Microbial Production of Flavors and Fragrances

Mikš-Krajnik, Marta; Zoglowek, Marta; Buron-Moles, G.; Förster, Jochen

doi:10.1007/978-3-319-50436-0_375

Cited by 3 publications

(3 citation statements)

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“…Particularly, isoamyl acetate has a characteristic banana aroma and is a particularly important ester utilized in developing products from the food, cosmetic, and pharmaceutical industries (Abbas, 2006). Production of isoamyl acetate occurs mainly by chemical synthesis, but there is an increasing importance of biotechnological processes (Longo and Sanromán, 2006): enzymatic synthesis Mhetras et al, 2010;Osorio-Viana et al, 2014;Vakili et al, 2020;Paulino et al, 2021) and fermentation (Yilmaztekin et al, 2009;Rentería-Martínez et al, 2016;Sánchez-Castañeda et al, 2018). Microbial synthesis of isoamyl acetate is performed intracellularly by the esterification of the isoamyl alcohol and acetyl-CoA in a reaction mediated by the enzymes alcohol acetyltransferases I and II (Fuji et al, 1994;Yoshimoto et al, 1998;Procopio et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Enhancing isoamyl acetate biosynthesis by Pichia fermentans

Rentería-Martínez¹,

Páez-Lerma²,

Rojas‐Contreras³

et al. 2021

RMIQ

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Section: Introductionmentioning

confidence: 99%

Enhancing isoamyl acetate biosynthesis by Pichia fermentans

Rentería-Martínez¹,

Páez-Lerma²,

Rojas‐Contreras³

et al. 2021

RMIQ

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“…However, obtaining limonene by plant extraction is vulnerable to climatic and seasonal uncertainties, feedstock variability, and agricultural acreage . The chemical synthesis route of limonene production is unfavorable due to the high energy consumption, low efficiency, and environmental damage it causes . Therefore, there is a demand for alternative routes to produce limonene industrially.…”

mentioning

confidence: 99%

“…8 The chemical synthesis route of limonene production is unfavorable due to the high energy consumption, low efficiency, and environmental damage it causes. 9 Therefore, there is a demand for alternative routes to produce limonene industrially. In the past decade, the rapid development of metabolic engineering and synthetic biology in microbes has provided new and promising alternative avenues for producing a series of value-added products in a sustainable and environmentally friendly way.…”

mentioning

confidence: 99%

Simultaneous Improvement of Limonene Production and Tolerance in Yarrowia lipolytica through Tolerance Engineering and Evolutionary Engineering

Zhu

Ling

et al. 2021

ACS Synth. Biol.

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Limonene is an important plant natural product widely used in food and cosmetics production as well as in the pharmaceutical and chemical industries. However, low efficiency of plant extraction and high energy consumption in chemical synthesis limit the sustainability of industrial limonene production. Recently, the advancement of metabolic engineering and synthetic biology has facilitated the engineering of microbes into microbial cell factories for producing limonene. However, the deleterious effects on cellular activity by the toxicity of limonene is the major obstacle in achieving high-titer production of limonene in engineered microbes. In this study, by using transcriptomics, we identified 82 genes from the nonconventional yeast Yarrowia lipolytica that were up-regulated when exposed to limonene. When overexpressed, 8 of the gene candidates improved tolerance of this yeast to exogenously added limonene. To determine whether overexpression of these genes could also improve limonene production, we individually coexpressed the tolerance-enhancing genes with a limonene synthase gene. Indeed, expression of 5 of the 8 candidate genes enhanced limonene production in Y. lipolytica. Particularly, overexpressing YALI0F19492p led to an 8-fold improvement in product titer. Furthermore, through short-term adaptive laboratory evolution strategy, in combination with morphological and cytoplasmic membrane integrity analysis, we shed light on the underlying mechanism of limonene cytotoxicity to Y. lipolytica. This study demonstrated an effective strategy for improving limonene tolerance of Y. lipolytica and limonene titer in the host strain through the combinatorial use of tolerance engineering and evolutionary engineering.

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