Microbial diversity of intestinal contents and mucus in yellow catfish (Pelteobagrus fulvidraco)

Wu, Shangong; Gao, Tianheng; Zheng, Yingzhen; Wang, Weiwei; Cheng, Yingyin; Wang, Guitang

doi:10.1016/j.aquaculture.2009.12.025

Cited by 200 publications

(169 citation statements)

References 53 publications

Supporting

Mentioning

133

Contrasting

Unclassified

Order By: Relevance

“…The fish stomach harbors characteristic microbiota, where Firmicutes is dominant, followed by Proteobacteria and Bacteroidetes and Fusobacteria. This bacterial community is markedly different from that of intestine, where Proteobacteria is predominant, followed by Fusobacteria and Firmicutes (Wu et al 2010). The finding that the difference in bacterial composition in the stomach and intestine is consistent with Zhou et al (2009), who concluded from the adult yellow grouper (Epinephelus awoara).…”

Section: Discussionsupporting

confidence: 81%

“…The result is similar to those of Ringø et al (2006), who disclosed that, when fed the soybean meal enriched diet, the TVC in the digesta of Atlantic cod (Gadus morhua L.) was higher than that in the mucus of the same segment. The TVCs in the stomach are lower than those revealed by Wu et al (2010) from the intestine. This result is congruent with Molinari et al (2003) and Navarrete et al (2009), who derived their conclusion from Nile tilapia Oreochromis niloticus and Atlantic salmon Salmo salar, respectively.…”

Section: Discussionmentioning

confidence: 53%

“…Due to its high market value, the aquaculture of this species has increased rapidly in recent years. Wu et al (2010) found that the fish intestine harbors characteristic intestinal microbiota, where Proteobacteria is predominant, followed by Fusobacteria, Firmicutes, Bacteroidetes and Actinobacteria. However, there is no information on the bacterial population structure of the stomach of this fish.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Characterization of bacterial community in the stomach of yellow catfish (Pelteobagrus fulvidraco)

Tian

Wang

et al. 2012

World J Microbiol Biotechnol

Self Cite

View full text Add to dashboard Cite

In this study, the traditional culture-based technique and the 16S rDNA sequencing method were used to investigate the characterization of bacterial community in the stomach contents and mucus of yellow catfish (Pelteobagrus fulvidraco). The culture-based technique disclosed that the average bacterial numbers in the gastric contents and mucus were 5.79 9 10 7 cfu/g (cfu: colony forming unit) and 1.89 9 10 5 cfu/g, respectively. Several different bacteria were obtained from gastric contents, including species from genera Bradyrhizobium, Phyllobacterium, Plesiomonas, Hafnia, Edwardsiella, Pseudomonas, and Bacillus. However, only two species were isolated from the gastric mucus, including species from genera Plesiomonas and Aeromonas. Forty-five phylotypes were observed from the 65 positive clones from the stomach contents (library SC); nineteen phylotypes were detected from the 45 clones from the stomach mucus (library SM). Further analyses revealed that the fish stomach harbored characteristic microbiota, where Firmicutes was dominant, followed by Proteobacteria and Bacteroidetes and Fusobacteria. This characterization of bacterial community is markedly different from that of the fish intestine, where Proteobacteria is predominant, followed by Fusobacteria and Firmicutes. Chloroflexi (1.5%) was only found in the library SC, while Actinobacteria (4.4%) was only found in the library SM, suggesting that microbiota of GI contents was quite different from that of GI mucus. In addition, several species of bacteria found in the stomach may be potentially opportunistic pathogens, indicating that fish digestive tract is a reservoir for many nosocomial pathogens.

show abstract

Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 53%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of bacterial community in the stomach of yellow catfish (Pelteobagrus fulvidraco)

Tian

Wang

et al. 2012

World J Microbiol Biotechnol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Cultured microbiota was isolated and enumerated as described by Wu et al (2010). Firstly, 1 g (wet weight) of the gut mucosa was homogenized for 10 min with a tissue grinder and vortexed severely in 9 ml volumes of sterile phosphate buffered (PBS; 0.1 mol l À1 , pH 7.2).…”

Section: Isolation and Enumeration Of Cultivable Bacteriamentioning

confidence: 99%

“…Recently, molecular methods, including the 16S rDNA clone library technique, have facilitated culture-independent studies and have improved the knowledge of gastrointestinal tracts of fish. Several studies showed that a molecular approach using 16S rDNA clone libraries could be a very powerful tool to analyse complex microbial communities (Holben, Williams, Saarinen, S€ arkilahti & Apajalahti 2002;Hovda, Lunestad, Fontanillas & Rosnes 2007;Kim, Brunt & Austin 2007;Wu, Gao, Zheng, Wang, Cheng & Wang 2010).…”

Section: Introductionmentioning

confidence: 99%

Diversity of autochthonous bacterial communities in the intestinal mucosa of grass carp (Ctenopharyngodon idellus) (Valenciennes) determined by culture-dependent and culture-independent techniques

Zhong

Wirth

et al. 2014

Aquac Res

Self Cite

View full text Add to dashboard Cite

Traditional culture-based technique and 16S rDNA sequencing method were used to investigate the mucosa-associated autochthonous microbiota of grass carp (Ctenopharyngodon idellus). Twenty-one phylotypes were detected from culturable microbiota, with Aeromonas, Shewanella, Lactococcus, Serratia, Brevibacillus, Delftia, Pseudomonas, Pantoea, Enterobacter, Buttiauxella and Yersinia as their closest relatives. Genomic DNA was directly extracted from the gut mucosa of C. idellus originating from six different geographical regions, and used to generate 609 random bacterial clones from six clone libraries and 99 archaeal clones from one library, which were grouped into 67 bacterial and four archaeal phylotypes. Sequence analysis revealed that the intestinal mucosa harboured a diversified bacterial microbiota, where Proteobacteria, Firmicutes and Bacteroidetes were dominant, followed by Actinobacteria, Verrucomicrobia and DeinococcusThermus. The autochthonous bacterial communities in the gut mucosa of fish from different aquatic environments were not similar (C s < 0.80), but c-Proteobacteria was a common bacterial class. In comparison to bacterial communities, the archaeal community obtained from one library consisted of Crenarchaeota and Euryarchaeota. These results demonstrate that molecular methods facilitate culture-independent studies, and that fish gut mucosa harbours a larger bacterial diversity than previously recognized. The grass carp intestinal habitat selects for specific bacterial taxa despite pronounced differences in host environments.

show abstract

Indigenous Lactic Acid Bacteria in Fish and Crustaceans

Merrifield

Balcázar

Daniels

et al. 2014

Aquaculture Nutrition

View full text Add to dashboard Cite

Microbial diversity of intestinal contents and mucus in yellow catfish (Pelteobagrus fulvidraco)

Cited by 200 publications

References 53 publications

Characterization of bacterial community in the stomach of yellow catfish (Pelteobagrus fulvidraco)

Characterization of bacterial community in the stomach of yellow catfish (Pelteobagrus fulvidraco)

Diversity of autochthonous bacterial communities in the intestinal mucosa of grass carp (Ctenopharyngodon idellus) (Valenciennes) determined by culture-dependent and culture-independent techniques

Indigenous Lactic Acid Bacteria in Fish and Crustaceans

Contact Info

Product

Resources

About