Microbial diversity of soil bacteria in agricultural field contaminated with heavy metals

Chien, Chih-Ching; Kuo, Yu-Mei; Chen, Chang-Chieh; Hung, Chun‐Wei; Yeh, Chihwei; Yeh, Weijen

doi:10.1016/s1001-0742(08)60056-x

Cited by 35 publications

(19 citation statements)

References 23 publications

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“…The structures of bacterial communities in heavymetal contaminated soils are represented mainly by Actinobacteria, Acidobacteria, Firmicutes and Proteobacteria (Kozdroj & van Elsas 2001;Ellis et al 2003;Zhang et al 2007;Chien et al 2008;Wu et al 2012). In these types of soils also the representatives of Gemmatimonadetes are often included in bacterial structures (Zhang et al 2007;Wu et al 2012).…”

Section: Resultsmentioning

confidence: 99%

“…These clones included, for example, Bacillus, Arthrobacter, Corynebacterium, Pseudomonas, Alcaligenes, Ralstonia and Burkholderia (Kozdroj & van Elsas 2001), Actinobacteria and Proteobacteria (α-, β-, γ-, and δ-subdivisions) (Ellis et al 2003), Acidobacteria, Gemmatimonadetes, Actinobacteria, Bacteroidetes, Chlamydiae, Chloroflexi, Fibrobacteres, and Proteobacteria (α-, β-, and δ-subdivisions) (Zhang et al 2007), Polyangium spp., Sphingomonas spp., Variovorax spp., Hafina spp., Clostridia, Acidobacteria, the enterics and some uncultured strains (Chien et al 2008), Proteobacteria (α-, β-, and γ-subdivisions), Planctomycetes, Firmicutes, Gemmatimonadetes, Acidobacteria, and Chloroflexi (Wu et al 2012). However, only limited information exists about structure of bacterial assemblages in soils contaminated by nickel, cobalt, zinc, and partly also by iron, copper and cadmium.…”

Section: Introductionmentioning

confidence: 99%

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Structure analysis of bacterial community and their heavy-metal resistance determinants in the heavy-metal-contaminated soil sample

et al. 2012

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In this study we performed the phylogenetic analysis of non-cultivable bacteria from anthropogenically disturbed soil using partial sequences of the 16S rRNA (16S rDNA) and the heavy-metal resistance genes. This soil sample contained high concentrations of nickel (2,109 mg/kg), cobalt (355 mg/kg) and zinc (177 mg/kg), smaller concentrations of iron (35.75 mg/kg) and copper (32.2 mg/kg), and also a trace amount of cadmium (<0.25 mg/kg). The 16S rDNA sequences from a total of 74 bacterial clones were distributed into four broad taxonomic groups, Acidobacteria, Actinobacteria, Bacteroidetes and Gemmatimonadetes, and some of them were unidentified. Comparing our clone sequences with those from the GenBank database, only 9 clones displayed high similarity to known bacteria belongig to actinomycetes; others were identified as uncultured ones. Among clones evidently Actinobacteria predominated. Sixteen clones from soil sample carried only the nccA-like heavy-metal-resistance genes and all sequences showed too low similarity to known proteins encoded by these genes. However, our results suggested that the heavy-metal-contaminated soil is able to present very important reservoir of the new and until now unknown partly bacteria, partly heavy-metal-resistance determinants and their products. Bacteria and nccA-like genes identified in this study could represent the objects of interest as bioremediation agents because they can be potentially used in different transformation and immobilization processes.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Structure analysis of bacterial community and their heavy-metal resistance determinants in the heavy-metal-contaminated soil sample

et al. 2012

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“…The principal effects of metal toxicities in the soil include reduction in species diversity, microbial biomass, increase in rate of respiration, and changes in microbial community structure (Liao and Xie, 2007;Crowley, 2008). A number of studies have also reported rise in metal tolerant populations in metal polluted soils (Kanazawa and Mori, 1996;Ellis et al, 2003;Almas et al, 2004;Chien et al, 2008).…”

