Microbial degradation of polycyclic aromatic hydrocarbons in soils affected by the organic matrix of compost

Kastner, Marvin; Mahro, Bernd

doi:10.1007/s002530050615

Cited by 55 publications

(74 citation statements)

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“…Similar enzymes have also been detected in other bacteria and yeasts such as Acinetobacter calcoaceticus, Pseudomonas putida and Candida tropicalis, Candida, rugosa and Candida lipolytica respectively [17,18]. This however is in line with the findings of this study and supports the findings of Kastueur and Mahro [19], Onwurah [20] and Heidelberg et al [21] who reported that biodegradation pathways for each petroleum hydrocarbon or its derivatives are catalyzed by enzymes and cannot be degraded by a single strain of bacterium; hence it requires a consortium of microbes [22].…”

Section: Interactions Between Plant and Indigenous Microorganisms In supporting

confidence: 93%

Interaction Effects of Plants and Indigenous Micro-organisms on Degradation of N-Alkanes in Crude Oil Contaminated Agricultural Soil

Ogbulie¹,

Christopher²,

Nwanebu³

2015

J Ecosys Ecograph

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Agricultural soil samples from mapped out areas for the study were aseptically collected with sterile plastic sample containers and microbiologically analyzed to isolate autochthonous microbial flora. Seeds of four annual crops including Vigna unguiculata var unguiculata, Mucuna pruriens, Zea mays and T occidentalis used were planted on the test soil and polluted with Bonny light crude oil twenty eight (28) days after plant growth. Thirty days after pollution, soil samples were collected within the rhizosphere of the test plants and examined microbiologically to isolate persisting microorganisms in the polluted soil. The variation in degradation of n-alkanes was ascertained seven days after pollution using Gas chromatographic analysis on soil samples and compared with the control. The pre microbial lab analysis of the soil under the study revealed culturally, the presence of PenicillIum sp Aspergillus fumigatus, Aspergillus niger, Candida sp, Pseudomonas fluorescence, Acinetobacter baumanni, Bacillus mycoides, Klebsiella sp., Staphylococcus aureus and Escherichia coli whereas the absence of the last two isolates was observed during post microbial analyses. Results of the GC analysis on comparison to the control sample depict that plants kept in the greenhouse were able to degrade alkanes within the range of C 7 to C 12 and C 32 to C 40 while samples in the field degraded alkanes within the range C 7 to C 15 and C 36 to C 40 . M pruriens degraded C 13 . This study could be a promising tool in conversion of crude oil in contaminated agricultural soil to less toxic substances for enhanced remediation.

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Section: Interactions Between Plant and Indigenous Microorganisms In supporting

confidence: 93%

Interaction Effects of Plants and Indigenous Micro-organisms on Degradation of N-Alkanes in Crude Oil Contaminated Agricultural Soil

Ogbulie¹,

Christopher²,

Nwanebu³

2015

J Ecosys Ecograph

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“…Several studies have shown that the degradation of PAHs in contaminated soils is significantly higher after addition of compost (Kästner et al 1994;Wischmann and Steinhart 1997). Kästner and Mahro (1996) found that adding compost enhanced the removal of soil-associated PAHs and suggested that the presence of microorganisms capable of degrading natural humic substances were responsible for the co-metabolic degradation of the PAHs. Moreover, microbial activity has been shown to form bound residues in soil-compost system (Kästner et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Remediation of polycyclic aromatic hydrocarbon (PAH) contaminated soil through composting with fresh organic wastes

Zhang

Zhu

Houot

et al. 2011

Environ Sci Pollut Res

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Introduction Composting may enhance bioremediation of PAH-contaminated soils by providing organic substrates that stimulate the growth of potential microbial degraders. However, the influence of added organic matter (OM) together with the microbial activities on the dissipation of PAHs has not yet been fully assessed. Materials and methods An in-vessel composting-bioremediation experiment of a contaminated soil amended with fresh wastes was carried out. Four different experimental conditions were tested in triplicate during 60 days using laboratory-scale reactors: treatment S (100% soil), W (100% wastes), SW (soil/ waste mixture), and SWB (soil/waste mixture with inoculation of degrading microorganisms). Results and discussion A dry mass loss of 35±5% was observed in treatments with organic wastes during composting in all the treatments except treatment S. The dissipation of the 16 USEPA-listed PAHs was largely enhanced from no significant change to 50.5±14.8% (for SW)/63.7±10.0% (for SWB). More obvious dissipation was observed when fresh wastes were added at the beginning of composting to the contaminated soil, without significant difference between the inoculated and non-inoculated treatments. Phospholipid fatty acid (PLFA) profiling showed that fungi and G-bacteria dominated at the beginning of experiment and were probably involved in PAH dissipation. Subsequently, greater relative abundances of G+bacteria were observed as PAH dissipation slowed down. Conclusions The results suggest that improving the composting process with optimal organic compositions may be a feasible remediation strategy in PAH-contaminated soils through stimulation of active microbial populations.

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“…Organic mulch has many biopolymers, and among them lignin is known to have a high affinity and sorption capacity for nonionic organic compounds (Garbarini and Lion, 1986); they can also act as complex additive fertilizer for hydrocarbon degradation (Kastner and Mahro, 1996). However, in addition to the sorption capacity of supporting material in the biobarrier, microbial activity and biofilm formation play important roles in the performance of the permeable reactive biobarrier.…”

Section: Introductionmentioning

confidence: 99%

The monitoring of biofilm formation in a mulch biowall barrier and its effect on performance

Seo

Bishop

2008

Chemosphere

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Lab scale mulch-biofilm biowall barriers were constructed and tested to monitor the effect of biofilm formation on the performance of the biobarrier. Naphthalene, a two-ring polycyclic aromatic hydrocarbon (PAH), was used as the model compound. With column reactors, the amounts of viable naphthalene degraders and biofilm formation were monitored, as was the performance of the biobarrier.The sorption capacity of the mulch, the increase in biomass and the extracellular polymeric substance (EPS) content of the biofilm created a strong affinity for naphthalene and induced an increase in the number of slowly growing hydrocarbon degraders, resulting in a higher degradation rate and more stable PAH removal.Concentration profiles of pore water naphthalene and electron acceptors indicated that dissolved oxygen (DO) was preferentially used as the electron acceptor, and the greatest removal occurred at the inlet to the column reactor where DO was highest. However, when using nitrate as an alternative electron acceptor, both biofilm formation and continual degradation of naphthalene also occurred. Microprofiles of DO in the biofilm revealed that oxygen transport in the biofilm was limited, and there might be sequential utilization of nitrate for naphthalene removal in the anoxic zones of the biofilm. These results provide insight into the distribution of viable biomass and biofilm EPS production in engineered permeable reactive mulch biobarriers.

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Microbial degradation of polycyclic aromatic hydrocarbons in soils affected by the organic matrix of compost

Cited by 55 publications

References 0 publications

Interaction Effects of Plants and Indigenous Micro-organisms on Degradation of N-Alkanes in Crude Oil Contaminated Agricultural Soil

Interaction Effects of Plants and Indigenous Micro-organisms on Degradation of N-Alkanes in Crude Oil Contaminated Agricultural Soil

Remediation of polycyclic aromatic hydrocarbon (PAH) contaminated soil through composting with fresh organic wastes

The monitoring of biofilm formation in a mulch biowall barrier and its effect on performance

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