Microbial Ecology of Methanotrophy in Streams Along a Gradient of CH4 Availability

Bagnoud, Alexandre; Pramateftaki, Paraskevi; Bogard, Matthew J.; Battin, Tom J.; Peter, Hannes

doi:10.3389/fmicb.2020.00771

Cited by 8 publications

(4 citation statements)

References 49 publications

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“…Methylomirabilis sp. bacteria are restricted in using NO 2 − as an EA in CH 4 oxidation and have been reported to have a high affinity for CH 4 ( 10 , 28 ), Methylococcales are potentially capable of coupling CH 4 oxidation with fermentation and with reduction of a variety of EAs (e.g., O 2 , NO 3 − , NO 2 − , Fe 3+ , and organic EAs) and have a low affinity for CH 4 ( 11 – 19 , 26 , 27 ). This gives Methylococcales advantage over Ca .…”

Section: Resultsmentioning

confidence: 99%

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Organic matter lability modifies the vertical structure of methane-related microbial communities in lake sediments

Rissanen,

Jilbert,

Simojoki

et al. 2023

Microbiol Spectr

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Eutrophication increases the input of labile, algae-derived, organic matter (OM) into lake sediments. This potentially increases methane (CH 4 ) emissions from sediment to water through increased methane production rates and decreased methane oxidation efficiency in sediments. However, the effect of OM lability on the structure of methane oxidizing (methanotrophic) and methane producing (methanogenic) microbial communities in lake sediments is still understudied. We studied the vertical profiles of the sediment and porewater geochemistry and the microbial communities (16S rRNA gene amplicon sequencing) at five profundal stations of an oligo-mesotrophic, boreal lake (Lake Pääjärvi, Finland), varying in surface sediment OM sources (assessed via sediment C:N ratio). Porewater profiles of methane, dissolved inorganic carbon (DIC), acetate, iron, and sulfur suggested that sites with more autochthonous OM showed higher overall OM lability, which increased remineralization rates, leading to increased electron acceptor (EA) consumption and methane emissions from sediment to water. When OM lability increased, the abundance of anaerobic nitrite-reducing methanotrophs ( Candidatus Methylomirabilis) relative to aerobic methanotrophs ( Methylococcales ) in the methane oxidation layer of sediment surface decreased, suggesting that Methylococcales were more competitive than Ca . Methylomirabilis under decreasing redox conditions and increasing methane availability due to their more diverse metabolism (fermentation and anaerobic respiration) and lower affinity for methane. Furthermore, when OM lability increased, the abundance of methanotrophic community in the sediment surface layer, especially Ca . Methylomirabilis, relative to the methanogenic community decreased. We conclude that increasing input of labile OM, subsequently affecting the redox zonation of sediments, significantly modifies the methane producing and consuming microbial community of lake sediments. IMPORTANCE Lakes are important natural emitters of the greenhouse gas methane (CH 4 ). It has been shown that eutrophication, via increasing the input of labile organic matter (OM) into lake sediments and subsequently affecting the redox conditions, increases methane emissions from lake sediments through increased sediment methane production rates and decreased methane oxidation efficiency. However, the effect of organic matter lability on the structure of the methane-related microbial communities of lake sediments is not known. In this study, we show that, besides the activity, also the structure of lake sediment methane producing and consuming microbial community is significantly affected by changes in the sediment organic matter lability.

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Section: Resultsmentioning

confidence: 99%

“…capability for fermentation and anaerobic respiration of various EAs as explained above) than Ca . Methylomirabilis ( 12 , 15 , 17 , 18 , 26 – 28 ). Hence, it could be expected that changes in the OM quality of lake sediments affect differently the abundances of MOB and Ca .…”

Section: Introductionmentioning

confidence: 99%

Organic matter lability modifies the vertical structure of methane-related microbial communities in lake sediments

