Microbial production of medium chain fructooligosaccharides by recombinant yeast secreting bacterial inulosucrase

Ko, Hyunjun; Bae, Jung Hoon; Sung, Bong Hyun; Kim, Mi Jin; Park, Hyun-Ju; Sohn, Jae Hak

doi:10.1016/j.enzmictec.2019.109364

Cited by 15 publications

(6 citation statements)

References 20 publications

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“…Moreover, the necessity of a high acetonitrile content in the mobile phase results in a poor solubility of the higher-molecular substances (e.g., polysaccharides) continuously precipitating with the danger of capillary or column clogging within the HPLC system. Hydrogen H + Application example: Fermentation broth from FOS production [13].…”

Section: Discussionmentioning

confidence: 99%

Quantification of Fructans in Bioethanol Stillage Based On a Simplified Analytical Method

Zimmermann¹,

Kaltschmitt²

2021

Preprint

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Bioethanol stillage, the main by-product of industrial bioethanol production, is a potential substrate for fructans. However, the determination and quantification of fructans in such complex sample matrices is still a challenge for the corresponding analytics to be overcome in order to allow for the identification and utilisation of such unused fructan sources. Especially a possible utilisation or rather the corresponding process development requires appropriate analytics first. Thus, this paper aims to illuminate the basics of fructan quantification in stillage and the corresponding challenges particularly arising with widely used HPLC-RID systems. On this basis, a new approach for fructan quantification is presented based on such HPLC-RID systems allowing for a reliable and especially simple fructan determination in bioethanol stillage for comparably high sample throughput. The developed method performs fructan quantification by determination of fructose and glucose equivalents after a targeted acidic hydrolysis adapted to the respective sample matrix. By means of two different stationary phases, the problem of limited resolution in case of HPLC-RID is overcome and thus measurement errors are reduced. The approach towards the adapted analytical method can be transferred easily to comparable complex sample matrices.

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Section: Discussionmentioning

confidence: 99%

Quantification of Fructans in Bioethanol Stillage Based On a Simplified Analytical Method

Zimmermann¹,

Kaltschmitt²

2021

Preprint

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“…Ko et al . reported the first secretory production of bacterial ISRase from Lactobacillus reuteri ( INU1 )[ 64 ]. To achieve secretory production of ISRase in yeast, truncated INU1 variants were constructed because the carboxy-terminus of INU1 contains a cell wall-anchoring domain.…”

Section: Microbial Production Of Fructan By Yeast Cell Factoriesmentioning

confidence: 99%

“…The highest FOS titer was 152.6 g/l with 400 g/l sucrose, and the highest conversion efficiency was 42.7% with 300 g/l sucrose (128 g/l FOS). By ion chromatography analysis, the produced FOS was identified as mcFOS ranging from DP to 2–20; suggesting one-step production of mcFOS instead of a two-step process, involving extraction of inulin from plants and subsequent enzymatic treatment [ 64 ].…”

Section: Microbial Production Of Fructan By Yeast Cell Factoriesmentioning

confidence: 99%

Fructan Biosynthesis by Yeast Cell Factories

Sung

Kim

et al. 2022

J. Microbiol. Biotechnol.

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Fructan is a polysaccharide composed of fructose and can be classified into several types, such as inulin, levan, and fructo-oligosaccharides, based on their linkage patterns and degree of polymerization. Owing to its structural and functional diversity, fructan has been used in various fields including prebiotics, foods and beverages, cosmetics, and pharmaceutical applications. With increasing interest in fructans, efficient and straightforward production methods have been explored. Since the 1990s, yeast cells have been employed as producers of recombinant enzymes for enzymatic conversion of fructans including fructosyltransferases derived from various microbes and plants. More recently, yeast cell factories are highlighted as efficient workhorses for fructan production by direct fermentation. In this review, recent advances and strategies for fructan biosynthesis by yeast cell factories are discussed.

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“…On the other hand, Cys242 is not related with protein folding but affects the final product type (Li et al 2008;Kaplan-Turkoz and Bakar 2017). Although the heterologous production of FOS by different organisms has proven to be a successful strategy to optimize and change the FOS composition (Zhang et al 2017;Hernández et al 2018;Ko et al 2019;Rodrigo-Frutos et al 2019), there are no reports regarding the heterologous production of FOS by Z. mobilis.…”

Section: Introductionmentioning

confidence: 99%

Tailoring fructooligosaccharides composition with engineered Zymomonas mobilis ZM4

Braga

Gomes

Rainha

et al. 2022

Appl Microbiol Biotechnol

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Zymomonas mobilis ZM4 is an attractive host for the development of microbial cell factories to synthesize high-value compounds, including prebiotics. In this study, a straightforward process to produce fructooligosaccharides (FOS) from sucrose was established. To control the relative FOS composition, recombinant Z. mobilis strains secreting a native levansucrase (encoded by sacB) or a mutated β-fructofuranosidase (Ffase-Leu196) from Schwanniomyces occidentalis were constructed. Both strains were able to produce a FOS mixture with high concentration of 6-kestose. The best results were obtained with Z. mobilis ZM4 pB1-sacB that was able to produce 73.4 ± 1.6 g L −1 of FOS, with a productivity of 1.53 ± 0.03 g L −1 h −1 and a yield of 0.31 ± 0.03 g FOS g sucrose −1. This is the first report on the FOS production using a mutant Z. mobilis ZM4 strain in a one-step process. Key points• Zymomonas mobilis was engineered to produce FOS in a one-step fermentation process.• Mutant strains produced FOS mixtures with high concentration of 6-kestose.• A new route to produce tailor-made FOS mixtures was presented.

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Microbial production of medium chain fructooligosaccharides by recombinant yeast secreting bacterial inulosucrase

Cited by 15 publications

References 20 publications

Quantification of Fructans in Bioethanol Stillage Based On a Simplified Analytical Method

Quantification of Fructans in Bioethanol Stillage Based On a Simplified Analytical Method

Fructan Biosynthesis by Yeast Cell Factories

Tailoring fructooligosaccharides composition with engineered Zymomonas mobilis ZM4

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