Microbial population in a thermophilic packed-bed reactor for methanogenesis from volatile fatty acids

Ueno, Yoshiyuki; Tatara, Masahiro

doi:10.1016/j.enzmictec.2008.04.007

Cited by 16 publications

(8 citation statements)

References 32 publications

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“…M. thermoautotrophicum (formerly M e t h a n o b a c t e r i u m t h e r m o a u t o t ro p h i c u m ) is a lithoautotrophic and thermophilic archaeon (Zeikus and Wolee 1972), which was first isolated from sewage sludge in 1971. It has been frequently detected in anaerobic digestion processes (Luo and Angelidaki 2013), including some thermophilic digestion studies (Tada et al 2006;Ueno and Tatara 2008). Overall, the enrichment of Methanobacterium and Methanothermobacter species in thermophilic conditions suggested their importance in producing methane in TAD.…”

Section: Important Archaeal Genera and Speciesmentioning

confidence: 97%

From mesophilic to thermophilic digestion: the transitions of anaerobic bacterial, archaeal, and fungal community structures in sludge and manure samples

Sun

Louie

et al. 2015

Appl Microbiol Biotechnol

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The shift of microbial communities during a transition from mesophilic anaerobic digestion (MAD) to thermophilic anaerobic digestion (TAD) was characterized in two treatments. One treatment was inoculated with sludge and the other was inoculated with manure. In this study, methane was produced both in MAD and TAD, but TAD has slightly more methane produced than MAD. A broad phylogenetic spectrum of bacterial, archaeal, and fungal taxa at thermophilic conditions was detected. Coprothermobacter, Bacillus, Haloplasma, Clostridiisalibacter, Methanobacterium, Methanothermobacter, Saccharomycetales, Candida, Alternaria, Cladosporium, and Penicillium were found almost exclusively in TAD, suggesting their adaptation to thermophilic conditions and ecological roles in digesting the organic compounds. The characterization of the lesser-known fungal community revealed that fungi probably constituted an important portion of the overall community within TAD and contributed to this process by degrading complex organic compounds. The shift of the microbial communities between MAD and TAD implied that temperature drastically affected the microbial diversity in anaerobic digestion. In addition, the difference in microbial communities between sludge and manure indicated that different source of inoculum also affected the microbial diversity and community.

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Section: Important Archaeal Genera and Speciesmentioning

confidence: 97%

From mesophilic to thermophilic digestion: the transitions of anaerobic bacterial, archaeal, and fungal community structures in sludge and manure samples

Sun

Louie

et al. 2015

Appl Microbiol Biotechnol

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“…A number of as-yet-uncultivated M. crinale-related phylotypes have been described in geographically distant high-temperature oil reservoirs such as oil fields on the north slope of Alaska (11,15), the Yabase oil field in Japan (33), the Dagang oil field (40) and the Qinghuang unit in the People's Republic of China (26), high-temperature natural gas fields (38), and oil-polluted saline soil (63) (see Table S1 in the supplemental material). More interestingly, the oc- (57), and swine manure, sewage sludge, and a hot-rot compost suspension (23).…”

Section: Morphology Strain Tm2mentioning

confidence: 99%

Isolation and Characterization of Methanothermobacter crinale sp. nov., a Novel Hydrogenotrophic Methanogen from the Shengli Oil Field

