Microbial Network and Soil Properties Are Changed in Bacterial Wilt-Susceptible Soil

Ma, Gaoqiang; Lin, Changchun; Qi, Gaofu

doi:10.1128/aem.00162-19

Cited by 72 publications

(69 citation statements)

References 62 publications

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“…It has been demonstrated that pH has a significant impact on bacterial abundance [32]. Some related reports predicted that soil acidification could negatively affect the bacterial community stability, leading to bacterial wilt [33]. However, we found that the pH was lower in SS samples than in CS; therefore, other soil chemical properties may contribute to the stability of the soil bacterial community or the susceptibility to RSSC.…”

Section: Discussioncontrasting

confidence: 60%

“…Purified amplicons were then pooled in equimolar concentrations and paired-end sequenced (2 × 300) using the Illumina MiSeq platform (Illumina, San Diego, CA, USA) according to the standard protocols of Shanghai Majorbio Bio-pharm Technology Co., Ltd. Raw sequences were filtrated using FASTX Toolkit 0.0.12 software to remove low quality reads with Q value < 20 and less than 35 bp [33].…”

Section: Methodsmentioning

confidence: 99%

“…Soil pH, OM content, AHN, RAP, and RAK were determined as previously reported [33]. RDA was conducted to calculate the bacterial diversity distribution correlation with the above soil chemical properties at the genus level.…”

Section: Correlation Between Soil Chemical Characteristics and Bactermentioning

confidence: 99%

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Comparison of bacterial communities in soil samples with and without tomato bacterial wilt caused by Ralstonia solanacearum species complex

Zhang

Zhou

et al. 2020

BMC Microbiol

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Background: Ralstonia solanacearum is one of the most notorious soil-borne phytopathogens. It causes a severe wilt disease with deadly effects on many economically important crops. The microbita of disease-suppressive soils are thought that they can contribute to the disease resistance of crop plants, thus, evaluation of the microbial community and their interaction characteristics between suppressive soil (SS) and conducive soil (CS) will help to understand resistance mechanism. To do this, the bacterial community structure, correlation analysis with soil chemical properties, interaction network of SS (nearly no disease in three years), and CS (suffered heavy bacterial wilt disease) were analyzed. Results: A higher bacterial community diversity index was found in SS, the relative abundance of Nocardioides, Gaiella and norank_f_Anaerolineaceae were significantly more than that of the CS. Moreover, the relative abundance of main genera Bacillus, norank_o_Gaiellales, Roseiflexus, and norank_o_Gemmatimonadaceae were significantly more than that of the CS. Redundancy analysis at the genus level indicated that the available phosphate played a key role in the bacterial community distribution, and its role was negatively correlated with soil pH, organic matter content, alkali-hydrolyzable nitrogen, and available potassium contents. Interaction network analysis further demonstrated that greater diversity at the genus level existed in the SS network and formed a stable network. Additionally, the species of Mycobacterium, Cyanobacteria, and Rhodobiaceae are the key components that sustain the network stability. Seven clusters of orthologous groups exhibited significant differences between SS and CS. Moreover, 55 bacterial strains with distinct antagonistic activities to R. solancearum were isolated and identified from the healthy tomato plant rhizosphere soil of the CS. Conclusions: Our findings indicate that the bacterial diversity and interaction network differed between the CS and SS samples, providing a good foundation in the study of bacterial wilt.

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Section: Discussioncontrasting

confidence: 60%

Section: Methodsmentioning

confidence: 99%

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Comparison of bacterial communities in soil samples with and without tomato bacterial wilt caused by Ralstonia solanacearum species complex

Zhang

Zhou

et al. 2020

BMC Microbiol

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show abstract

“…It has been demonstrated that pH has a significant impact on bacterial abundance [30]. Some related reports predicted that soil acidification could negatively affect the bacterial community stability, leading to bacterial wilt [31]. However, we found that the pH was lower in SS samples than in CS; therefore, other soil chemical properties may contribute to the stability of the soil bacterial community or the susceptibility to RSSC.…”

Section: Results Of Sobs Index Of the Otu Level Main Bacterial Commucontrasting

confidence: 60%

“…Soil pH, OM content, AHN, RAP, and RAK were determined as previously reported [31]. RDA was conducted to calculate the bacterial diversity distribution correlation with the above soil chemical properties at the genus level.…”

