Microbial diversity of thermophiles with biomass deconstruction potential in a foliage‐rich hot spring

Lee, Li Sin; Goh, Kian Mau; Chan, Chia Sing; Tan, Geok Yuan Annie; Yin, Wai‐Fong; Chong, Chun Shiong; Chan, Kok-Gan

doi:10.1002/mbo3.615

Cited by 29 publications

(17 citation statements)

References 72 publications

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“…For example, some bacteria in module I are involved in plant litter degradation. Fungal genera Mortierella 67 and Acremonium 68 , as well as bacterial genera Roseiflexus 69 and Rubrobacter 70 that contain various lignocelluloses, xylanases, and glycoside hydrolases, may play important roles in cellulose, hemicellulose, or lignin breakdown. The main taxa in module II may be involved in biogeochemical C- and N-cycles.…”

Section: Discussionmentioning

confidence: 99%

Habitat filtering shapes the differential structure of microbial communities in the Xilingol grassland

Yang

Wang

Cui

et al. 2019

Sci Rep

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The spatial variability of microorganisms in grasslands can provide important insights regarding the biogeographic patterns of microbial communities. However, information regarding the degree of overlap and partitions of microbial communities across different habitats in grasslands is limited. This study investigated the microbial communities in three distinct habitats from Xilingol steppe grassland, i.e. animal excrement, phyllosphere, and soil samples, by Illumina MiSeq sequencing. All microbial community structures, i.e. for bacteria, archaea, and fungi, were significantly distinguished according to habitat. A high number of unique microorganisms but few coexisting microorganisms were detected, suggesting that the structure of microbial communities was mainly regulated by species selection and niche differentiation. However, the sequences of those limited coexisting microorganisms among the three different habitats accounted for over 60% of the total sequences, indicating their ability to adapt to variable environments. In addition, the biotic interactions among microorganisms based on a co-occurrence network analysis highlighted the importance of Microvirga, Blastococcus, RB41, Nitrospira, and four norank members of bacteria in connecting the different microbiomes. Collectively, the microbial communities in the Xilingol steppe grassland presented strong habitat preferences with a certain degree of dispersal and colonization potential to new habitats along the animal excrement- phyllosphere-soil gradient. This study provides the first detailed comparison of microbial communities in different habitats in a single grassland, and offers new insights into the biogeographic patterns of the microbial assemblages in grasslands.

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Section: Discussionmentioning

confidence: 99%

Habitat filtering shapes the differential structure of microbial communities in the Xilingol grassland

Yang

Wang

Cui

et al. 2019

Sci Rep

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“…UICC B-76 and Paenibacillus cisolokensis LC2-13A T , respectively. The plant litter degrading ability of the thermophilic bacteria from the phylum Firmicutes also reported by Lee et al (2018). They found that the dominant phyla in the microbiota of degraded foliage from the deeper part of hot spring were Firmicutes and Proteobacteria.…”

Section: Screening Of Xylan-degrading Abilitymentioning

confidence: 54%

“…Thus, bacteria that possess xylanase could use it as an organic nutrient. According to Lee et al (2018), Actinobacteria was one of the classes that contributed to almost 69% of the total population in the degraded foliage microbiota from foliage-rich hot spring in Sungai Klah, Malaysia, based on metagenomic analysis. Our previous studies, Mawarid et al (2016) and Yokota et al (2016), have reported the xylan-degrading ability of thermophilic bacteria from the phylum Firmicutes isolated from litter in Cisolok geyser, e.g.…”

Section: Screening Of Xylan-degrading Abilitymentioning

confidence: 99%

Xylan-degrading ability of thermophilic Actinobacteria from soil in a geothermal area

