Microbial lipid production by cold‐adapted oleaginous yeast Yarrowia lipolytica B9 in non‐sterile whey medium

Abstract

Deproteinized whey was used as a substrate for the production of lipids by cold‐adapted yeast Yarrowia lipolytica B9 under non‐sterile culture conditions. Undesired microbial contamination in non‐sterile whey medium could be prevented when appropriate culture parameters (inoculum size of 3 mL/100 mL, initial pH of 5.5 and incubation temperature of 15 °C) were selected. In contrast to additional nitrogen (ammonium sulfate) and phosphorus (potassium dihydrogen phosphate) sources, additional carbon source (lactose) increased lipid accumulation. Under optimized culture conditions, biomass and lipid concentrations of the yeast were found as 7.4 g/L and 4.29 g/L, respectively. Lipid content was determined as 58% of total cell biomass. Fatty acids of the yeast were oleic acid (18:1), cis‐10‐heptadecenoic acid (C17:1), palmitoleic acid (16:1) and palmitic acid (16:0). The yeast was found to contain no polyunsaturated fatty acids. The content of C16 and C18 fatty acids was found to be 91.98% of total lipids. Monounsaturated fatty acids accounted for 80.54% of total lipids. Due to rich monounsaturated fatty acid composition, biomass of Y. lipolytica B9 may be used as feedstock for biodiesel production, especially operating in winter conditions. This is the first report on the use of cheese whey as a lipid production substrate for cold‐adapted microorganisms including Y. lipolytica yeast. Besides, lipid production potential of Y. lipolytica under non‐sterile culture conditions was investigated for the first time in this study. © 2015 Society of Chemical Industry and John Wiley & Sons, Ltd

Enhancement of lipid accumulation in oleaginous marine yeast; Candida sp. for biodiesel production and antimicrobial properties

“…The same finding was observed by Huang et al (2012). Another study by Taskin et al (2015) showed that the highest content of lipid and biomass of oleaginous yeast Yarrowia lipolytica B9 were (28%) and (4.38 gl -1 ) with an inoculum size of 3 %. Jiru et al (2017) reported that 10% v/v inoculum size was the optimum inoculum size for lipid accumulation by Rhodotorula kratochvilovae.…”

“…Similar feature was documented by Huang et al (2012) for oleaginous yeast; Trichosporon fermentans. Contrary, 15°C was favorable for oleaginous yeast Yarrowia lipolytica B9 (Taskin et al, 2015).…”

Evaluation of Waste Loquat Kernels as Substrate for Lipid Production by Rhodotorula glutinis SO28

“…That is to say the remaining sugar in the medium after the depletion of nitrogen source might be utilized for lipid synthesis. Because, investigators have reported that excessive carbon remains unutilized or is converted into storage polysaccharides in non-oleaginous species, while, it is preferentially channeled toward lipid synthesis in oleaginous species [14,25,32,34]. At the end of 132-hours cultivation period, the maximum lipid concentration and lipid content were determined as 7.82 g/L and 62 %, respectively.…”

“…At the end of 48-cultivation period, absorbance of yeast starter cultures was adjusted to 2.0 (at 600 nm) using sterile saline-water [25,26]. Then, 2 mL of yeast seed culture were used for inoculation of LKEB medium.…”

Acetate Production from Cafeteria Wastes and Corn Stover Using a Thermophilic Anaerobic Consortium: A Prelude Study for the Use of Acetate for the Production of Value-Added Products

“…This is important to achieve higher bioproduct yields and productivity and, therefore, can improve the overall economics of industrial microbial processes. An industrially relevant host organism is the oleaginous yeast-Yarrowia lipolytica-which has gained a lot of attention recently for the production of lipids using various pure substrate (e.g., glucose, fructose, lactose, sucrose, glycerol, and xylose) and raw feedstocks (e.g., olive oil mill waste, whey, waste cooking oil, and animal fats) [26,32]. Oleaginous yeasts have a distinctive ability to convert certain organic acids directly to acetyl-CoA-the central intermediate of lipid biosynthesis-by acetyl coenzyme A synthetase.…”