Section: Correlation Between Soil Chemical Characteristics and Bactermentioning

confidence: 99%

Comparison of Bacterial Communities in Soil Samples with and without Tomato Bacterial Wilt Caused by Ralstonia solanacearum Species Complex

Zhang

Zhou

et al. 2020

Preprint

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Background: Ralstonia solanacearum is one of the most notorious soil-borne phytopathogens. It causes a severe wilt disease with deadly effects on many economically important crops. The microbita of disease-suppressive soils are thought that they can contribute to the disease resistance of crop plants, thus, to conduct the microbial community and their interaction characteristics between suppressive soil (SS) and conducive soil (CS) will help to understand resistance mechanism. Here, the bacterial community structure, correlation analysis with soil chemical properties, interaction network of SS (nearly no disease in three years), and CS (suffered heavy bacterial wilt disease) were analyzed. Results: Compared with CS, a higher bacterial community diversity index was found in SS , and the relative abundance of main genera Bacillus , Gaiellales , Roseiflexus , Gemmatimonadaceae , Nocardioides , and Anaerolineacear reached significant levels. Redundancy analysis at the genus level indicated that the available phosphate played a key role in the bacterial community distribution, and its role was negatively correlated with soil pH, organic matter content, alkali-hydrolyzable nitrogen, and available potassium contents. Interaction network analysis further demonstrated that greater diversity at the genus level existed in the SS network and formed a stable network. Additionally, the species of Mycobacterium , Cyanobacteria , and Rhodobiaceae are the key components that sustain the network stability. Seven clusters of orthologous groups exhibited significant differences between SS and CS. Moreover, 55 bacterial strains with distinct antagonistic activities to R. solancearum were isolated and identified. Conclusions: Our findings indicate that the bacterial diversity and interaction network differed between the CS and SS samples, providing a good foundation in the study of bacterial wilt.

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Rhizospheric microbiota of suppressive soil protect plants against Fusarium solani infection

Li,

Yang,

Feng

et al. 2024

Pest Management Science

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BACKGROUNDFusarium infection has caused huge economic losses in many crops. The study aimed to compare the microbial community of suppressive and conducive soils and relate to the reduction of Fusarium wilt.RESULTSHigh‐throughput sequencing and microbial network analysis were used to investigate the differences in the rhizosphere microbiota of the suppressive and conducive soils and to identify the beneficial keystone taxa. Plant pathogens were enriched in the conducive soil. Potential plant‐beneficial microorganisms and antagonistic microorganisms were enriched in the suppressive soil. More positive interactions and keystone taxa existed in the suppressive soil network. 39 and 16 keystone taxa were respectively identified in the suppressive and conducive soil networks. 16 fungal strains and 168 bacterial strains were isolated from suppressive soil, some of which exhibited plant growth‐promotion traits. 39 bacterial strains and 10 fungal strains showed antagonistic activity against F. solani. Keystone taxa Bacillus and Trichoderma exhibited high antifungal activity. Lipopeptides produced by Bacillus sp. RB150 and chitinase from Trichoderma spp. inhibited the growth of F. solani. Microbial consortium I (Bacillus sp. RB150, Pseudomonas sp. RB70 and Trichoderma asperellum RF10) and II (Bacillus sp. RB196, Bacillus sp. RB150 and T. asperellum RF10) effectively controlled root rot disease, the spore number of F. solani was reduced by 94.2% and 83.3%.CONCLUSIONRhizospheric microbiota of suppressive soil protects plants against F. solani infection. Antagonistic microorganisms in suppressive soil inhibit pathogen growth and infection. Microbial consortia consisted of keystone taxa well control root rot disease. These findings help control Fusarium wilt.This article is protected by copyright. All rights reserved.

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Microbial Network and Soil Properties Are Changed in Bacterial Wilt-Susceptible Soil

Cited by 72 publications

References 62 publications

Comparison of bacterial communities in soil samples with and without tomato bacterial wilt caused by Ralstonia solanacearum species complex

Comparison of bacterial communities in soil samples with and without tomato bacterial wilt caused by Ralstonia solanacearum species complex

Comparison of Bacterial Communities in Soil Samples with and without Tomato Bacterial Wilt Caused by Ralstonia solanacearum Species Complex

Rhizospheric microbiota of suppressive soil protect plants against Fusarium solani infection

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