds, rivers and reservoirs have been increasingly documented across the world (LEHMAN, 2005;KEMP and JOHN, 2006). Microcystis spp. are common bloomforming cyanobacteria. It is already known that, under natural conditions, Microcystis occurs as colonial aggregates constrained by an amorphous mucilage or sheath (OTSUKA et al., 2000). However, during long-term storage and cultivation under laboratory conditions, the characteristics of colonies disappear, and Microcystis exists mainly as single cells and a few paired cells (ZHANG et al., 2007). Colony formation is highly important for the competitive advantage of Microcystis over other phytoplankton species. It is suggested that formation of Microcystis colonies is helpful to vertical migration and defense against predation pressure 142 H. YANG et al.

show abstract

“…In the case of Microcystis, the position relative to the surface can be achieved thanks to the presence of gas vesicles aggregations or aerotopes in the cytoplasm (Šmarda and Maršálek, 2008), which allow them to regulate their vertical position in the water column and to form the colony in a suitable position receiving the right amount of light, oxygen and CO2. The EPS matrix of bacteria that are able to form pellicles are usually composed by glucose, galactose, rhamnose, mannose or cellulose (for a review see Armitano et al 2014), which are also typical components of the extracellular matrix of Microcystis (Lei et al, 2007;Li et al, 2009). Thus, Microcystis colonies have more than one trait contributing to float near the surface.…”

Section: Main Characteristics Of Bacterial Biofilmsmentioning

confidence: 99%

New insight into colonies of Microcystis (Cyanobacteria) as multi-specific floating biofilms

Piccini¹,

Segura²,

Escalera³

et al. 2022

Preprint

View full text Add to dashboard Cite

The ability to form biofilms is a functional trait shared by many bacterial species. Biofilms provide bacteria a sheltered environment where the nutrients and oxygen gradients create a heterogeneous matrix and promote cells to differentiate their metabolism and functions according to the position they occupy inside the matrix. Species of the Microcystis genus are among the most common bloom-forming cyanobacteria. They are unicellular microorganisms able to form colonies and to reach high biomass during blooms in lakes, reservoirs and estuaries worldwide. Colonial lifestyle provides several advantages under stressing conditions, including adaptation to different light intensities, protection from toxic substances and grazing, while allowing them to grow when the nutrient supply is low. Although the biology, ecology and colony formation have been extensively recognized in Microcystis spp., the analysis of the progression from unicellular to multicellular phases in this cyanobacterium have been always addressed as individual phenotypic plasticity and rarely as a multi-specific community of interrelated microorganisms. Here, we re-interpreted the evidence coming from different studies about the Microcystis lifestyle and propose a new way to analyze the available information about this cyanobacterial group. We specifically address the characteristics shared by bacterial biofilms and Microcystis colonies and suggest that the morphological changes from single cells to colonies are due to a cascade of events leading to the formation of a multi-specific biofilm. Studying the formation of colonies using this framework would help to better understand the life cycle of Microcystis, its functional relationship with the associated microbiome and the factors triggering microcystin production, helping to design strategies for prevention and control of the blooms caused by these organisms. Taking into account the biology and the ecological strategies of Microcystis, a conceptual model of emergence and decay of these floating multi-specific biofilms is proposed.

show abstract

Microbial Degradation and Preliminary Chemical Characterization of Microcystis Exopolysaccharides from a Cyanobacterial Water Bloom of Lake Taihu

Cited by 23 publications

References 36 publications

Capsular polysaccharides facilitate enhanced iron acquisition by the colonial cyanobacterium Microcystis sp. isolated from a freshwater lake

Capsular polysaccharides facilitate enhanced iron acquisition by the colonial cyanobacterium Microcystis sp. isolated from a freshwater lake

Role of Cell Hydrophobicity on Colony Formation in Microcystis (Cyanobacteria)

New insight into colonies of Microcystis (Cyanobacteria) as multi-specific floating biofilms

Contact Info

Product

Resources

About