Microbial Hydrolytic Enzymes: Powerful Weapons Against Insect Pests

Lopes, Fernanda Cortez; Martinelli, Anne Helene Souza; John, Elisa B. O.; Ligabue-Braun, Rodrigo

doi:10.1007/978-3-030-67231-7_1

Cited by 4 publications

(4 citation statements)

References 175 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar symptoms have earlier been reported in P. xylostella and S. litura larvae due to infection of B. thuringiensis, Lysinibacillus sphaericus, Klebsiella pneumonia and Pseudomonas paralactis (Astuti et al, 2018;Bravo et al, 2017;Devi et al, 2022) Andrejko & Mizerska-Dudka, 2012;Maciel-Vergara et al, 2018;Matsumoto, 2004;Ng et al, 2015;OztasGulmus & Gormez, 2020;Tarhriz et al, 2011;Vallet-Gely et al, 2008). These hydrolytic enzymes are known to aid in disrupting the cuticle, peritrophic matrix and hydrolyzing their lipoproteins, fats and other molecules present in the integument, causing its degradation (Lopes et al, 2021). and S. marcescens strain SEN (Aggarwal et al, 2015;Fite et al, 2020;Kaur et al, 2017;Mohan et al, 2008;Thakur et al, 2015).…”

Section: Discussionsupporting

confidence: 66%

“…catalases, proteases, chitinases, lipases, oxidases and phospholipases which have been reported to be produced by these bacterial isolates and other pathogenic bacterial strains such as Pseudomonas aeruginosa , Serratia and B. thuringiensis (Aggarwal et al, 2017; Andrejko & Mizerska‐Dudka, 2012; Maciel‐Vergara et al, 2018; Matsumoto, 2004; Ng et al, 2015; OztasGulmus & Gormez, 2020; Tarhriz et al, 2011; Vallet‐Gely et al, 2008). These hydrolytic enzymes are known to aid in disrupting the cuticle, peritrophic matrix and hydrolyzing their lipoproteins, fats and other molecules present in the integument, causing its degradation (Lopes et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Insecticidal, genotoxic and biochemical effects of Shewanella sp. (SS4) and Thauera sp. (M9) on Spodoptera litura

Sarkhandia

Devi

Sharma

et al. 2023

J Applied Entomology

View full text Add to dashboard Cite

Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae) is a polyphagous pest causing economic losses to a number of agricultural crops. The present study was conducted to explore the insecticidal potential of some soil bacteria against S. litura. Among the tested bacterial isolates, Shewanella sp. (SS4) and Thauera sp. (M9) caused higher larval mortality. Concentration–response bioassays indicated a dose‐dependent increase in larval mortality with maximum mortality of 80 and 76%, respectively, due to Shewanella sp. and Thauera sp. Both the bacteria negatively affected the growth and development, reduced adult emergence and induced morphological deformities in adults. Nutritional parameters of S. litura i.e. relative growth and consumption rate, efficiency of conversion of ingested and digested food as well as approximate digestibility of food decreased with increasing bacterial concentrations. Histopathological studies revealed the disruption of peritrophic membrane and midgut epithelium of larvae. Profiles of digestive enzymes i.e. α‐amylases, α, β‐glucosidases and galactosidases, lipases and proteases were also significantly decreased with bacterial treatment. Comet assay test indicated DNA damage in larval haemocytes due to bacterial infection. These results imply that both these bacterial isolates have the potential as biological control agents and may prove as environmentally friendly alternative for pest management techniques.

show abstract

Section: Discussionsupporting

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Insecticidal, genotoxic and biochemical effects of Shewanella sp. (SS4) and Thauera sp. (M9) on Spodoptera litura

Sarkhandia

Devi

Sharma

et al. 2023

J Applied Entomology

View full text Add to dashboard Cite

show abstract

“…Similarly, lipases can hydrolyze the lipoproteins, waxes and fats present in the insect integuments and compromise cuticle protection in insect pests and consequently expose the cuticular chitin and proteins to further degradation by chitinase and proteases, which causes insect mortality by desiccation due to the removal of the waterproof wax layer and exposure of internal organs to external aggressions [26]. For instance, lipases from B. subtilis strain Ehrenberg hydrolyzed the wax layer in the cuticle of Planococcus citri (Hemiptera: Pseudococcidae), causing lethal effect and significantly reducing female longevity, fecundity, and adult formation in the surviving citrus mealybugs [146].…”

Section: The Role Of Lipases From Bacillus Sp In Biological Controlmentioning

confidence: 99%

“…Interestingly, bacterial BCAs have been proved to be both non-toxic and can quickly drop back to natural (environmental) levels shortly after application because of the limited nutrient supply [22], and the biological buffering of the environment [23]. Thus, bacterial BCAs are increasingly becoming favorable alternatives to chemical pesticides [16,[24][25][26]. Consequently, various bacterial species including Bacillus sp., Pseudomonas sp., Streptomyces sp., Lysobacter sp., and Seratia sp.…”

Section: Introductionmentioning

confidence: 99%

The Prospect of Hydrolytic Enzymes from Bacillus Species in the Biological Control of Pests and Diseases in Forest and Fruit Tree Production

Ajuna,

Lim,

Moon

et al. 2023

IJMS

View full text Add to dashboard Cite

Plant diseases and insect pest damage cause tremendous losses in forestry and fruit tree production. Even though chemical pesticides have been effective in the control of plant diseases and insect pests for several decades, they are increasingly becoming undesirable due to their toxic residues that affect human life, animals, and the environment, as well as the growing challenge of pesticide resistance. In this study, we review the potential of hydrolytic enzymes from Bacillus species such as chitinases, β-1,3-glucanases, proteases, lipases, amylases, and cellulases in the biological control of phytopathogens and insect pests, which could be a more sustainable alternative to chemical pesticides. This study highlights the application potential of the hydrolytic enzymes from different Bacillus sp. as effective biocontrol alternatives against phytopathogens/insect pests through the degradation of cell wall/insect cuticles, which are mainly composed of structural polysaccharides like chitins, β-glucans, glycoproteins, and lipids. This study demonstrates the prospects for applying hydrolytic enzymes from Bacillus sp. as effective biopesticides in forest and fruit tree production, their mode of biocidal activity and dual antimicrobial/insecticidal potential, which indicates a great prospect for the simultaneous biocontrol of pests/diseases. Further research should focus on optimizing the production of hydrolytic enzymes, and the antimicrobial/insecticidal synergism of different Bacillus sp. which could facilitate the simultaneous biocontrol of pests and diseases in forest and fruit tree production.

show abstract