Microbial degradation of low density polyethylene

Abraham, Jayanthi; Ghosh, Enoch; Mukherjee, Prantik; Gajendiran, Anudurga

doi:10.1002/ep.12467

Cited by 114 publications

(33 citation statements)

References 42 publications

(50 reference statements)

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“…Saccharothrix wayandensis, etc.) have shown either weight reduction or partial degradation of PE films (Sivan et al, 2006;Abraham et al, 2017;Huang et al, 2019;Soulenthone et al, 2020). Among the fungal species, members of Aspergillus (Muhonja et al, 2018); Fusarium (Sivan, 2011); Penicillium, Zalerion (Raddadi and Fava, 2019); Chaetomium and Pullularia (Sudhakar et al, 2008) are well known for biodegradation of LDPEs and HDPEs.…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of Unpretreated Low-Density Polyethylene (LDPE) by Stenotrophomonas sp. and Achromobacter sp., Isolated From Waste Dumpsite and Drilling Fluid

et al. 2020

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Exploring the catabolic repertoire of natural bacteria for biodegradation of plastics is one of the priority areas of biotechnology research. Low Density Polyethylene (LDPE) is recalcitrant and poses serious threats to our environment. The present study explored the LDPE biodegradation potential of aerobic bacteria enriched from municipal waste dumpsite and bentonite based drilling fluids from a deep subsurface drilling operation. Considerable bacterial growth coupled with significant weight loss of the LDPE beads (∼8%), change in pH to acidic condition and biofilm cell growth around the beads (CFU count 105–106/cm2) were noted for two samples (P and DF2). The enriched microbial consortia thus obtained displayed high (65–90%) cell surface hydrophobicity, confirming their potential toward LDPE adhesion as well as biofilm formation. Two LDPE degrading bacterial strains affiliated to Stenotrophomonas sp. and Achromobacter sp. were isolated as pure culture from P and DF2 enrichments. 16S rRNA gene sequences of these isolates indicated their taxonomic novelty. Further biodegradation studies provided strong evidence toward the LDPE metabolizing ability of these two organisms. Atomic Fore Microscopy (AFM) and Scanning Electron Microscopy (SEM) revealed considerable damage (in terms of formation of cracks, grooves, etc.) on the micrometric surface of the LDPE film. Analysis of the average roughness (Ra), root mean square roughness (Rq), average height (Rz), maximum peak height (Rp), and maximum valley depth (Rv) (nano-roughness parameters) through AFM indicated 2–3 fold increase in nano-roughness of the LDPE film. FTIR analysis suggested incorporation of alkoxy (1000–1090 cm–1), acyl (1220 cm–1), nitro (1500–1600 cm–1), carbonyl (1720 cm–1) groups into the carbon backbone, formation of N-O stretching (1360 cm–1) and chain scission (905 cm–1) in the microbially treated LDPEs. Increase in carbonyl index (15–20 fold), double bond index (1.5–2 fold) and terminal double bond index (30–40 fold) confirmed that biodegraded LDPEs had undergone oxidation, vinylene formation and chain scission. The data suggested that oxidation and dehydrogenation could be the key steps allowing formation of low molecular weight products suitable for their further mineralization by the test bacteria. The study highlighted LDPE degrading ability of natural bacteria and provided the opportunity for their development in plastic remediation process.

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Section: Introductionmentioning

confidence: 99%

Biodegradation of Unpretreated Low-Density Polyethylene (LDPE) by Stenotrophomonas sp. and Achromobacter sp., Isolated From Waste Dumpsite and Drilling Fluid

et al. 2020

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“…The current trend is the utilization of several potential microbes especially bacteria for decontaminating the cultivated lands from chemically synthesized pesticides. Several studies have reported the elimination of such toxic chemicals using microbial degradation as well as synthetic polymers in environment‐friendly ways [20–22]. Considering the alarming consequence of carbofuran toxicity, an LC 50 of carbofuran (99%) was measured against A. salina , which was 9.103558 μg/ml.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of soil‐borne Enterobacter sp. for carbofuran degradation: HPLC quantitation of degradation rate

