Microbial degradation pathways of the herbicide bentazone in filter sand used for drinking water treatment

Hedegaard, Mathilde Jørgensen; Prasse, Carsten; Albrechtsen, Hans‐Jørgen

doi:10.1039/c8ew00790j

Cited by 9 publications

(10 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our previous work has shown how a methanotrophic enrichment culture co-metabolically transformed bentazone to 6-OH-, 8-OH-, isopropyl-OH-, and di-OH-bentazone which accumulated in this enrichment culture (Hedegaard et al, 2018). However, these hydroxylated transformation products could not be detected during bentazone degradation in filter material from a waterworks, whereas the presence of further degraded transformation products demonstrated the importance of other metabolic pathways in these systems (Hedegaard et al, 2019). Thus, it is still unknown whether the initial bentazone degradation step in filter material depends on methane oxidation or other biological processes and to what extent this is valid in water treatment system.…”

Section: Introductionmentioning

confidence: 79%

“…In both approaches, bentazone removal in the filter material from Sjaelsø Plant II and Stenholt was much larger than in filter material from the remaining waterworks (Supplementary Figure S1). However, even though degradation clearly followed a linear trend through the entire period when measured by HPLC (Supplementary Figure S1A), the dissolved 14 C-activity did not decrease substantially after 1 day at Sjaelsø Plant II, or after 7 days at Stenholt waterworks (Supplementary Figure S1B), probably due to formation of transformation products (Hedegaard et al, 2019).…”

Section: Methane In the Raw Water And Removal Of Bentazone In Filter Materialsmentioning

confidence: 98%

“…Hence, the microbial community in the filter material from Stenholt only conducted the initial transformation of bentazone, while it was unable to mineralize the metabolites completely. In contrast, some of the initial bentazone was completely mineralized in filter material from Sjaelsø Plant II (Supplementary Figures S1A,C; Hedegaard et al, 2019).…”

Section: Methane In the Raw Water And Removal Of Bentazone In Filter Materialsmentioning

confidence: 99%

See 2 more Smart Citations

Importance of Methane Oxidation for Microbial Degradation of the Herbicide Bentazone in Drinking Water Production

Hedegaard

Schliemann-Haug

Milanović

et al. 2020

Front. Environ. Sci.

Self Cite

View full text Add to dashboard Cite

Bentazone is a herbicide, which is frequently detected in groundwater due to its mobility and persistence in aquifers. Groundwater is used as a drinking water source all over the world, and sustainable methods to remove pesticides at low concentrations are urgently needed since pesticide contaminations can adversely affect human health. The aim of this study was to investigate whether microbial bentazone degradation was associated with methane oxidation in full-scale drinking water treatment plants.To this end, we investigated bentazone biodegradation in microcosms with water and filter material from rapid sand filters, or biomass from aeration systems, and we investigated the statistical relation between the presence of methane and bentazone in groundwater abstraction wells. An array of evidence supported an association between bentazone degradation and methane oxidation in the biological treatment process. The biodegradation potential of bentazone was associated with the presence of methane in the raw water at 14 different water works. In contrast, no association was observed with any of the other investigated inorganic energy sources, e.g., ammonium. Addition of acetylene inhibited methane oxidation and the bentazone degradation in filter material from two investigated waterworks. Biomass from the aeration tanks degraded bentazone, but only while oxidizing methane. Bentazone removal rates and methane removal rates correlated significantly across all the experiments with biomass or filter material, with an overall transformation yield of 15 ± 1 × 10 −5 mole BTZ /mole CH4 . This demonstrated that the bentazone degradation was conducted by the same type of process in all the investigated communities, governed by methane oxidation. Furthermore, based on more than 10.000 water analyses from waterworks abstraction wells in Denmark, bentazone was detected significantly less frequent in wells with high methane concentrations (>1 mg/L) than in wells without methane. This suggests that biological treatment of bentazone contamination in drinking water may be achieved using methanotrophs.

show abstract

Section: Introductionmentioning

confidence: 79%

Section: Methane In the Raw Water And Removal Of Bentazone In Filter Materialsmentioning

confidence: 98%

Section: Methane In the Raw Water And Removal Of Bentazone In Filter Materialsmentioning

confidence: 99%

See 1 more Smart Citation

Importance of Methane Oxidation for Microbial Degradation of the Herbicide Bentazone in Drinking Water Production

