Microbial Degradation of Azo Dye by &lt;i&gt;Pseudomonas spp&lt;/i&gt;. MPS-2 by an Application of Sequential Microaerophilic &amp;amp; Aerobic Process

Shah, Maulin P.; Patel, Kavita A; Nair, Sunu S; Darji, A M; Maharaul, Shaktisinh J

doi:10.12691/ajmr-1-4-7

Cited by 20 publications

(10 citation statements)

References 61 publications

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“…No significant activity has been observed at 60°C for strain ASU6. These came in context with many literatures which study the optimum condition for azoreductase [46,47].…”

Section: Tablementioning

confidence: 89%

“…Many bacteria possess a specific cytoplasmic azoreductases system that catalyzed the electron transfer from NADH (electron donor) to azo bonds. So, the presence of NADH is very significant in the reduction of azodyes by aerobic bacteria [46]. Thus, it could be concluded that, the aerobic azoreductase enzyme is an inducible enzyme required redox mediator for perfect performance.…”

Section: Determination Azoreductase Specific Activity and Substrate Smentioning

confidence: 99%

“…Azoreductase is a key enzyme catalyzes the reduction of azodyes in versatile microorganisms. This enzyme could be inhibited by oxygen [46]. The specific activity of azoreductase enzyme (U/ mg protein) was monitored for the two strains during the growth of strains on methyl red (MR), DB64 or AY17 as specific substrates in the presence of NADH as the electron donor (oxidative mediators).…”

Section: Determination Azoreductase Specific Activity and Substrate Smentioning

confidence: 99%

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Azoreductase kinetics and gene expression in the synthetic dyes-degrading Pseudomonas

Elfarash

Mawad

Yousef

et al. 2017

Egyptian Journal of Basic and Applied Sciences

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Microorganisms have an effective capability to degrade synthetic dyestuff enzymatically. In this study, two 16S rRNA identified Pseudomonas aeruginosa bacterial isolates (ASU3 and ASU6) have been screened to enzymatically degrade both Disperse Blue 64 (DB64) and Acid Yellow 17 (AY17) which resembles 2 of the synthetic dyes that causes aquatic environmental pollution. The expression of the azoR1 gene has been detected in the strains, the degradation percentage of DB64 and AY17 by stain ASU6 was found to exceed the ASU3. This returned to the differences in the expression levels of azoR1 between the ASU6 and the ASU3 which was confirmed by RT-PCR. In addition, the specific activity (U/mg protein) of azoreductase in ASU6 was higher than ASU3 when MethylRed (MR), DB64 and AY17 were used as a substrate, separately. The azoreductase kinetics was studied to find the optimum condition for maintaining the stability of the enzyme. The degraded products separated by HPLC/MS were tested for their toxicity on two crop plants and they were nontoxic to the plants.

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“…No significant activity has been observed at 60°C for strain ASU6. These came in context with many literatures which study the optimum condition for azoreductase [46,47].…”

Section: Tablementioning

confidence: 89%

Section: Determination Azoreductase Specific Activity and Substrate Smentioning

confidence: 99%

Section: Determination Azoreductase Specific Activity and Substrate Smentioning

confidence: 99%

See 1 more Smart Citation

Azoreductase kinetics and gene expression in the synthetic dyes-degrading Pseudomonas

Elfarash

Mawad

Yousef

et al. 2017

Egyptian Journal of Basic and Applied Sciences

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“…possuem a enzima azorredutase (LUCAS et al, 2006;DEIVASIGAMANI;DAS, 2011), como observado nas Figuras 4, 5, 6, 7, 8 e 9, nos ensaios fluorogênicos realizados neste estudo não foi verificada biotransformação do substrato pelas cepas investigadas. Esse resultado pode ser explicado pela necessidade de ambiente microaerofílico para a atividade da enzima azorredutase, sendo que em micro-organismos aeróbicos, seu desempenho ideal é condicionado a um mediador redox (SHAH et al, 2013;ELFARASH et al, 2017). Deve ser levado em consideração que a enzima atua em pH e temperatura ideais e, a variável pH não foi controlada neste estudo.…”

Section: Figura 4: Espectro Ftir/atr Do Composto Hfazbo--no2unclassified

Síntese e avaliação de azoderivado de 2-fenilbenzoxazol como substrato fluorogênico na diferenciação de Candida spp.

Souza

Corbellini

2019

RJP

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Neste estudo foram sintetizados três derivados de 2-fenilbenzoxazol (2FBO) funcionalizados com a função azo: (2’-hidroxi-5’-fenilazofenil)benzoxazol (HFAZBO), 2-(2’-hidroxi-5’-(4’’-carboxi)fenilazofenil)benzoxazol (HFAZBO-CO2H) e 2-(2’-hidroxi-5’-(4’’-nitro)fenilazofenil)benzoxazol (HFAZBO-NO2).Os compostos foram utilizados como substratos fluorogênicos para agentes de infecção urinária, representantes de Candida spp., para explorar suas características metabólicas. A caracterização estrutural dos compostos foi realizada por meio de Espectroscopia de Absorção Molecular no Infravermelho com transformada de Fourier (FT-IR) e por determinação da faixa de fusão. Os ensaios fluorogênicos foram realizados em espécies de Candida spp.: Candida albicans ATCC 10231, Candida famata RL38, Candida glabrata 06130L, Candida glabrata RL43, Candida glabrata 0030L, Candida guilliermondii TH07, Candida krusei ATCC 6258, Candida krusei 0037L, Candida parapsilosis (RL33), Candida parapsilosis (RL38), Candida stellatoidea (9875), Candida stellatoidea (3613), Candida tropicalis ATCC 750. Não foi observada alteração sob luz uv nas leveduras testadas. Do mesmo modo, o extrato obtido a partir das colônias não apresentou fluorescência sob luz uv. Dessa forma, faz-se necessário desenvolver novos estudos que avaliem a função metabólica de Candida spp. a partir da biotransformação levando a liberação de moléculas fluorescentes.

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“…A range of conventional biological, physico-chemical and chemical treatment technologies for dye removal including microbial degradation [5][6][7][8], adsorbent (e.g. active carbon, natural cellulose-based fibre) [9,10], sacrificial iron electrodes [11], electrolysis [12][13][14], electrocoagulation [15,16], ion-pair extraction [17], Fenton's process and advanced electrochemical oxidation process [18][19][20] has hitherto been reported.…”

mentioning

confidence: 99%

Kinetic model and thermodynamic study of Acid Red 1 entrapment at electropolymerised polypyrrole films

Haque

Wong

2015

Journal of Colloid and Interface Science

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Microbial Degradation of Azo Dye by <i>Pseudomonas spp</i>. MPS-2 by an Application of Sequential Microaerophilic & Aerobic Process

Cited by 20 publications

References 61 publications

Azoreductase kinetics and gene expression in the synthetic dyes-degrading Pseudomonas

Azoreductase kinetics and gene expression in the synthetic dyes-degrading Pseudomonas

Síntese e avaliação de azoderivado de 2-fenilbenzoxazol como substrato fluorogênico na diferenciação de Candida spp.

Kinetic model and thermodynamic study of Acid Red 1 entrapment at electropolymerised polypyrrole films

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