Microbial degradation kinetics of solid alkane dissolved in nondegradable oil phase

Choi, Dong Hyuk; Hori, Katsutoshi; Tanji, Yasunori; Unno, Hajime

doi:10.1016/s1369-703x(99)00004-2

Cited by 15 publications

(10 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For alkanes, μ max and K s values of 0.6 h -1 and 86.0 mg/L were used based on literature values for heneicosane [17]. For BTEX, a μ max value of 0.32 h -1 and a K s value of 129.2 mg/L was used based on averaged values from literature data [16,43-47].…”

Section: Resultsmentioning

confidence: 99%

“…The majority of existing models assume that spilled oil is in soluble form [14-16]. Few biodegradation models have also been proposed to take into account the presence of oil droplets [17-19]. Among these, oil droplets have been assumed of uniform size with the presence of abundant oil degrading microbes, which is not applicable for most cases.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A new model for the biodegradation kinetics of oil droplets: application to the Deepwater Horizon oil spill in the Gulf of Mexico

Vilcáez

Hubbard

2013

Geochem Trans

View full text Add to dashboard Cite

Oil biodegradation by native bacteria is one of the most important natural processes that can attenuate the environmental impacts of marine oil spills. Existing models for oil biodegradation kinetics are mostly for dissolved oil. This work developed a new mathematical model for the biodegradation of oil droplets and applied the model to estimate the time scale for oil biodegradation under conditions relevant to the Deepwater Horizon oil spill in the Gulf of Mexico. In the model, oil is composed of droplets of various sizes following the gamma function distribution. Each oil droplet shrinks during the microbe-mediated degradation at the oil-water interface. Using our developed model, we find that the degradation of oil droplets typically goes through two stages. The first stage is characterized by microbial activity unlimited by oil-water interface with higher biodegradation rates than that of the dissolved oil. The second stage is governed by the availability of the oil-water interface, which results in much slower rates than that of soluble oil. As a result, compared to that of the dissolved oil, the degradation of oil droplets typically starts faster and then quickly slows down, ultimately reaching a smaller percentage of degraded oil in longer time. The availability of the water-oil interface plays a key role in determining the rates and extent of degradation. We find that several parameters control biodegradation rates, including size distribution of oil droplets, initial microbial concentrations, initial oil concentration and composition. Under conditions relevant to the Deepwater Horizon spill, we find that the size distribution of oil droplets (mean and coefficient of variance) is the most important parameter because it determines the availability of the oil-water interface. Smaller oil droplets with larger variance leads to faster and larger extent of degradation. The developed model will be useful for evaluating transport and fate of spilled oil, different remediation strategies, and risk assessment.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A new model for the biodegradation kinetics of oil droplets: application to the Deepwater Horizon oil spill in the Gulf of Mexico

Vilcáez

Hubbard

2013

Geochem Trans

View full text Add to dashboard Cite

show abstract

“…Arab Light was introduced to an initial crude oil concentration in the reactors of 1% v/v (8.6 g/L, density 0.86 g/mL). This 1% oil/water ratio is the maximum recommended value for a stable dispersion of oil in water without coalescence of the oil drops [39]. A control experiment (without bacteria inoculum in the sterilized bioreactor) was also performed.…”

Section: Bioreactorsmentioning

confidence: 99%

Mechanism of Crude Oil Biodegradation in Bioreactors: A Model Approach

Costa,

Millán

2024

Water

View full text Add to dashboard Cite

Oil-degrading bacteria have the ability to degrade alkanes present in crude oil because of a special enzymatic system, the alkane hydroxylase complex (AlkH). The mechanism for the transport and degradation of alkanes present in crude oil remains unclear, especially related to the first step in hydrocarbons oxidation. In this work, we present a novel model of the crude oil biodegradation mechanism by considering the contact between the oil drop and the cell and calculating the mass transfer coefficients in three oleophilic bacteria (B. licheniformis, P. putida and P. glucanolyticus). The mass transfer coefficients are evaluated under critical time conditions, when the kinetics and mass transport are in balance, and the difference in the values obtained (kL α = 1.60 × 10−3, 5.25 × 10−4 and 6.19 × 10−4 m/d, respectively) shows the higher value of the mass transfer coefficient and higher biodegradation potential for B. licheniformis. Because the morphology of the cells has been analyzed by optical and electron microscopy, in the proposed model, the increase in the size of the cells in P. glucanolyticus compared to P. putida exhibits higher values of the mass transfer coefficients and this is attributed, as a novel statement, to a bigger window for alkanes transport (contact area) when the external area of the cell is bigger.

show abstract

“…2a. PHCs can decrease the hydrolytic acidification rate and reduce system stability (Choi et al 1999;Hu et al 2013). A high proportion of heneicosane (6.49% and 0.94 g/kg-TS) and 1-phenylnaphthalene (6.39% and 0.75 g/kg-TS) were detected and are typically abundant in PREAS.…”

Section: Characterization Of Preasmentioning

confidence: 99%

“…However, due to their high concentrations both heneicosane and 1-phenylnaphthalene remained after fermentation. While heneicosane and 1-phenylnaphthalene are considered to be refractory organic pollutants having strong toxicity (Choi et al 1999), hydrolytic acidification was not inhibited. These results show that thermophilic fermentation is resistant to these highly toxic compounds and can to degrade high molecular weight contaminants, reducing the toxicity of PREAS.…”

Section: Oil Conversion In the Hydrolysis And Acidification Systemmentioning

confidence: 99%

Hydrolysis and acidification of activated sludge from a petroleum refinery

et al. 2019

View full text Add to dashboard Cite

The cost-effective treatment of activated sludge that is generated by refining petroleum is a challenging industrial problem. In this study, semi-continuous stirred tank reactors (CSTRs) containing petroleum refinery excess activated sludge (PREAS) were used to comparatively investigate hydrolysis and acidification rates, after the addition of heneicosane (C 21 H 44 ) (R1) and 1-phenylnaphthalene (C 16 H 12 ) (R2) to different and individual reactors. Operation of the reactors using a sludge retention time (SRT) of 6 days and a pH of 5.0, resulted in the maintenance of stable biological activity as determined by soluble chemical oxygen demand (SCOD), volatile fatty acids (VFAs) production and oil removal efficiency. The optimum conditions for hydrogen production include a SRT of 8 days, at pH 6.5. Under these conditions, hydrogen production rates in the control containing only PREAS were 1567 mL/L (R0), compared with 1365 mL/L in R1 and 1454 mL/L-PREAS in R2. Coprothermobacter, Fervidobacterium, Caldisericum and Tepidiphilus were the dominant bacterial genera that have the potential to degrade petroleum compounds and generate VFAs. This study has shown that high concentrations of heneicosane and 1-phenylnaphthalene did not inhibit the hydrolytic acidification of PREAS.

show abstract

Microbial degradation kinetics of solid alkane dissolved in nondegradable oil phase

Cited by 15 publications

References 12 publications

A new model for the biodegradation kinetics of oil droplets: application to the Deepwater Horizon oil spill in the Gulf of Mexico

A new model for the biodegradation kinetics of oil droplets: application to the Deepwater Horizon oil spill in the Gulf of Mexico

Mechanism of Crude Oil Biodegradation in Bioreactors: A Model Approach

Hydrolysis and acidification of activated sludge from a petroleum refinery

Contact Info

Product

Resources

About