Hedegaard

Schliemann-Haug

Milanović

et al. 2020

Front. Environ. Sci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Similarly, Hedegaard et al, and (2019) showed that 92% of bentazone, a widely used herbicide and often a recalcitrant groundwater contaminant, was degraded within 13 days in RSF. Moreover, Hedegaard et al (2019) suggested that various microbial groups were involved in the biodegradation: methanotrophs transformed bentazone to various hydroxylated TPs; thereafter, a diverse community was involved in the full degradation of TPs.…”

Section: Evaluation and Discussion On Omps Removal In Existing Rsf Symentioning

confidence: 98%

Harnessing biodegradation potential of rapid sand filtration for organic micropollutant removal from drinking water: A review

Wang

Ridder

Wal

et al. 2020

Critical Reviews in Environmental Science and Technology

View full text Add to dashboard Cite

A cost-effective approach for efficient organic micropollutants (OMPs) removal is to optimize existing infrastructure at drinking water treatment plants. A promising option is rapid sand filtration (RSF), as OMPs removal has been observed in this treatment technology. However, the mechanisms and pathways involved are not fully understood and strategies to optimize removal have yet to be thoroughly explored. Therefore, this article firstly described basic RSF functions that can support OMPs removal. OMPs can be removed by chemical and biological Mn/Fe oxides or degraded co-metabolically by ammonia oxidizing bacteria and methane oxidizing bacteria. In addition, heterotrophic bacteria can metabolically transform OMPs and their transformation products. Then, we reviewed current literatures described OMPs removal in RSF, showing biodegradation can contribute significantly to OMPs removal. Thereafter, we presented strategies to improve OMPs biodegradation, including bioaugmentation, optimizing hydraulic conditions by adjusting contact time and backwashing intensity, and adding biocarriers to retain biomass during rapid flow rates. Finally, we provided recommendations for further research towards optimizing and maintaining OMPs removal in RSF for safe drinking water production. This review therefore gives a critical evaluation of RSF-based technologies for OMPs removal from drinking water and provides recommendations for further improving OMPs removal in RSF.

show abstract

“…Biodegradation of bentazone in biological rapid sand filtration occurred mainly through three main biotransformation pathways: oxidation of the isopropyl moiety to the corresponding carboxylic acid, oxidation of the aromatic ring leading to ring cleavage and subsequent decarboxylation, and N-methylation followed by oxidation to a carboxylic acid [25].…”

Section: Introductionmentioning

confidence: 99%

Remediation of bentazone contaminated water by Trametes versicolor: Characterization, identification of transformation products, and implementation in a trickle-bed reactor under non-sterile conditions

García-Vara

Postigo

et al. 2021

Journal of Hazardous Materials

View full text Add to dashboard Cite

Bentazone, an herbicide widely applied in rice and cereal crops, is widespread in the aquatic environment. This study evaluated the capacity of Trametes versicolor to remove bentazone from water. The fungus was able to completely remove bentazone after three days at Erlenmeyer-scale incubation. Both laccase and cytochrome P450 enzymatic systems were involved in bentazone degradation. A total of 19 transformation products (TPs) were identified to be formed during the process. The reactions involved in their formation included hydroxylations, oxidations, methylations, N-nitrosation, and dimerization. A laccase mediated radical mechanism was proposed for TP formation. In light of the results obtained at the Erlenmeyer scale, a trickle-bed reactor with T. versicolor immobilized on pine wood chips was set up to evaluate its stability during bentazone removal under non-sterile conditions. After 30 days of sequencing batch operation, an average bentazone removal of 48 % was obtained, with a considerable contribution of adsorption onto the lignocellulosic support material. Bacterial contamination, which is the bottleneck in the implementation of fungal bioreactors, was successfully addressed by this particular system according to its maintained performance. This research is a pioneering step forward to the implementation of fungal bioremediation on a real scale.

show abstract

Microbial degradation pathways of the herbicide bentazone in filter sand used for drinking water treatment

Abstract: Three bentazone biodegradation pathways were identified in rapid sand filter material and relevant transformation products were less hazardous than bentazone.

Cited by 9 publications

References 27 publications

Importance of Methane Oxidation for Microbial Degradation of the Herbicide Bentazone in Drinking Water Production

Importance of Methane Oxidation for Microbial Degradation of the Herbicide Bentazone in Drinking Water Production

Harnessing biodegradation potential of rapid sand filtration for organic micropollutant removal from drinking water: A review

Remediation of bentazone contaminated water by Trametes versicolor: Characterization, identification of transformation products, and implementation in a trickle-bed reactor under non-sterile conditions

Contact Info

Product

Resources

About