Microbial Diversity Analysis in Spaceflight AIT Center

Yuan, Jing; Hong, YIN; Biao, ZHAO; Zhang, Lantao; Xi, QU; Xu, Kun

doi:10.11728/cjss2017.02.185

Cited by 1 publication

(3 citation statements)

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“…The diversity of the fungal assemblage in SAFs appears to be very much lower than that of the bacterial assemblage. Fungal spores and hyphae have been observed on fallout dust particles in SAFs (Checinska et al ., 2015; Yuan et al ., 2017; Mohan et al ., 2019). The isolates tended to be limited to common moulds and yeasts of the Ascomycota, such as Aspergillus, Cladosporium, Penicillium and Saccharomyces , and the Basidiomycota in the Tremellomycetes.…”

Section: Discussionmentioning

confidence: 99%

“…Of 26 genomes sequenced from SAF fungal isolates, 9 genera were detected comprising 13 species, with Aspergillus and Penicillium being most common (Chander et al ., 2022). From a Chinese SAF, 13 families of fungi were isolated, with Tremellomycetes being most common (Yuan et al ., 2017). Analyses of SAF fungal assemblages using molecular techniques gave similar results but also uncovered greater diversity.…”

Section: Discussionmentioning

confidence: 99%

“…A study of an SAF in Japan and the ISS by ITS sequencing suggested that ~50 OTUs of fungi were present in the SAF, with fewer than 10 detected for the ISS, including Cladosporium (90% of sequences), Alternaria and Aspergillus (Satoh et al ., 2011). The molecular analysis of samples from a Chinese SAF detected ~50 different species, including Debaryomyces and Pichia (Yuan et al ., 2017). Fungal assemblages in SAFs appear to be dominated by common Ascomycetes with relatively limited diversity.…”

Section: Discussionmentioning

confidence: 99%

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Succession of the fungal community of a spacecraft assembly clean room when enriched in brines relevant to Mars

Carte,

Chen,

Clark

et al. 2024

International Journal of Astrobiology

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Spacecraft can carry microbial contaminants from spacecraft assembly facilities (SAFs) to the cold arid surface of Mars that may confound life detection missions or disrupt native ecosystems. Dry hygroscopic sulphate and (per)chlorate salts on Mars may absorb atmospheric humidity and deliquesce at certain times to produce dense brines, potential sources of liquid water. Microbial growth is generally prohibited under the non-permissive condition of extremely low water activity in the frigid potential brines on Mars. Here we challenged the microbial community from samples of the Jet Propulsion Laboratory SAF with the extreme chemical conditions of brines relevant to Mars. Enrichment cultures in SP medium supplemented with 50% MgSO4 or 20% NaClO3 were inoculated from washes of SAF floor wipes. Samples were taken for each of the first four weeks and then at six months after inoculation to follow changes in the SAF microbial community under high salinity for long periods. Metagenomic DNA extracts of community samples were examined by Illumina sequencing of 18S rRNA gene sequences using fungal primers. The fungal assemblage during the first month of enrichment was predominantly common Ascomycetes, primarily Saccharomyete yeasts. Basidiomycetes were detected, mainly in the Microbotryomycetes and Tremellomycetes. Fungi were much less abundant in enrichment cultures at 50% MgSO4 than at 20% NaClO3. After 6 months of enrichment, few fungi remained. Microbes persisting from the JPL SAF microbial community in aged cultures enriched at extreme salinities might be the most capable of subsequently surviving and proliferating at the near surface of Mars. The SAF fungal assemblage did not survive and proliferate as well as the SAF bacterial community.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%