Microbial electrosynthesis with <i>Clostridium ljungdahlii</i> benefits from hydrogen electron mediation and permits a greater variety of products

Boto, Santiago T.; Bardl, Bettina

doi:10.1039/d3gc00471f

Cited by 38 publications

(17 citation statements)

References 83 publications

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“…As with S. ovata, the question of whether or not C. ljundahlii can directly accept electrons from electrodes remains unresolved 13,58 . Microbial electrosynthesis with C. ljungdahlii benefits from H 2 mediating electron transfer between electrodes and cells 39,58 . C. ljundahlii readily produced acetate with pure Fe 0 as the electron donor (Figure 4B).…”

Section: Electrobiocorrosion With S Ovata But Not Clostridium Ljundahliimentioning

confidence: 99%

Electrobiocorrosion by microbes without outer‐surface cytochromes

Holmes,

Woodard,

Smith

et al. 2024

mLife

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Anaerobic microbial corrosion of iron‐containing metals causes extensive economic damage. Some microbes are capable of direct metal‐to‐microbe electron transfer (electrobiocorrosion), but the prevalence of electrobiocorrosion among diverse methanogens and acetogens is poorly understood because of a lack of tools for their genetic manipulation. Previous studies have suggested that respiration with 316L stainless steel as the electron donor is indicative of electrobiocorrosion, because, unlike pure Fe0, 316L stainless steel does not abiotically generate H2 as an intermediary electron carrier. Here, we report that all of the methanogens (Methanosarcina vacuolata, Methanothrix soehngenii, and Methanobacterium strain IM1) and acetogens (Sporomusa ovata and Clostridium ljungdahlii) evaluated respired with pure Fe0 as the electron donor, but only M. vacuolata, Mx. soehngenii, and S. ovata were capable of stainless steel electrobiocorrosion. The electrobiocorrosive methanogens required acetate as an additional energy source in order to produce methane from stainless steel. Cocultures of S. ovata and Mx. soehngenii demonstrated how acetogens can provide acetate to methanogens during corrosion. Not only was Methanobacterium strain IM1 not capable of electrobiocorrosion, but it also did not accept electrons from Geobacter metallireducens, an effective electron‐donating partner for direct interspecies electron transfer to all methanogens that can directly accept electrons from Fe0. The finding that M. vacuolata, Mx. soehngenii, and S. ovata are capable of electrobiocorrosion, despite a lack of the outer‐surface c‐type cytochromes previously found to be important in other electrobiocorrosive microbes, demonstrates that there are multiple microbial strategies for making electrical contact with Fe0.

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Section: Electrobiocorrosion With S Ovata But Not Clostridium Ljundahliimentioning

confidence: 99%

Electrobiocorrosion by microbes without outer‐surface cytochromes

Holmes,

Woodard,

Smith

et al. 2024

mLife

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“…In our study, A. wieringae exhibited the highest H2 consumption rate under our experimental conditions, followed by S. ovata and the two Clostridium strains (Table 2, Figure 4). Numerous studies already explored the capabilities of C. ljungdahlii and C. autoethanogenum as catalysts in gas conversion technologies [48][49][50][51][52] , because their high conversion rates. Maybe less known, but also A. wieringae has already been recognized for its high conversion rates on C₁ carbon sources 53,54 .…”

Section: First-order H2 Consumption Rate Coefficients Differ Up To Si...mentioning

confidence: 99%

H₂consumption by various acetogenic bacteria follows first-order kinetics up to H₂saturation

Muñoz-Duarte,

Chakraborty,

Grøn

et al. 2024

Preprint

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Acetogenic bacteria play an important role in various environmental and biotechnological processes, because of their autotrophic metabolism converting carbon dioxide with molecular hydrogen (H2) as electron donor into acetate. The main factor limiting acetogenesis is often H2. Assessing the potential of acetogens in environmental and biotechnological processes thus requires insights into their H2 consumption kinetics. In this study, initial H2 consumption rates at a range of different initial H2 concentrations were measured for three different acetogens. Interesting, for all three strains, H2 consumption was found to follow first-order kinetics, i.e. the H2 consumption rate increased linearly with the dissolved H2 concentration up to almost saturated H2 levels. This contradicts Monod kinetics, which is commonly assumed for acetogens. The obtained first-order rate coefficients (k1) were further validated by fitting first-order kinetics on previous time-course experimental results. The latter method was also used to determine the k1 value of five additional acetogens strains. Biomass specific first-order rate coefficients were found to vary up to six-fold, with the highest k1 for Acetobacterium wieringae and the lowest for Sporomusa sphaeroides. Overall, our results demonstrate the importance of the dissolved H2 concentration to understand the rate of acetogenesis in environmental and biotechnological settings.

