Microbial degradation of beta-chlorinated four-carbon aliphatic acids

Abstract

Alcaligenes sp. strain CC1 is able to grow on several a-chlorinated aliphatic acids (2-chlorobutyrate, 2-chloropropionate, and chloroacetate), as wel as on the n-chlorinated four-carbon aliphatic acids trans-3-chlorocrotonate, cis-3-chlorocrotonate, and 3-chlorobutyrate as sole carbon and energy sources. Dehalogenation of a-chlorinated acids could be measured by using resting cells grown on all the different carbon sources, whereas dehalogenation of P-chlorinated four-carbon acids could be detected only by using resting ceUls grown on four-carbon compounds. A constitutive 2-haloacid dehalogenase, which did not show any activity with ,8-chlorinated four-arbon acids, was detected in cell extracts. Cell extracts of crotonate-grown cells additionally contained a 1-haloacid dechlorination activity, which acted on trans-3-chlorocrotonate, cis-3-chlorocrotonate, and 3-chlorobutyrate and was strictly dependent on coenzyme A, ATP, and Mge.Dechlorination of 13-chlorinated four-carbon acids takes place after activation of the acids to their coenzyme A derivatives and seems to be independent of the constitutive 2-haloacid dehalogenase.

Degradation of 3-chloro-2-methylpropionic acid by Xanthobacter sp. CIMW 99

“…CMPA is further catabolized by a pathway similar to that proposed for other chlorinated aliphatic acids. Kohler-Staub and Kohler (1989) demonstrated that the dechlorination (and hence degradation) of trans-3-chlo- rocrotonic acid and 3-chlorobutyric acid required that both the molecules underwent prior conversion to the corresponding CoA derivatives. We found the dehalogenation of CMPA to be CoA-dependent and the reaction involved appeared not to be stereospecific.…”

Degradation of halogenated aliphatic compounds: The role of adaptation

“…[27] Pseudomonas putida [28] 'Coryneform' [29] Pseudomonas cepacia [30] Ah'aligenes sp. CC1 [31 ] pounds that do support growth are expected to have evolved such that their activity with these synthetic molecules is increased. Biochemical recalcitrance thus is a problem of enzyme specificity and activity.…”

Degradation of halogenated aliphatic compounds: The role of adaptation

“…CC1 [31 ] pounds that do support growth are expected to have evolved such that their activity with these synthetic molecules is increased. Biochemical recalcitrance thus is a problem of enzyme specificity and activity.…”

“…Kohler-Staub and Kohler [31] have characterized an Alcaligenes sp., strain CC1, that grows with 3-chlorocrotonic and 3-chlorobutyric acid as sole carbon and energy source. Dechlorination of these fourcarbon acids occurs after activation of the acids …”