Microbial production of 2,3-butanediol by a surfactant (serrawettin)-deficient mutant of Serratia marcescens H30

Abstract

Serrawettin W1 produced by Serratia marcescens is a surface-active exolipid resulting in a lot foam formation during the 2,3-butanediol (2,3-BD) fermentation process. In order to avoid excessive addition of antifoam agent and microbial contamination, S. marcescens mutants deficient in serrawettin W1 formation were successfully constructed through insertional inactivation of the swrW gene coding for serrawettin W1 synthase. The shake flask and batch experiments suggested that disruption of the swrW gene led to significant reduction of the foam formation and improved 2,3-BD production a little. Ultimately, fed-batch culturing of the mutant afforded a maximum 2,3-BD concentration of 152 g l(-1) with a productivity of 2.67 g l(-1) h(-1) and a yield of 92.6% at 57 h.

Hydrodynamics of a self-actuated bacterial carpet using microscale particle image velocimetry

“…This can be attributed to the dissipation of flow due to a thin lubrication layer composed of secreted surface-active cyclodepsipeptides, e.g., Serrawettin W1, produced by S. marcescens, which act as wetting agents. 37 Comparing with the average velocity of the moving MBR, which is 5.63 lm/s, the ensemble average velocity of the flow field was higher, by a factor of 2, which supports the hypothesis that the self-actuation motion was generated by strong flow fields interacting with the boundary at the bio surfactant lubrication layer. The fluctuation of the ensemble average velocities in both experiments, shown in Fig.…”

Transformation of Biomass into Commodity Chemicals Using Enzymes or Cells

“…182 The yield on sucrose was 0.49 g/g, whereas the theoretical yield on sucrose is 2 mol/mol, corresponding to 0.53 g/g. The productivity was 2.7 g/(L h).…”

Genome Sequence of meso -2,3-Butanediol-Producing Strain Serratia marcescens ATCC 14041

“…In addition, meso -2,3-BD can also be used to produce renewable polyesters, such as semi-aromatic polyesters for coating applications (4, 5). Microbial production of 2,3-BD has a long history, and various bacteria, such as Serratia marcescens , Bacillus licheniformis , and Klebsiella pneumoniae , were used to produce 2,3-BD (2, 6–9). However, among the native 2,3-BD producers, only S. marcescens was reported to be able to produce meso -2,3-BD as the major product (2).…”