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“…As with S. ovata, the question of whether or not Clostridium ljundahlii can directly accept electrons from electrodes is unresolved (13,62). Microbial electrosynthesis with Clostridium ljungdahlii benefits from H 2 mediating electron transfer between electrodes and cells (40,62). C. ljundahlii readily produced acetate with pure Fe 0 as the electron donor (Figure 4B).…”

Section: Electrobiocorrosion With S Ovata But Not C Ljundahliimentioning

confidence: 99%

Electrobiocorrosion by Microbes without Outer-Surface Cytochromes

Holmes

Woodard

Smith

et al. 2023

Preprint

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Anaerobic microbial corrosion of iron-containing metals causes extensive economic damage. Some microbes are capable of direct metal-to-microbe electron transfer (electrobiocorrosion), but the prevalence of electrobiocorrosion among diverse methanogens and acetogens is poorly understood because of a lack of tools for their genetic manipulation. Previous studies have suggested that respiration with 316L stainless steel as the electron donor is indicative of electrobiocorrosion because, unlike pure Fe0, 316L stainless steel does not abiotically generate H2as an intermediary electron carrier. Here we report that all of the methanogens (Methanosarcina vacuolata,Methanothrix soehngenii, andMethanobacteriumstrain IM1) and acetogens (Sporomusa ovata,Clostridium ljungdahlii) evaluated respired with pure Fe0as the electron donor, but onlyM. vacuolata,Mx soehngenii, andS. ovatawere capable of stainless steel electrobiocorrosion. The electrobiocorrosive methanogens required acetate as an additional energy source in order to produce methane from stainless steel. Co-cultures ofS. ovataandMx. soehngeniidemonstrated how acetogens can provide acetate to methanogens during corrosion. Not only wasMethanobacteriumstrain IM1 not capable of electrobiocorrosion, but it also did not accept electrons fromGeobacter metallireducens, an effective electron- donating partner for direct interspecies electron transfer to all methanogens that can directly accept electrons from Fe0. The finding thatM. vacuolata,Mx. soehngenii, andS. ovataare capable of electrobiocorrosion, despite a lack of the outer-surfacec-type cytochromes previously found to be important in other electrobiocorrosive microbes, demonstrates that there are multiple microbial strategies for making electrical contact with Fe0.Impact StatementUnderstanding how anaerobic microbes receive electrons from Fe0is likely to lead to novel strategies for mitigating the corrosion of iron-containing metals, which has an enormous economic impact. Electrobiocorrosion, is a relatively recently recognized corrosion mechanism. It was previously demonstrated in pure cultures when Fe0oxidation was inhibited by deletion of genes for outer-surfacec-type cytochromes known to be involved in other forms of extracellular electron exchange. However, many methanogens and acetogens lack obvious outer-surface electrical connections and are difficult to genetically manipulate. The study reported here provides an alternative approach to evaluating whether microbes are capable of electrobiocorrosion that does not require genetic manipulation. The results indicate thatMethanobacteriumstrain IM1, is not electrobiocorrosive, in contrast to previous speculation. However, some methanogens and acetogens without known outer-surfacec-type cytochromes do appear to be capable of electrobiocorrosion, suggesting that this corrosion mechanism may be more widespread than previously thought.

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Microbial electrosynthesis with Clostridium ljungdahlii benefits from hydrogen electron mediation and permits a greater variety of products

Abstract: Hydrogen-mediated microbial electrosynthesis with Clostridium ljungdahlii enabled the metabolic flux diversification from acetate to glycine and ethanolamine.

Cited by 38 publications

References 83 publications

Electrobiocorrosion by microbes without outer‐surface cytochromes

Electrobiocorrosion by microbes without outer‐surface cytochromes

H₂consumption by various acetogenic bacteria follows first-order kinetics up to H₂saturation

Electrobiocorrosion by Microbes without Outer-Surface Cytochromes

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Microbial electrosynthesis with Clostridium ljungdahlii benefits from hydrogen electron mediation and permits a greater variety of products

Abstract: Hydrogen-mediated microbial electrosynthesis with Clostridium ljungdahlii enabled the metabolic flux diversification from acetate to glycine and ethanolamine.

Cited by 38 publications

References 83 publications

Electrobiocorrosion by microbes without outer‐surface cytochromes

Electrobiocorrosion by microbes without outer‐surface cytochromes

H2consumption by various acetogenic bacteria follows first-order kinetics up to H2saturation

Electrobiocorrosion by Microbes without Outer-Surface Cytochromes

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H₂consumption by various acetogenic bacteria follows first-order kinetics up to H₂saturation