Section: Contamination On Soil Microorganismsmentioning

confidence: 99%

Effects of Metal and Metalloid Contamination on Microbial Diversity and Activity in Agricultural Soils

Tipayno¹,

Chauhan²,

Woo³

et al. 2011

Korean Journal of Soil Science and Fertilizer

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The continuous increase in the production of metals and their subsequent release into the environment has lead to increased concentration of these elements in agricultural soils. Because microbes are involved in almost every chemical transformations taking place in the soil, considerable attention has been given to assessing their responses to metal contaminants. Short-term and long-term exposures to toxic metals have been shown to reduce microbial diversity, biomass and activities in the soil. Several studies show that microbial parameters like basal respiration, metabolic quotient, and enzymatic activities, including those of oxidoreductases and those involved in the cycle of C, N, P and other elements, exhibit sensitivity to soil metal concentrations. These have been therefore, regarded as good indices for assessing the impact of metal contaminants to the soil. Metal contamination has also been extensively shown to decrease species diversity and cause shifts in microbial community structure. Biochemical and molecular techniques that are currently being employed to detect these changes are continuously challenged by several limiting factors, although showing some degree of sensitivity and efficiency. Variations and inconsistencies in the responses of bioindicators to metal stress in the soil can also be explained by differences in bioavailability of the metal to the microorganisms. This, in turn, is influenced by soil characteristics such as CEC, pH, soil particles and other factors. Therefore, aside from selecting the appropriate techniques to better understand microbial responses to metals, it is also important to understand the prevalent environmental conditions that interplay to bring about observed changes in any given soil parameter.

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“…The appreciable diversity of microorganisms in environments contaminated with xenobiotics and heavy metals suggests that microorganisms not only have adapted to these adverse niches but have flourished in them [27][28][29][30][31]. We aim to isolate microorganisms that can tolerate both the heavy metal Cd and the metalloid oxyanion tellurite as well as demonstrate TeO 2À 3 detoxification.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of an environmental cadmium‐ and tellurite‐resistant Pseudomonas strain

Chien

Jiang

Tsai

2011

Enviro Toxic and Chemistry

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A Pseudomonas strain (TeU), resistant to tellurite (TeO(2)(3)(-) and cadmium (Cd(2+)) ions, was isolated from heavy-metal-contaminated sediments by enrichment. Black precipitates, presumably the product of the reduction of tellurite, such as tellurium, occurred in cultures of the isolate after growth in medium containing tellurite. Quantitative determination of the TeO(2)(3)(-) concentration in the liquid culture demonstrated a decreased concentration of tellurite (to less than 100 µM) from initial concentrations of approximately 1,000 µM within 24 h of growth. Strain TeU was resistant to TeO(2)(3)(-) and Cd(2+) concentrations as high as 2,000 µM and 500 µM, respectively. Transposon mutagenesis of strain TeU resulted in mutants exhibiting Cd(2+) sensitivity (Strain BU21) and one with decreased ability to reduce tellurite (strain AU08). Strain BU21 was less tolerant to Cd(2+) (100 µM) compared with the wild-type strain TeU (500 µM) but was still able to reduce tellurite to 80% of that of strain TeU. Although strain AU08 possesses the ability for Cd(2+) resistance, it reduced less than 20% of the initial concentrations of tellurite compared with strain TeU. Genes encoding an HflKC complex and a putative metallopeptidase were associated with the bacterium's capacity for tellurite reduction and Cd resistance, respectively. The ability to reduce tellurite therefore may not be necessary for this bacterium's heavy metal and metalloid tellurite resisting ability.

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Microbial diversity of soil bacteria in agricultural field contaminated with heavy metals

Cited by 35 publications

References 23 publications

Structure analysis of bacterial community and their heavy-metal resistance determinants in the heavy-metal-contaminated soil sample

Structure analysis of bacterial community and their heavy-metal resistance determinants in the heavy-metal-contaminated soil sample

Effects of Metal and Metalloid Contamination on Microbial Diversity and Activity in Agricultural Soils

Isolation and characterization of an environmental cadmium‐ and tellurite‐resistant Pseudomonas strain

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