This article focuses on performing clinical, laboratory examination, isolation, and identification of Microsporum spp., general and biochemical analyzes of the blood of spontaneously infected animals and the successful treatment of Microsporum spp. infection in cats and dogs using miconazole/chlorhexidine combination and itraconazole. Skin scraping, hair samples and blood samples were collected from a total of 132 animals. Out of 132 examined dogs and cats, 43 animals were diagnosed with the disease, 28 of the sick animals were cats and 15 were dogs. Based on the results of RBCs (Red Blood Cell) Count, HGB (Hemoglobin), HCT (Hematocrit), MCH (Mean Corpuscular Hemoglobin) blood tests and MCHC (Mean Corpuscular Hemoglobin Concentration), low levels of healthy red blood cells, hemoglobin, and low results in MCHC blood tests were observed in the blood of all of infected dogs and cats, a high level of AST (Aspartate Aminotransferase) is also observed in some infected animals. Among the sick animals, there were also animals with normal AST (Aspartate Aminotransferase) levels. In our study, 29 infected cats and dogs were treated with itraconazole and one of two topical therapies including 2% chlorhexidine and 2% miconazole shampoo (VetWELL Micoseb Medicated Shampoo for Dogs & Cats - Medicated Dog Shampoo with Miconazole, Chlorhexidine & Aloe for Skin Infection Treatment of Skin Conditions 12oz). This study aimed to perform clinical characteristics, laboratory examination, isolation and identification of zoophilic dermatophytes due to Microsporum spp. In addition, to evaluate general and biochemical analyses of the blood of spontaneously infected pets and stray and shelter animals with Microsporum spp. in Baku and Ganja cities in Azerbaijan, and to focus the treatment includes systemic and topical therapy.