Rissanen,

Jilbert,

Simojoki

et al. 2023

Microbiol Spectr

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“…Lower In freshwaters, a large fraction of CH4 in the aquatic food network exists as a carbon source which was oxidized by bacteria, can reduce CH4 entering the water by diffusion (Jones and Grey, 2011;Taipale et al, 2011;Frossard et al, 2015;Sawakuchi et al, 2016;Matoušů et al, 2019;Thottathil et al, 2019;Saarela et al, 2020), which may change CH4 dynamics in water. Previous studies reported that the methane oxidizing bacteria (MOB) can survive at oxic-anoxic interfaces in freshwater systems, and CH4 oxidation was frequently found in environments with high CH4 and DO concentrations (Bagnoud et al, 2020;Reis et al, 2020;Bai et al, 2021 (Whiticar and Faber, 1986;Conrad, 1999;Bastviken et al, 2002;Lima, 2005). The magnitude of δ 13 C-CH4 by MOB significantly depends on environmental conditions, so the fractionation factor (α) caused by microbial CH4 oxidation shows a large range of 1.003 to 1.039 (Templeton et al, 2006).…”

Section: Spatial and Temporal Variations In Ch4 And Co2 Concentrations δ 13 C-ch4mentioning

confidence: 99%

Methane accumulation affected by particulate organic carbon in upper Yangtze deep valley dammed cascade reservoirs, China

Zhang

et al. 2021

Preprint

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Abstract. Methane (CH4) emissions from freshwaters to the atmosphere have a profound impact on global atmospheric greenhouse gas (GHG) concentrations. Anthropogenic footprints such as dam construction and reservoir operation significantly changed the fate and transport of CH4 in freshwaters. The type of particulate organic carbon (POC) in reservoirs is a critical factor controlling CH4 production and emissions. However, little is known of how reservoir operation mediates the distribution of POC and regulates CH4 accumulation in cascade hydroelectric reservoirs. Here, spatial and temporal variations in POC and CH4 were explored in the Xiluodu (XLD) and Xiangjiaba (XJB) reservoirs which are deep valley dammed cascade reservoirs located in the main channel of the upper Yangtze River. Based on the δ13C-POC and N / C mole ratios of particulate organic matter, the results of multi-endmember stable isotope mixing models by a Bayesian model show that terrestrial POC and autochthonous POC accounted for approximately 56 ± 19 % and 42 ± 19 % (SD, n = 181) of POC, respectively. CH4 concentrations and δ13C-CH4 in the cascade reservoirs were potentially influenced by CH4 oxidation. Together with other physicochemical parameters and structural equation model, these results suggested that the input of terrestrial POC was dominantly influenced by water level variations and flow regulation due to reservoir operation. The cumulative effect of POC caused by cascade reservoirs was not apparent at a bimonthly scale. Terrestrial POC was more likely to dominate CH4 accumulation in cascade reservoirs under reservoir operation.

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“…Lower In freshwaters, a large fraction of CH4 in the aquatic food network exists as a carbon source which was oxidized by bacteria, can reduce CH4 entering the water by diffusion (Jones and Grey, 2011;Taipale et al, 2011;Frossard et al, 2015;Sawakuchi et al, 2016;Matoušů et al, 2019;Thottathil et al, 2019;Saarela et al, 2020), which may change CH4 dynamics in water. Previous studies reported that the methane oxidizing bacteria (MOB) can survive at oxic-anoxic interfaces in freshwater systems, and CH4 oxidation was frequently found in environments with high CH4 and DO concentrations (Bagnoud et al, 2020;Reis et al, 2020;Bai et al, 2021). Moreover, the δ 13 C values of biogenic methane during methanogenesis were very low, such as methanogenesis by acetate fermentation in freshwaters, and the δ 13 C-CH4 was then gradually elevated during CH4 oxidation due to 13 C enrichment in residual CH4 (Whiticar and Faber, 1986;Conrad, 1999;Bastviken et al, 2002;Lima, 2005).…”

Section: Particulate Organic Matter and Stable Isotopic Signaturesmentioning

confidence: 99%

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2021

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Microbial Ecology of Methanotrophy in Streams Along a Gradient of CH4 Availability

Cited by 8 publications

References 49 publications

Organic matter lability modifies the vertical structure of methane-related microbial communities in lake sediments

Organic matter lability modifies the vertical structure of methane-related microbial communities in lake sediments

Methane accumulation affected by particulate organic carbon in upper Yangtze deep valley dammed cascade reservoirs, China

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