Cheng

Dai

et al. 2011

Appl Environ Microbiol

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Syntrophic acetate oxidation coupled with hydrogenotrophic methanogenesis is an alternative methanogenic pathway in certain thermophilic anaerobic environments such as high-temperature oil reservoirs and thermophilic biogas reactors. In these environments, the dominant thermophilic methanogens were generally related to uncultured organisms of the genus Methanothermobacter. Here we isolated two representative strains, Tm2T and HMD, from the oil sands and oil production water in the Shengli oil field in the People's Republic of China. The type strain, Tm2T , was nonmotile and stained Gram positive. The cells were straight to slightly curved rods (0.3 m in width and 2.2 to 5.9 m in length), but some of them possessed a coccal shape connecting with the rods at the ends. Strain Tm2 T grew with H 2 -CO 2 , but acetate is required. Optimum growth of strain Tm2T occurred in the presence of 0.025 g/liter NaCl at pH 6.9 and a temperature of 65°C. The G؉C content of the genomic DNA was 40.1 mol% ؎ 1.3 mol% (by the thermal denaturation method) or 41.1 mol% (by high-performance liquid chromatography). Analysis of the 16S rRNA gene sequence indicated that Tm2 Methanogenesis is the terminal process of organic compound degradation and plays a major role in the global carbon cycle, occurring in a variety of natural and artificial environments, such as the gastrointestinal tracts of animals, rice paddy soils, deep subsurface marine or freshwater sediments, and anaerobic bioreactors (14, 28). The most important precursors for methane production during anaerobic digestion of organic matter are acetate and H 2 -CO 2 , which are converted into methane by aceticlastic and hydrogenotrophic methanogens (28), respectively. However, an alternative pathway for acetate is syntrophic acetate oxidation followed by hydrogenotrophic methanogenesis (65). Several bacteria responsible for acetate oxidation have been isolated and characterized (17,25,48,60), and a series of reports revealed that this process is present in high-temperature oil reservoirs (15,33,40), thermophilic or mesophilic biogas reactors (16,21,23,43,49), lake sediments (41), and rice paddy soils (27). Interestingly, it is proposed that syntrophic acetate oxidation coupled to hydrogenotrophic methanogenesis is the main methanogenic pathway in hightemperature petroleum reservoirs (33) and probably involved in the thermophilic methanogenic degradation of hydrocarbon (15). Cloning of rRNA genes from these environments indicates that the dominant thermophilic hydrogenotrophic methanogens were found to be affiliated with the genus Methanothermobacter. However, to our knowledge, pure isolates have not been described. Here, we report the isolation and characterization of two strains, Tm2T and HMD, belonging to a novel phylotype of Methanothermobacter. MATERIALS AND METHODSSamples and media. The production water of oil reservoirs was collected from the Shengli oil field in 2007 and maintained in our lab at a temperature of 4°C. The oil reservoir is located 1,680 to 1,800 m below the sea...

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“…A total of 78 Bacteria clones were classified into 22 OTUs in 9 divisions, including Firmicutes, Proteobacteria, Chloroflexi, Thermotogae, Bacteroidetes/Chlorobi, Spirochaetes, Acidobacteria and 2 candidate divisions (Liang et al 2009). Ueno and Tatara (2008) investigated microbial community in thermophilic methane production from organic acids in a packed-bed reactor using 16S rDNA clone analysis showed that microbial populations changed with hydraulic retention time (HRT), and Thermoanaerobacteriaceae was mainly present in the biofilm with hydrogenotrophic methanogens whereas clones that are affiliated to Clostridiaceae were mainly found in the fermentation broth. It was also found that a certain amount of unknown or unidentified organisms were present in the microflora.…”

Section: Identification Of New Speciesmentioning

confidence: 99%

Application of Molecular Methods for Anaerobic Technology

Zhang¹

2010

Environmental Anaerobic Technology

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Anaerobic technology has been applied to sludge digestion for over 50 years and to wastewater treatment for nearly 30 years. This chapter reviews the nucleic acids-based molecular methods for the analysis of microbial communities, and their applications for the microbial communities in anaerobic reactors. At first, nucleic acid extraction and selection of nucleic acid biomarkers are discussed with emphasis on special aspects related to anaerobic sludge samples. Second, the common nucleic acids-based methods are introduced, including cloning and sequencing for characterization, quantitative real-time polymerase chain (qRT-PCR) for quantification, fluorescence in situ hybridization (FISH) for visualization, denaturing gradient gel electrophoresis (DGGE) and terminal restriction fragment length polymorphism (T-RFLP) for screening/typing/monitoring, and stable isotope probing (SIP) and microautoradiography-FISH to link the microbial identity with the specific functions. Finally, applications of the aforementioned molecular methods in anaerobic technology are summarized, focusing on identification of new species in the anaerobic process, characterization of microbial compositions in the anaerobic reactors, visualization of layered structure of anaerobic granular sludge, etc.

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Microbial population in a thermophilic packed-bed reactor for methanogenesis from volatile fatty acids

Cited by 16 publications

References 32 publications

From mesophilic to thermophilic digestion: the transitions of anaerobic bacterial, archaeal, and fungal community structures in sludge and manure samples

From mesophilic to thermophilic digestion: the transitions of anaerobic bacterial, archaeal, and fungal community structures in sludge and manure samples

Isolation and Characterization of Methanothermobacter crinale sp. nov., a Novel Hydrogenotrophic Methanogen from the Shengli Oil Field

Application of Molecular Methods for Anaerobic Technology

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