Microbial metabolism of quorum-sensing molecules acyl-homoserine lactones, γ-heptalactone and other lactones

Safari, Maryam; Amache, Rana; Esmaeilishirazifard, Elham; Keshavarz, Tajalli

doi:10.1007/s00253-014-5518-9

Cited by 35 publications

(19 citation statements)

References 119 publications

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“…Notably, the GcL substrate specificity extends to δ‐, γ‐, and ϵ‐lactones. Interestingly, some γ‐lactones are used as QS molecules in Streptomyces and Rhodococcus …”

Section: Resultsmentioning

confidence: 99%

The Structural Determinants Accounting for the Broad Substrate Specificity of the Quorum Quenching Lactonase GcL

et al. 2019

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Quorum quenching lactonases are enzymes capable of hydrolyzing lactones, including N‐acyl homoserine lactones (AHLs). AHLs are molecules known as signals in bacterial communication dubbed quorum sensing. Bacterial signal disruption by lactonases was previously reported to inhibit behavior regulated by quorum sensing, such as the expression of virulence factors and the formation of biofilms. Herein, we report the enzymatic and structural characterization of a novel lactonase representative from the metallo‐β‐lactamase superfamily, dubbed GcL. GcL is a broad spectrum and highly proficient lactonase, with kcat/KM values in the range of 104 to 106 m−1 s−1. Analysis of free GcL structures and in complex with AHL substrates of different acyl chain length, namely, C4‐AHL and 3‐oxo‐C12‐AHL, allowed their respective binding modes to be elucidated. Structures reveal three subsites in the binding crevice: 1) the small subsite where chemistry is performed on the lactone ring; 2) a hydrophobic ring that accommodates the amide group of AHLs and small acyl chains; and 3) the outer, hydrophilic subsite that extends to the protein surface. Unexpectedly, the absence of structural accommodation for long substrate acyl chains seems to relate to the broad substrate specificity of the enzyme.

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“…Notably, the GcL substrate specificity extends to δ‐, γ‐, and ϵ‐lactones. Interestingly, some γ‐lactones are used as QS molecules in Streptomyces and Rhodococcus …”

Section: Resultsmentioning

confidence: 99%

The Structural Determinants Accounting for the Broad Substrate Specificity of the Quorum Quenching Lactonase GcL

et al. 2019

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“…During a niche competition, bacteria present different strategies, one of which is the ability to interfere in the communication process of the other bacteria, system called quorum quenching (QQ) (Bassler, 2002). The bacterial communication, known as quorum sensing (QS), is used for many microorganisms to regulate the gene expression of them and enhance survival of the microorganism (Safari et al, 2014). In this work, we demonstrated by plates assays that Bacillus sp.…”

Section: Discussionmentioning

confidence: 99%

Molecular mechanisms involved in the bacterial talking and maize growth promotion

Almeida¹

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Molecular mechanisms involved in the bacterial talking and maize growth promotion With the increase of agricultural production, there is an improvement in the use of mineral fertilizers, which may cause different environmental problems, besides the soil salinization. A possible alternative for reducing the application of these products is the use of plant growth-promoting bacteria (PGPB), that can be used alone or in co-inoculation, resulting in an alternative environmentally and economically feasible. Better results can be obtained if the interaction among bacteria-bacteria and bacteria-plant be elucidated, and strategy developed to optimize these interactions. Thus, the plant growth-promoting Bacillus sp. RZ2MS9, previous described as a potential PGPB in maize and soybean, was GFP-tagged and monitored alone and co-inoculated with Azospirillum brasilense (Ab-v5::pWM1013) during maize colonization. The interaction of tagged strains in maize were monitored by fluorescent microscopy (FM) and quantitative PCR (qPCR), demonstrating an endophytic behavior of Bacillus sp. RZ2MS9. Although the non-detection of Ab-v5::pWM1013, the co-inoculation resulted in the best increase in root and shoot dried weight, root volume and in root diameter, showing that inoculation with more than one strain can be a good choice to development of bio-fertilizers. One important system to bacterial interaction is the quorum sensing (QS). The QS is an important cell-cell communication system that allows bacterial cells to recognize their own population and modulate their gene expression. This, system is also involved in the interspecific communication, including other bacterial species and plants. In the other hand, enzymes able to detect and degrade these molecules evolved, the called quorum quenching (QQ) system, that has been evolved in some bacteria as competitive advantage for niches colonization. The aiiA gene, was one of the first gene related with the QQ in Bacillus. The aiiA was found in Bacillus sp. RZ2MS9 genome. Through construction of a new QQ biosensor, Agrobacterium tumefaciens At11006, and validated by A. tumefaciens NTL4, the ability of RZ2MS9 to degrade QS molecules was confirmed. The knockout of aiiA gene was performed using the CRISPR-Cas9 system, confirming this gene function. By these results, the influence of QQ system of Bacillus sp. RZ2MS9 during maize colonization and RZ2MS9-A. brasilense-maize can be better investigated, opens the possibility to better understand the role of QQ system in the interaction among PGPB and plants.

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“…The cell-to-cell communication of microorganisms is known to be via exertion of certain chemical compounds (signal molecules) and is referred to as quorum sensing. QS phenomenon is widespread in microbial communities (Safari et al, 2014). It is known that bacteria utilize a quorum sensing system to coordinate gene expression by monitoring the concentration of molecules known as AI (Shao et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Effect of N-acyl homoserine lactones (AHLs) quorum sensing signal molecules on Enterococcus faecalis biofilm formation

Bensalem¹,

Djeribi²,

Baena³

2014

Afr. J. Microbiol. Res.

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The Gram-positive bacterium Enterococcus faecalis is an etiological agent of endodontic infections in oral biofilms. In the present study, we analyzed the effect of N-acyl homoserine lactones (AHLs) quorum sensing signal molecules on E. faecalis biofilm formation ability. Our results provide important effects of N-Hexanoyl-L-homoserine lactone (C6-HSL) and N-octanoyl-L-homoserine lactone (C8-HSL) molecules on E. faecalis biofilm formation. These AHLs molecules promoted the biofilm formation of E. faecalis.

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Microbial metabolism of quorum-sensing molecules acyl-homoserine lactones, γ-heptalactone and other lactones

Cited by 35 publications

References 119 publications

The Structural Determinants Accounting for the Broad Substrate Specificity of the Quorum Quenching Lactonase GcL

The Structural Determinants Accounting for the Broad Substrate Specificity of the Quorum Quenching Lactonase GcL

Molecular mechanisms involved in the bacterial talking and maize growth promotion

Effect of N-acyl homoserine lactones (AHLs) quorum sensing signal molecules on Enterococcus faecalis biofilm formation

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