Microbial pathogens from goat mastitis and phage-typing of Staphylococcus aureus Isolates

Al-Graibawi, Mawlood Abbas Ali; Sharma, VK; Al-Shammari, Abdul Jabbar N.

doi:10.1016/0147-9571(86)90071-8

Cited by 9 publications

(5 citation statements)

References 3 publications

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“…Streptococci spp. are considered as the most common cause of the clinical as well as SCM in goats after Staphylococci [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Prevalence and bacterial etiology of subclinical mastitis in goats reared in organized farms

Mishra¹,

Sharma²,

Singh³

et al. 2018

Vet World

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Aim:Assessment of the status of subclinical mastitis (SCM) in Jamunapari and Barbari goats in Indian organized farms, the involvement of bacterial pathogens and their sensitivity to antibiotics.Materials and Methods:A total of 181 composite milk samples were aseptically collected from the apparently healthy Barbari (n=95) and Jamunapari (n=86) goats. The California mastitis test (CMT) and somatic cell count (SCC) were used to diagnose SCM. The milk samples with CMT scores of 0 and +1 were considered as negative, while the samples with the score of +2 or +3 were taken as positive, and further, the positive samples were used for the bacteriological examination. An antibiotic sensitivity test was performed by disk diffusion method using seven commercially available antibiotic discs.Results:All the samples having CMT score of +2 or +3 demonstrated SCC more than 1 million. Overall, the prevalence of SCM in the goats was assessed as 19.89% (36/181). The prevalence of SCM in Barbari and Jamunapari goats was found as 24.21% (23/95) and 15.12% (13/86), respectively. Out of 11 isolates of Staphylococci, 9 isolates were identified as coagulase-negative Staphylococci (CNS), whereas 2 isolates were found as Staphylococcus aureus. The identified bacterial isolates (n=30) did not show antibiotic resistance.Conclusion:The current investigation showed the considerable prevalence of SCM among Jamunapari and Barbari goats which may have a negative impact on quantity and quality of the milk. CNS was found as the most prevalent cause of SCM in the goats. Negligible antibiotic resistance was found among the identified udder pathogens.

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“…Streptococci spp. are considered as the most common cause of the clinical as well as SCM in goats after Staphylococci [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Prevalence and bacterial etiology of subclinical mastitis in goats reared in organized farms

Mishra¹,

Sharma²,

Singh³

et al. 2018

Vet World

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“…S. aureus is the most frequently isolated pathogen known to be involved in mammary infections of goats (21–25). Consequently, to be a robust method, any protocol for identifying mycoplasma infection of milk must be capable of distinguishing between Mycoplasma spp.…”

Section: Discussionmentioning

confidence: 99%

Detection of mycoplasmas in goat milk by flow cytometry

et al. 2007

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The detection of mycoplasma in milk can be performed by either culture techniques or polymerase chain reaction (PCR) based methods. Although PCR can reduce the average diagnostic time to 5 h in comparison with the several days for the isolation of the agent, there is still a need to develop methods, which could give earlier results. For this purpose, we tested the ability of flow cytometry (FC) to detect mycoplasmas in milk samples. Milk samples inoculated with four different mycoplasmas, Mycoplasma agalactiae, Mycoplasma putrefaciens, Mycoplasma capricolum subsp. Capricolum, or Mycoplasma mycoides subsp. mycoides large-colony type, known to cause contagious agalactia in goats, were stained with the DNA stain SYBR Green I and analyzed by FC. Three goat milk samples, from which mycoplasmas have been isolated in broth medium were also analyzed. All mycoplasmas were easily distinguished from debris of milk samples, but it was not possible to distinguish between the different mycoplasma species. In our conditions, the detection limit of the technique was of the order of 10 3 -10 4 cells ml 21 . Furthermore, mycoplasmas were also distinguished from Staphylococcus aureus. FC together with SYBR Green I was able to distinguish between mycoplasma cells and debris present in milk samples and gave results in 20-30 min. This is an important first step in developing a robust, routine flow cytometric method for the detection of mycoplasmas in milk samples. '

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“…Pasteurella spp. Czynnikiem etiologicznym mastitis jest głównie Pasteurella haemolytica, rzadziej P. multocida (2). Jest to bakteria Gram-ujemna, która jest komensalnie pospolitym organizmem migdałków i nosogardzieli zdrowych kóz (1).…”

Section: Zapalenia Wymienia Wywoływane Przezunclassified

Mastitis in goats – a still current problem

WOCHNIK,

SZCZUBIAŁ,

DĄBROWSKI

2024

Medycyna Weterynaryjna

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Even though goats are one of the first animal species domesticated by humans, udder inflammation in these female farm animals still poses major diagnostic and therapeutic challenges for practicing veterinarians. Mastitis in goats, similarly to cattle, causes large economic losses, including breeding losses and losses in the dairy industry. For this reason, the most important information regarding the diagnosis and treatment of mastitis in goats has been collected in this study, based on the latest data from the literature. Attention was drawn to the fact that in many forms of mastitis, to achieve the desired therapeutic effect it is crucial to undertake quick and targeted therapy. The study also emphasized the need to develop new laboratory diagnostic techniques aimed at determining indicators of the inflammatory reaction not only in milk and blood serum but also in saliva collected from females suffering from mastitis. This enables a quick and effective diagnosis of the disease and an effective treatment method.

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Microbial pathogens from goat mastitis and phage-typing of Staphylococcus aureus Isolates

Cited by 9 publications

References 3 publications

Prevalence and bacterial etiology of subclinical mastitis in goats reared in organized farms

Prevalence and bacterial etiology of subclinical mastitis in goats reared in organized farms

Detection of mycoplasmas in goat milk by flow cytometry

Mastitis in goats – a still current problem

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