Rachmania¹,

Ningsih²,

Setyaningsih³

et al. 2019

Biodiversitas

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Abstract. Rachmania MK, Ningsih F, Setyaningsih PP, Syafitri WA, Sari DCAF, Yabe S, Yokota A, Oetari A, Sjamsuridjal W. 2020. Xylan-degrading ability of thermophilic Actinobacteria isolated from soil in a geothermal area. Biodiversitas 21: 144-154. This study was conducted to obtain the potential isolates of xylan-degrading thermophilic Actinobacteria. Seventeen isolates were obtained from the soil samples collected from Cisolok geysers in West Java, Indonesia. These isolates were screened for their xylan-degrading ability on minimal medium with the addition of 0.5% xylan as substrate, and were incubated at various temperatures for 7 days. A total of 15, 14, 4, and 3 isolates showed a xylan-degrading ability at 45°C, 50°C, 55°C, and 60°C, respectively. The three isolates (SL1-2-R-2, SL1-2-R-3, and SL1-2-R-4) that showed xylan-degrading ability at 60°C on 0.5% xylan were further tested by using minimal medium with the addition of 0.1% RBB-xylan as a substrate. The results showed that these isolates were also able to utilized RBB-xylan at 45 to 60°C. The xylanolytic activity of the crude enzymes from the three isolates on minimal medium containing 0.5% xylan or 0.1% RBB-xylan as substrates, indicated that these isolates were able to produce extracellular xylanase. This study revealed that the soil of Cisolok geysers served as an ideal source for finding the thermophilic Actinobacteria which produced xylanase at high temperatures.

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“…Their quick reaction time, thermostability, and decreased risk of contamination are an adeptness of thermophilic microorganisms and their enzymes. Thus, a good source for isolating efficient biomass degrading thermophiles and thermozymes was a hot spring (Lee et al, 2018).…”

Section: Relative Abundancementioning

confidence: 99%

The Chemical Characteristic and Microbial Diversity of the Hot Spring at Phusang National Park

Bumrungthai

Duangjit

Somsuwan³

et al. 2019

Environ. Nat. Resour. J.

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The robustness of Phusang's warm waterfall is regionally recognized as the only one with a temperature range of 35-36 °C which makes it an outstanding place to visit in Thailand. Surprisingly, Phusang waterfall originates from the Phusang warm pool (hot spring). However, data about the bacterial community and characteristics of this water are still obscure. Therefore, this study investigated the bacterial community and characteristics of water in the Phusang hot spring. Tests to determine its physical characteristics such as pH, color and turbidity were performed. Trace elements such as sodium, bicarbonates, iron, and fluoride were detected as chemical characteristics. The biological properties were verified by 16S ribosomal RNA sequencing. Illumina metagenomic analysis was directly demonstrated from the water after DNA extraction via a membrane filtration pore of 0.45 µm. The range of pH, color and turbidity of water from the Phusang hot spring was 7.33-7.53, 0.05-0.18 Pt.Co and 9.55-10.91 NTU, respectively. The biological study of microorganisms found less than 300 CFU/mL. Coliform bacteria such as Staphylococcus aureus and other examples such as Aeromonas veronii, Acinetobacter sp. Neisseriaceae bacterium were abundant. Shotgun metagenomic sequencing defined the phylum as Proteobacteria (84%), Bacteroidetes (13%), Cyanobacteria (1%), and unclassified (2%). Moreover, the amount of sodium and strontium detected was 6.00-7.52 and 1.40-1.58 mg/L respectively. These studies show that a high abundance of Proteobacteria were present in samples from this hot spring. Phusang hot spring has been classified as having low mineral content water.

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Microbial diversity of thermophiles with biomass deconstruction potential in a foliage‐rich hot spring

Cited by 29 publications

References 72 publications

Habitat filtering shapes the differential structure of microbial communities in the Xilingol grassland

Habitat filtering shapes the differential structure of microbial communities in the Xilingol grassland

Xylan-degrading ability of thermophilic Actinobacteria from soil in a geothermal area

The Chemical Characteristic and Microbial Diversity of the Hot Spring at Phusang National Park

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