Microbial production of branched chain amino acids: Advances and perspectives

Hao, Yanan; Pan, Xuewei; You, Jiajia; Li, Guomin; Xu, Meijuan; Rao, Zhiming

doi:10.1016/j.biortech.2024.130502

Cited by 4 publications

(1 citation statement)

References 108 publications

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“…The integration of rational metabolic engineering, coupled with the application of novel synthetic biology tools, offers promising solutions to this issue. Dynamic regulation remains a viable metabolic modification strategy for significantly enhancing the biosynthesis of POP-AAs [ 231 ].…”

Section: Conclusion and Perspectivementioning

confidence: 99%

Recent Advances in Metabolic Engineering for the Biosynthesis of Phosphoenol Pyruvate–Oxaloacetate–Pyruvate-Derived Amino Acids

Yin,

Zhou,

Ding

et al. 2024

Molecules

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The phosphoenol pyruvate–oxaloacetate–pyruvate-derived amino acids (POP-AAs) comprise native intermediates in cellular metabolism, within which the phosphoenol pyruvate–oxaloacetate–pyruvate (POP) node is the switch point among the major metabolic pathways existing in most living organisms. POP-AAs have widespread applications in the nutrition, food, and pharmaceutical industries. These amino acids have been predominantly produced in Escherichia coli and Corynebacterium glutamicum through microbial fermentation. With the rapid increase in market requirements, along with the global food shortage situation, the industrial production capacity of these two bacteria has encountered two bottlenecks: low product conversion efficiency and high cost of raw materials. Aiming to push forward the update and upgrade of engineered strains with higher yield and productivity, this paper presents a comprehensive summarization of the fundamental strategy of metabolic engineering techniques around phosphoenol pyruvate–oxaloacetate–pyruvate node for POP-AA production, including L-tryptophan, L-tyrosine, L-phenylalanine, L-valine, L-lysine, L-threonine, and L-isoleucine. Novel heterologous routes and regulation methods regarding the carbon flux redistribution in the POP node and the formation of amino acids should be taken into consideration to improve POP-AA production to approach maximum theoretical values. Furthermore, an outlook for future strategies of low-cost feedstock and energy utilization for developing amino acid overproducers is proposed.

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Section: Conclusion and Perspectivementioning

confidence: 99%

Recent Advances in Metabolic Engineering for the Biosynthesis of Phosphoenol Pyruvate–Oxaloacetate–Pyruvate-Derived Amino Acids

Yin,

Zhou,

Ding

et al. 2024

Molecules

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Changes in metabolite profiles in solid fermentation of glutamate waste treatment solution by Aspergillus niger and Candida tropicalis

He,

Zhang,

Liu

et al. 2024

Journal of Applied Microbiology

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Aims Glutamate wastewater poses a great environmental challenge to the monosodium glutamate production industry. However, its treatment solution is rich in crude protein, which has the potential to be developed as a new protein source for animal feed. Methods and results Given that the fermentation process generates functionally different metabolites, this study innovatively utilized two strains of feed microorganisms, Aspergillus niger and Candida tropicalis, to perform solid-state fermentation of glutamate wastewater treatment solution. The aim was to investigate and analyze the metabolite profiles during fermentation. The significant differences in metabolite profiles between the samples were determined using correlation analysis, principal component analysis (PCA), orthogonal partial least squares discriminant analysis (OPLS-DA), variable importance in projection (VIP) analysis, Kyoto Encyclopaedia of Genomes (KEGG), and Human Metabolome Data Bank (HMDB) analysis. These variations were mainly manifested in essential feed components, such as amino acids, peptides, and their analogues. These included Ile-Pro-Asn, Pro-Gly-Val, alanylvaline, histidylisoleucine, Lys-Leu-Tyr, Ile-Arg, glycyl-leucine, leucyl-lysine, N-palmitoyl histidine, alanylisoleucine, L-glutamate, N-methylisoleucine, lsoleucylproline, DL-m-tyrosine, lsoleucyl-threonine, phenylalanine amide, carboxyethyllysine, N6-acetyl-L-lysine, citrulline, N-alpha-acetyl-L-lysine, N(6)-methyllysine, and L-aspartate-semialdehyde. Conclusions This study investigates the metabolite profiles of glutamate wastewater treatment solutions after co-fermentation with Aspergillus niger and Candida tropicalis using solid-state fermentation. These findings provide a new strategy for efficiently utilizing glutamate wastewater treatment solutions.

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Production of α-ketoisovalerate with whey powder by systemic metabolic engineering of Klebsiella oxytoca

Sun,

Wang,

Tan

et al. 2024

Microb Cell Fact

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Microbial production of branched chain amino acids: Advances and perspectives

Cited by 4 publications

References 108 publications

Recent Advances in Metabolic Engineering for the Biosynthesis of Phosphoenol Pyruvate–Oxaloacetate–Pyruvate-Derived Amino Acids

Recent Advances in Metabolic Engineering for the Biosynthesis of Phosphoenol Pyruvate–Oxaloacetate–Pyruvate-Derived Amino Acids

Changes in metabolite profiles in solid fermentation of glutamate waste treatment solution by Aspergillus niger and Candida tropicalis

Production of α-ketoisovalerate with whey powder by systemic metabolic engineering of Klebsiella oxytoca

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