Microbial diversity in sugarcane ethanol production in a Brazilian distillery using a culture-independent method

Costa, Ohana Y.A.; Souto, Betulia M.; Tupinambá, Daiva Domenech; Bergmann, Jessica Carvalho; Kyaw, Cynthia Maria; Krüger, Ricardo Henrique; Barreto, Cristine Chaves; Quirino, Betânia Ferraz

doi:10.1007/s10295-014-1533-1

Cited by 50 publications

(61 citation statements)

References 61 publications

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“…In our study, OTUs (OTU-71 and OTU-317, Fig. 3e) belonging to these two genera were strongly favored by vinasse organic fertilization and as showed by Assis Costa et al (2015) they are also present in the ethanol production process. The same Acetobacter OTU favored by vinasse organic fertilization significantly explained N 2 O fluxes from soil with straw by the global fitted model.…”

Section: Discussionsupporting

confidence: 66%

“…Firmicutes, in particular lactic acids producers, are historically recognized as contaminants by the ethanol industry because their metabolites effectively inhibit yeast ethanol production (Muthaiyan et al, 2011). Thus, because most of the OTUs of Lactobacillus and Leuconostoc were not observed in treatments without vinasse application and Lactobacillus is the dominant taxa in vinasse (Assis Costa et al, 2015), vinasse residues might be the main source of these taxa to the soil (Fig. S6).…”

Section: Discussionmentioning

confidence: 99%

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Exploring soil microbial 16S rRNA sequence data to increase carbon yield and nitrogen efficiency of a bioenergy crop

et al. 2015

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Crop residues returned to the soil are important for the preservation of soil quality, health, and biodiversity, and they increase agriculture sustainability by recycling nutrients. Sugarcane is a bioenergy crop that produces huge amounts of straw (also known as trash) every year. In addition to straw, the ethanol industry also generates large volumes of vinasse, a liquid residue of ethanol production, which is recycled in sugarcane fields as fertilizer. However, both straw and vinasse have an impact on N 2 O fluxes from the soil. Nitrous oxide is a greenhouse gas that is a primary concern in biofuel sustainability. Because bacteria and archaea are the main drivers of N redox processes in soil, in this study we propose the identification of taxa related with N 2 O fluxes by combining functional responses (N 2 O release) and the abundance of these microorganisms in soil. Using a large-scale in situ experiment with ten treatments, an intensive gas monitoring approach, high-throughput sequencing of soil microbial 16S rRNA gene and powerful statistical methods, we identified microbes related to N 2 O fluxes in soil with sugarcane crops. In addition to the classical denitrifiers, we identified taxa within the phylum Firmicutes and mostly uncharacterized taxa recently described as important drivers of N 2 O consumption. Treatments with straw and vinasse also allowed the identification of taxa with potential biotechnological properties that might improve the sustainability of bioethanol by increasing C yields and improving N efficiency in sugarcane fields.

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Section: Discussionsupporting

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Exploring soil microbial 16S rRNA sequence data to increase carbon yield and nitrogen efficiency of a bioenergy crop

et al. 2015

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“…Although contaminants can enter the process through different ways, sugarcane is the major source and new strategies based on non-cultural techniques have been applied to access microbial communities that can affect industrial processes of bioethanol production 79, 80. Besides wild yeasts, bacteria compete for sugars and nutrients with starter yeast strains causing many problems, such as inhibition of fermentation, production of organic acids, reduction of industrial yields and flocculation 81, 82, 83…”

Section: Contaminating Yeast and Bacteriamentioning

confidence: 99%

Ethanol production in Brazil: a bridge between science and industry

Lopes

Paulillo

Godoy

et al. 2016

Brazilian Journal of Microbiology

319

174

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In the last 40 years, several scientific and technological advances in microbiology of the fermentation have greatly contributed to evolution of the ethanol industry in Brazil. These contributions have increased our view and comprehension about fermentations in the first and, more recently, second-generation ethanol. Nowadays, new technologies are available to produce ethanol from sugarcane, corn and other feedstocks, reducing the off-season period. Better control of fermentation conditions can reduce the stress conditions for yeast cells and contamination by bacteria and wild yeasts. There are great research opportunities in production processes of the first-generation ethanol regarding high-value added products, cost reduction and selection of new industrial yeast strains that are more robust and customized for each distillery. New technologies have also focused on the reduction of vinasse volumes by increasing the ethanol concentrations in wine during fermentation. Moreover, conversion of sugarcane biomass into fermentable sugars for second-generation ethanol production is a promising alternative to meet future demands of biofuel production in the country. However, building a bridge between science and industry requires investments in research, development and transfer of new technologies to the industry as well as specialized personnel to deal with new technological challenges.

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“…Studies have shown that the lactic acid produced by bacterial contaminants could reduce the ethanol production over time and result in economic losses (Costa et al . ).…”

Section: Resultsmentioning

confidence: 97%

“…LF, a heterofermentative organism, is the most predominant contaminant in industrial fermentation in Brazil (Costa et al . ) and can cause yeast flocculation capable of decreasing the bioethanol production (Dellias et al . ).…”

Section: Resultsmentioning

confidence: 99%

Nisin incorporation enhances the inactivation of lactic acid bacteria during the acid wash step of bioethanol production from sugarcane juice

Zhang

Holle

Kim

et al. 2019

Lett Appl Microbiol

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Bioethanol fermentation is continuously exposed to contamination by Lactic Acid Bacteria (LAB). In this study, the effect of adding nisin (250 mg l−1) to the acid wash on the viability of five bacterial contaminants were evaluated both alone and in co‐incubation with Saccharomyces cerevisiae. Additionally, fed‐batch fermentation was performed using an acid or acid/nisin wash for S. cerevisiae alone and cocultured with the LAB strains. Parameters such as ethanol production, sugar consumption and lactic acid production were monitored. Four model LAB were more susceptible to the acid/nisin wash than the acid wash, and were most susceptible when incubated with yeast. A fifth model LAB was very sensitive to both treatments regardless of the presence of yeast. The addition of nisin to the acid wash lowered the required time for adequate washing and resulted in a higher ethanol production (54·5 ± 0·1 g l−1) than the acid wash alone (52·6 ± 0·1 g l−1) in a subsequent fermentation. These results indicate the potential benefits of supplementing with nisin to improve the acid wash step of bioethanol fermentations. Significance and Impact of the Study Acid washing by the bioethanol fermentation industry reduces yeast efficiency and selects for contaminant bacteria that are resistant to acid treatments. This study demonstrates that the incorporation of nisin into the acid wash step results in a more potent removal of lactic acid bacteria while significantly shortening the length of time needed for the acid wash.

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Microbial diversity in sugarcane ethanol production in a Brazilian distillery using a culture-independent method

Cited by 50 publications

References 61 publications

Exploring soil microbial 16S rRNA sequence data to increase carbon yield and nitrogen efficiency of a bioenergy crop

Exploring soil microbial 16S rRNA sequence data to increase carbon yield and nitrogen efficiency of a bioenergy crop

Ethanol production in Brazil: a bridge between science and industry

Nisin incorporation enhances the inactivation of lactic acid bacteria during the acid wash step of bioethanol production from sugarcane juice

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