Microbial population dynamics during startup of a full-scale anaerobic digester treating industrial food waste in Kyoto eco-energy project

Microbial Community Succession during Lactate Amendment and Electron Acceptor Limitation Reveals a Predominance of Metal-Reducing Pelosinus spp

“…and the hydrogenotrophic Methanobacterium spp., suggesting multiple routes of carbon mineralization from fermentation by-products in situ. However, there was no obvious correlation between temporal species abundances and volatile fatty acid or gas concentrations, similar to findings from studies of anaerobic sludge and food waste reactors (25,56). However, further investigation into archaeal community revealed that several OTUs, while not initially detectable in the original or early temporal samples, were able to maintain low-level populations despite numerous reactor turnovers and became robust in later time periods.…”

Microbial community dynamics in mesophilic anaerobic co-digestion of mixed waste

“…Thus, seeding the waste appears to confer community stability during the earlier stages of waste treatment (stages 1 and 2) prior to entering the AD digester at stage 3. Adding an established microbial community or 'seed' prior to AD is common practice within the industry and has been shown to reduce the lag phase and improve bioconversion efficiency and system stability (Kobayashi et al, 2009;Ike et al, 2010). This study reinforces the importance of seeding the waste with an established community as part of the AD process.…”

“…Lactobacillus and Acinetobacter were found at stage 1 and 3 and one of the Psychrobacter sequences was only recovered from stages 1 and 2. The retrieved sequences were closely related to those of uncultured bacteria reported previously in MSW anaerobic digesters (Leven et al, 2007;Cardinali-Rezende et al, 2009;Ike et al, 2010;Trzcinski et al, 2010). Interestingly, Bacteroidetes which have been shown in other studies to be major microbial components of anaerobic reactors (Leven et al, 2007;Trzcinski et al, 2010) were not recovered from our samples.…”

“…The different trophic groups recovered during stages 1 and 2 suggest that the digestion of mixed wastes (MSW, FVW and NSW) mainly supports acetate, lactate-and butyrate-producing bacteria. This is common for the AD of such waste streams (Ike et al, 2010). The volatile fatty acids (VFAs) were degraded further by the methanogens in the main digester (Stage 3, sludge waste).…”

Influence of Sewage Sludge and Leachate on Biochemical Methane Potential of Waste Biomass

“…Other studies reported that microbial load in leachate consist of 16S rRNA genes which enhances the rate of biodegradation of organic waste [32,33].…”