Ekram

Sarker

Rahi³

et al. 2020

J Basic Microbiol

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Excessive use of pesticides in agricultural fields is a matter of great concern for living beings as well as the environment across the world, in particular, the third world countries. Therefore, there is an urgent need to find out an effective way to degrade these hazardous chemicals from the soil in an environment-friendly way. In the current project, a bacterial species were isolated through enrichment culture from carbofuran-supplemented rice-field soil and identified as a carbofuran degrader. The rate of carbofuran degradation by this bacterial species was evaluated using reverse-phase high-performance liquid chromatography (RP-HPLC), which confirmed the ability to utilize as a carbon source up to 4 µg/ml of 99% technical grade carbofuran. The morphological, physiological, biochemical characteristics and phylogenetic analysis of the 16S rRNA sequence showed that this strain belongs to the genus of Enterobacter sp. (sequence accession number LC368285 in DDBJ), and the optimum growth condition for the isolated strain was 37°C at pH 7.0. Moreover, an antibiotic sensitivity test showed that it was susceptible to azithromycin, penicillin, ceftazidime, ciprofloxacin, and gentamycin, and the minimal inhibitory concentration value of gentamycin was 400 μg/ml against the bacteria. It shows beyond doubt from the RP-HPLC quantification that the isolated bacterium has the ability to detoxify carbofuran (99% pure).Finally, the obtained results imply that the isolated strain of Enterobacter can be used as a potential and effective carbofuran degrader for bioremediation of contaminated sites through bioaugmentation. K E Y W O R D S antibiotic sensitivity, biodegradation, carbofuran, cytotoxicity, RP-HPLC How to cite this article: Ekram MA-E, Sarker I, Rahi MS, Rahman MA, Saha AK, Reza MA. Efficacy of soil-borne Enterobacter sp. for carbofuran degradation: HPLC quantitation of degradation rate.

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“… Aspergillus niger LDPE (20µm) 30 days 5.8 [ 44 ] 6. Rhodococcus ruber LDPE (0.2mm) 60 days 7.5 [ 45 ] 7. Pseudomonas putida LDPE (20µm) 120 days 9 [ 30 ] 8.…”

Section: Introductionmentioning

confidence: 99%

Biodegradation efficacy of soil inherent novel sp. Bacillus tropicus (MK318648) onto low density polyethylene matrix

Samanta

Datta²,

Halder

2020

J Polym Res

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The biodegradation of low density polyethylene (LDPE) was studied by employing a microbial strain isolated from the dumping site soil. The bacterium strain was identified as Bacillus tropicus (Gen Bank Accession no: MK318648) by 16S rRNA sequencing. The growth of the strain was observed on virgin LDPE during the biodegradation process. The change in properties of LDPE films before and after bacterial strain incubation was observed by FTIR, SEM, AFM, contact angle, mechanical and optical testing. Loss in mechanical properties and changes in optical properties of the polymer matrix was observed. Weight reduction by 10.15% and fall in the value of tensile strength, elongation at break, tear strength, Young's Modulus, hardness and stiffness to 8.59 MPa, 10.85 mm, 69.18 N, 272.36, 37.6 Shor D and 10,672.21 N/m respectively were observed after 40 days of incubation. The transparency and haze percentage were also changed to 93.7% and 18.6% respectively after the study period. The pH of the media was measured during incubation to evaluate the change due to formation of different extracellular and intracellular enzymes excreted by the strain. Hence, Bacillus tropicus could be an efficient microorganism to degrade 10-micron thickness LDPE films, thereby preventing its harmful impacts in the environment.

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Microbial degradation of low density polyethylene

Cited by 114 publications

References 42 publications

Biodegradation of Unpretreated Low-Density Polyethylene (LDPE) by Stenotrophomonas sp. and Achromobacter sp., Isolated From Waste Dumpsite and Drilling Fluid

Biodegradation of Unpretreated Low-Density Polyethylene (LDPE) by Stenotrophomonas sp. and Achromobacter sp., Isolated From Waste Dumpsite and Drilling Fluid

Efficacy of soil‐borne Enterobacter sp. for carbofuran degradation: HPLC quantitation of degradation rate

Biodegradation efficacy of soil inherent novel sp. Bacillus tropicus (MK318648) onto low density polyethylene matrix

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